The PR2110-50 is the entry pack of the Accuris qMAX First Strand cDNA Synthesis Flex Kit family, a 4-tube cDNA synthesis kit with separate random hexamer and oligo dT primer tubes for operator-selectable primer chemistry and high-concentration RTase (200 U/µL) supporting the broadest RNA input range in the Accuris cDNA synthesis family (10 pg – 2.0 µg). Per the Accuris Life Science Reagents Catalog: *"Four-tube formulation for greater flexibility in assay design. Ideal for 10pg – 2.0μg of input RNA."*
Key Specifications
| SKU | PR2110-50 |
| Type | First-strand cDNA synthesis Flex Kit (4-tube format) |
| Pack quantity | 50 reactions |
| Workflow | First-strand cDNA synthesis from RNA template (flexible primer-chemistry selection) |
| Kit contents | 1 tube of RTase (200 U/µL; potent RNase inhibitor included) + 1 tube of 5X buffer (5 mM dNTPs, 15 mM MgCl₂, stabilizers, enhancers) + 1 tube of random hexaprimers + 1 tube of oligo dT primers |
| RTase concentration | 200 U/µL (20× higher than PR2100-C's 10 U/µL) |
| RNA input range | 10 pg – 2.0 µg (broadest in Accuris cDNA synthesis family) |
| Primer chemistry flexibility | Random hexamers AND oligo dT primers in separate tubes, operator selects per workflow |
| Per-reaction cost | $2.26 per reaction |
| Sister SKUs | PR2110-200 (200 reactions, $455), PR2110-S (sample, 5 reactions, $13) |
| Sister kit (2-tube format) | PR2100-C-25 / -100 / -250 (qMAX cDNA Synthesis Kit, mixed primers in buffer) |
| List price | $113 |
Why "Flex": Primer-Chemistry Selectability
The PR2110 Flex Kit's separate-primer-tube format is the key workflow advantage vs the PR2100-C Synthesis Kit's mixed-primer-in-buffer format:
| Workflow | Primer chemistry | Why |
| Polyadenylated mRNA → cDNA | Oligo dT only | Oligo dT primer binds polyA tail; produces full-length mRNA cDNA preferentially |
| Total RNA → cDNA (including non-polyadenylated species) | Random hexamer only | Random hexamers bind throughout RNA (captures rRNA, tRNA, miRNA precursors, non-polyA mRNA) |
| Mixed RNA → cDNA (default workflow) | Both primers | Comprehensive cDNA coverage |
| Specific-region cDNA | Gene-specific primer (operator-supplied) | Operator-supplied gene-specific primer for targeted cDNA |
The Flex Kit lets the operator pick the primer chemistry per workflow. The PR2100-C Synthesis Kit's mixed-primer buffer doesn't support primer-only workflows. Both primers are always present.
Why 200 U/µL RTase: Low-Input RNA Support
The PR2110 Flex Kit's 200 U/µL RTase concentration (vs PR2100-C's 10 U/µL) supports the broadest RNA input range in the Accuris cDNA synthesis family: 10 pg – 2.0 µg.
Workflow context:
- 10 pg lower bound: supports single-cell RNA-Seq, FFPE-extracted RNA, exosomal RNA, ancient / forensic RNA
- 2.0 µg upper bound: supports bulk RNA-Seq library construction, microarray prep, gene expression panel multi-target downstream qPCR
The PR2100-C Synthesis Kit's 10 U/µL RTase concentration is sufficient for standard ng-range RNA inputs, typical bulk-tissue mRNA prep, but does not support the low-input workflows where the PR2110 Flex Kit is the right pick.
PR2100-C vs PR2110: Pick the Right Kit
| Feature | PR2100-C qMAX cDNA Synthesis Kit | PR2110 qMAX First Strand cDNA Flex Kit (this product family) |
| Tube count | 2 tubes (RTase + buffer-with-primers) | 4 tubes (RTase + buffer + random hexamers + oligo dT) |
| RTase concentration | 10 U/µL | 200 U/µL |
| RNA input range | Standard ng-range | 10 pg – 2.0 µg (low-input to bulk) |
| Primer chemistry | Mixed in buffer | Operator-selectable |
| Workflow flexibility | Routine cDNA synthesis | Method-development + flexibility |
| Per-rxn cost (entry pack) | $5.27 (PR2100-C-25) | $2.26 (PR2110-50) |
| Best fit | Routine bulk-tissue mRNA → cDNA | Low-input RNA, primer-chemistry-flexible, method-development |
Per-reaction cost note: The PR2110 Flex Kit is significantly cheaper per reaction ($2.26 vs $5.27), likely reflecting that the Flex Kit's 4-tube format spreads cost across the larger pack quantity (50 vs 25) and the operator supplies the primer-chemistry selection (vs Accuris pre-mixing both primers in the PR2100-C buffer).
PR2110 Pack-Size Family
| SKU | Reactions | List price | Per-reaction cost |
| PR2110-S | 5 | $13 | $2.54 |
| PR2110-50 (this product) | 50 | $113 | $2.26 |
| PR2110-200 | 200 | $455 | $2.27 |
Per Brochure Applications
Per Accuris Life Science Reagents Catalog, PR2110 First Strand cDNA Flex Kit applications include:
- cDNA Library Generation. NGS library construction starting material (especially low-input)
- Microarrays. CDNA template for microarray hybridization
- Gene Expression Analysis. CDNA for downstream RT-PCR or RT-qPCR quantification
- 2-Step RT-PCR / RT-qPCR. Separate cDNA synthesis + PCR / qPCR workflow
Application Profile
Low-input RNA workflows. Single-cell RNA-Seq, FFPE-extracted RNA, exosomal RNA prep where the 10 pg lower input bound is the key workflow enabler.
Method-development workflows. Protocol-development workflows where primer-chemistry selection (oligo dT only vs random hexamer only vs both) supports protocol optimization.
NGS library construction with primer-chemistry control. RNA-Seq workflows where the operator wants to pick the primer chemistry per library design.
Clinical-research diagnostic workflows. Clinical-research workflows where FFPE-extracted RNA inputs benefit from the high-concentration RTase + low-input range.
Multi-investigator shared facilities. Shared facilities where different investigators need different primer chemistries benefit from the Flex Kit's selectability.
Educational laboratory cDNA exercises. Molecular-biology coursework demonstrating primer-chemistry selection concepts.
Ordering & Configuration
Per-reaction workflow consumable. Each cDNA synthesis reaction consumes proportional reagent.
Storage. -20 °C, RNase-free handling required.
Pair with RNA template + downstream PCR / qPCR reagents. The kit produces cDNA; operator supplies RNA template, primer chemistry selection (which primers to use), and downstream PCR / qPCR master mix.
Confirm at quote: workflow throughput (50-rxn vs 200-rxn pack), Flex Kit vs Synthesis Kit decision (vs PR2100-C family), RNA input range (low-input vs standard), evaluation interest (PR2110-S sample at $13), and lead time.
Warranty: Per Accuris standard reagent terms. Confirm at quote.
Accuris PR2110 qMAX First Strand cDNA Flex Kit family:
- PR2110-S: Sample, 5 reactions ($13, $2.54/rxn)
- PR2110-50 (this product): 50 reactions ($113, $2.26/rxn)
- PR2110-200: 200 reactions ($455, $2.27/rxn)
Pick the Flex Kit for low-input RNA workflows, primer-chemistry flexibility, or method-development; step to PR2100-C qMAX cDNA Synthesis Kit for routine bulk-tissue cDNA synthesis with mixed primers.
