The PR2000-H-S is the trial / sample-size SKU of the Accuris qMAX Green High ROX qPCR Master Mix family, 20-reaction pack for first-use evaluation of the High ROX chemistry on ABI 7000-series Standard-block cyclers before committing to the larger production-scale packs (PR2000-H-100 at $84 / -500 at $366 / -1000 at $672). At $14 list price, the sample SKU enables labs to validate cycler-compatibility and qPCR performance on their specific assay before scaling.
Key Specifications
| SKU | PR2000-H-S |
| Type | Sample / trial pack (qMAX Green qPCR Master Mix, High ROX) |
| Pack quantity | 20 reactions |
| ROX level | High ROX / ABI 7300 / 7500 Standard / 7700 / 7900 cyclers |
| Per-reaction cost (sample) | $0.69 |
| Sister sample SKUs (all $14) | PR2000-N-S (No ROX, sample 20 rxn), PR2000-L-S (Low ROX, sample 20 rxn) |
| Production-scale follow-on | PR2000-H-100 ($84), PR2000-H-500 ($366), PR2000-H-1000 ($672) |
| List price | $14 |
ROX-Variant Sample Triad: Cycler Compatibility Validation
Three matched-price ROX sample SKUs at $14 each enable a complete cycler-compatibility validation:
| Sample SKU | ROX | Compatible cycler family |
| PR2000-N-S | No ROX | Bio-Rad CFX, Roche LightCycler, Eppendorf realplex, Stratagene Mx, Qiagen Rotor-Gene |
| PR2000-L-S | Low ROX | ABI ViiA 7, QuantStudio 6/7/12K, ABI 7500 Fast block |
| PR2000-H-S (this product) | High ROX | ABI 7300 / 7500 Standard / 7700 / 7900 |
Procurement strategy. Labs unsure of cycler ROX requirement can order all three samples ($42 total) for definitive cycler-compatibility confirmation, important because picking the wrong ROX level produces noisy / failed real-time fluorescence traces.
What 20 Reactions Supports: Validation Plan
A typical 20-reaction validation plan for the PR2000-H-S sample:
| Validation step | Reactions used |
| Standard curve (5-point, 3 replicates) | 15 |
| No-template control (NTC) | 2 |
| Positive control (1 replicate × 2 templates) | 2 |
| Reserve for re-test | 1 |
| Total | 20 |
A 20-reaction sample supports a complete first-use validation: confirm cycler-compatibility (passive ROX normalization, qMAX Green fluorescence detection), assay performance (Ct value reproducibility, dynamic range), and primer design (no NTC contamination, expected amplification).
Application Profile
qMAX Green qPCR first-time evaluation on ABI 7000-series Standard-block cyclers: labs evaluating the Accuris qMAX Green chemistry for the first time use the sample to validate cycler-compatibility before bulk procurement.
ROX-variant cycler-compatibility validation. Labs running paired ROX-variant samples confirm correct ROX level for their cycler.
Method-development qPCR. Protocol-development workflows where assay performance needs validation before scaling.
Sales / training samples. Sales reps and training programs use the sample for hands-on customer demonstrations.
Ordering & Configuration
Single-use sample SKU. The 20-reaction pack supports a meaningful validation run; not intended for routine production use.
Cycler-compatibility confirmation. Critical that the sample matches the lab's qPCR cycler's passive-reference-dye requirements.
Pair with template / primers / water. Operator supplies template, primers, and water (qMAX Green is intercalating-dye chemistry; no probe required).
Storage. -20 °C, protect from light.
Confirm at quote: cycler model and ROX requirement, evaluation intent (vs production-pack ordering), workflow application, and lead time.
Warranty: Per Accuris standard reagent terms. Confirm at quote.
Accuris qMAX Green qPCR sample triad (all $14):
- PR2000-N-S, No ROX (Bio-Rad CFX, Roche, Stratagene Mx, etc.)
- PR2000-L-S: Low ROX (ABI ViiA 7, QuantStudio Fast, etc.)
- PR2000-H-S (this product): High ROX (ABI 7300 / 7500 Standard / 7700 / 7900)
Pick the matching ROX-variant sample for cycler-compatibility validation; order all three for definitive compatibility confirmation.
