The PR2000-H-100 is the High ROX formulation of the Accuris qMAX Green qPCR Master Mix — sold as 100-reaction pack, the smallest production-scale pack in the family. Per the Accuris Life Science Reagents Catalog, the qMAX Green family is a single-tube ready-to-use 2X master mix with Hot Start Taq DNA Polymerase included, proprietary intercalating green fluorescent dye for minimal PCR inhibition & optimal fluorescence, and inert blue tracking dye to assist in pipetting visualization. The High ROX (-H) formulation designates compatibility with ABI / Applied Biosystems thermocycler platforms that require High ROX passive reference dye (typically ABI 7300 / 7500 / 7700 / 7900 platforms).
Key Specifications
| SKU | PR2000-H-100 |
| Type | qMAX Green qPCR Master Mix — High ROX formulation |
| Pack quantity | 100 reactions |
| PCR mode | Real-time qPCR with intercalating green fluorescent dye chemistry (SYBR Green / qMAX Green class) |
| ROX level | High ROX — compatibility with ABI 7300 / 7500 / 7700 / 7900 thermocyclers |
| Master mix composition | 2X concentrated mix + Hot Start Taq DNA Polymerase + proprietary intercalating green fluorescent dye + inert blue tracking dye |
| Hot Start chemistry | Included — antibody-mediated activation prevents non-specific amplification at qPCR setup |
| Detection chemistry | Intercalating dye binds dsDNA, fluoresces during amplification — quantitative real-time fluorescence readout |
| Dynamic range | Broad — early Ct values across a wide template-concentration range per brochure |
| Sister SKUs (ROX variants) | PR2000-N-* (No ROX), PR2000-L-* (Low ROX), PR2000-H-* (this product family) |
| Sister SKUs (pack sizes) | PR2000-H-500 (500 rxn), PR2000-H-1000 (1000 rxn, $672), PR2000-H-25BLK / PR2000-H-50BLK (bulk packs) |
| List price | $84 |
ROX Formulation Selection — Pick the Right Variant for the Cycler
The qMAX Green family includes three ROX-level formulations (per brochure: "Available in No ROX, Low ROX, & High ROX formulations") — the operator picks the variant matching the qPCR cycler's passive-reference-dye requirements:
| ROX Formulation | Suffix | Compatible cyclers |
| No ROX | -N | Bio-Rad CFX, Roche LightCycler, Eppendorf realplex, Stratagene Mx, Qiagen Rotor-Gene, and other cyclers that do not use passive reference dye |
| Low ROX | -L | ABI ViiA 7, QuantStudio 6/7/12K, ABI 7500 Fast (Fast block), and other cyclers requiring low ROX |
| High ROX (this product) | -H | ABI 7300, 7500 (Standard block), 7700, 7900 and other cyclers requiring high ROX |
Pick the High ROX (-H) variant when: the qPCR cycler is in the ABI 7000-series Standard-block family (7300 / 7500 Standard / 7700 / 7900) or other cyclers requiring high passive reference dye concentration.
Pick the wrong ROX level → real-time fluorescence normalization fails or produces noisy traces; always confirm cycler-specific ROX requirement at order.
Per Brochure qMAX Green qPCR Mix Features
Per the Accuris Life Science Reagents Catalog, each tube includes Accuris Hot Start Taq DNA Polymerase, qMAX Green proprietary fluorescent dye, dNTPs, and optimized buffer for maximum efficiency. The catalog highlights:
- Single tube formulation: ready-to-use 2X master mix (includes Hot Start Taq DNA Polymerase)
- Proprietary intercalating green fluorescent dye for minimal PCR inhibition and optimal fluorescence
- Inert blue tracking dye to assist visualization during pipetting
- Early Ct values and detection across a broad dynamic range
PR2000-H Pack-Size Family
| SKU | Reactions | List price | Per-reaction cost |
| PR2000-H-100 (this product) | 100 | $84 | $0.84/rxn |
| PR2000-H-500 | 500 | (sister pricing) | (sister economics) |
| PR2000-H-1000 | 1000 | $672 | $0.67/rxn |
| PR2000-H-25BLK | 2500 | (bulk pricing) | (bulk economics) |
| PR2000-H-50BLK | 5000 | (bulk pricing) | (bulk economics) |
Pick the 100-reaction pack when: workflow throughput is moderate (100–500 qPCR reactions per quarter); step to 1000-rxn or BLK bulk for higher throughput.
Why qMAX Green vs Standard SYBR Green Master Mix
The qMAX Green family is engineered as an alternative to standard SYBR Green qPCR master mixes. Its proprietary green-fluorescent intercalating dye is designed for minimal PCR inhibition — an advantage over SYBR Green, which can inhibit at high template concentrations — while still delivering optimal fluorescence with early Ct values and a broad dynamic range. The included Hot Start Taq uses antibody-mediated chemistry to prevent non-specific amplification at qPCR setup, and the inert blue tracking dye lets the operator see the master mix in the well during plate loading without affecting fluorescence detection.
Application Profile
Real-time qPCR for gene expression analysis — the workhorse application. Quantitative mRNA / cDNA expression measurement on ABI 7000-series cyclers requiring High ROX.
Pathogen detection qPCR — research-use viral / bacterial nucleic-acid detection workflows.
Genotyping qPCR (HRM-compatible workflows) — allelic discrimination and high-resolution melt workflows on ABI 7500-series cyclers.
Method-development qPCR — primer design validation, assay development.
Multi-target panel qPCR — multiplex SYBR-class qPCR panels.
Educational laboratory qPCR exercises — molecular-biology coursework introducing real-time quantitative PCR concepts.
Ordering & Configuration
Per-reaction workflow consumable. Each qPCR reaction uses 12.5 µL of the 2X master mix (confirm exact volume in product insert).
Cycler-compatibility confirmation at order. Critical that the High ROX variant matches the lab's qPCR cycler's passive-reference-dye requirements. Confirm cycler model + Standard-vs-Fast-block configuration at order.
Storage. qPCR reagent storage applies — typically -20 °C; protect from light to preserve fluorescent dye chemistry.
Pair with template / primers / probe (if applicable) / water. The master mix includes the polymerase + buffer + dNTPs + intercalating dye + ROX; operator supplies template and primers. (Note: qMAX Green is intercalating-dye chemistry — no probe required for SYBR-class detection.)
Confirm at quote: cycler ROX requirement (High vs Low vs No), workflow throughput (drives pack-size selection), workflow application (gene expression / pathogen detection / genotyping), evaluation pairing (already-authored PR2000-N-25BLK / -50BLK bulk packs available for high-throughput facilities), and lead time.
Warranty: Per Accuris standard reagent terms — confirm at quote.
Accuris PR2000 qMAX Green qPCR Mix family — ROX variant selection:
- PR2000-N-* — No ROX (Bio-Rad CFX, Roche, Stratagene Mx, etc.)
- PR2000-L-* — Low ROX (ABI ViiA 7, QuantStudio Fast block, etc.)
- PR2000-H-* (this product family) — High ROX (ABI 7300 / 7500 Standard / 7700 / 7900)
Pick the High ROX variant for ABI 7000-series Standard-block cyclers; sister ROX variants cover the Low ROX and No ROX cycler families. Step to PR2001 family for qMAX Probe (TaqMan-compatible) qPCR chemistry.
