The PR1400-FLS-100 is the standalone Fast Extraction Lysis Solution — sold as 100-reaction pack for component-procurement workflows where the lab pairs the lysis solution with their preferred master mix (vs ordering the complete PR1400-FE Fast Extraction PCR Kit which includes both lysis solution AND master mix). The PR1400-FLS family supports labs that already have Accuris (or compatible) master mix in stock and only need the lysis chemistry component.
Author confidence note (MEDIUM, FQ-017 pattern): The PR1400 family is not in the available brochure corpus. Page authored from universe label semantics. Confirm exact lysis solution chemistry, sample-type compatibility, master-mix pairing recommendations, and workflow protocol with Accuris technical support at first order.
Key Specifications
| SKU | PR1400-FLS-100 |
| Type | Standalone Fast Extraction Lysis Solution |
| Pack quantity | 100 reactions |
| Workflow position | Lysis component — pair with operator-supplied master mix for complete extraction + PCR workflow |
| Per-reaction cost | $0.69 per reaction |
| Sister SKUs | PR1400-FLS-400 (400 reactions, $235), PR1400-FLS-S (sample 16 reactions, $13), PR1400-FE-100 (complete kit with master mix) |
| List price | $69 |
When to Pick FLS Standalone vs FE Complete Kit
The PR1400 family's two procurement paths:
| Path | SKUs | Cost per 100 reactions | Best fit |
| Complete kit | PR1400-FE-100 ($128) | $128 per 100 rxn (kit) | Convenience-prioritized; lab does not have master mix in stock; new-to-Fast-Extraction workflow |
| Standalone Lysis Solution (this product family) | PR1400-FLS-100 ($69) + operator master mix | $69 + master mix cost per 100 rxn | Lab has Accuris Hot Start Master Mix or HF Master Mix in stock; cost-optimized procurement; workflow-flexibility |
Component-procurement economics example:
- PR1400-FE-100 complete kit: $128 per 100 rxn
- PR1400-FLS-100 + PR1001-HS-200 (provides 200 rxn of master mix): $69 + $174 = $243 for 100 lysis × 200 master mix = effectively $0.69 lysis + $0.87 master mix = $1.56 per Fast Extraction reaction (compared to $1.28 for complete kit)
- Net: complete kit is cheaper per Fast Extraction reaction — but the standalone lysis solution lets the lab use already-stocked master mix without dual-procurement overhead
Pick FLS standalone when: the lab has master mix already in stock (no need for fresh procurement) OR wants to pair the lysis solution with HF or HFHS master mix for HF-grade Fast Extraction workflow.
PR1400-FLS Pack-Size Family
| SKU | Reactions | List price | Per-reaction cost |
| PR1400-FLS-S | 16 | $13 | $0.81 |
| PR1400-FLS-100 (this product) | 100 | $69 | $0.69 |
| PR1400-FLS-400 | 400 | $235 | $0.59 |
Pick the 100-rxn pack when: workflow throughput is moderate (100 lysis steps per quarter); step to 400-rxn for production-scale; step to sample for first-use evaluation.
Design Architecture — Lysis + Master Mix Pairing
Standard workflow with PR1400-FLS standalone:
- Sample lysis — sample + PR1400-FLS lysis solution (operator-supplied lysis protocol, brochure pending)
- Direct PCR setup — lysate + operator-supplied master mix (typically Hot Start Taq Master Mix per Accuris recommendation)
- Thermal cycling — standard PCR cycling
- Gel readout (with Red Dye Master Mix for direct gel load, or with separate loading dye)
Master mix pairing recommendations (likely):
- PR1001-HS-200 / -1000 (Hot Start Taq Master Mix) — recommended pairing for non-specific suppression in extracted lysate workflows
- PR1001-HSR-200 / -1000 (Hot Start Red Dye) — direct-gel-load workflow option
- PR1001-HF-200 / -500 (HF Master Mix) — HF-grade Fast Extraction for cloning / sequencing-prep workflows
- PR1001-HFHS-200 / -500 (HF + Hot Start) — combined-chemistry HF Fast Extraction
Application Profile
Streamlined DNA extraction + PCR workflows — same workflow applications as the complete PR1400-FE kit (general-purpose template-prep PCR).
Lab-flexible master mix pairing — labs that want to pair the lysis chemistry with non-default master mix (e.g., HF for cloning workflows, HFHS for combined-chemistry workflows).
Cost-optimized procurement — labs already stocking Accuris master mix in bulk benefit from the standalone lysis solution at lower per-reaction lysis cost.
Method-development workflows — protocol-development workflows where the lysis-vs-master-mix variables can be optimized independently.
Educational laboratory teaching — molecular-biology coursework demonstrating the modular extraction + PCR workflow design.
Ordering & Configuration
Per-reaction workflow consumable. Each Fast Extraction lysis step uses proportional reagent. The 100-rxn pack supports 100 lysis steps before reorder.
Pair with master mix. Confirm master mix inventory at order — the PR1400-FLS standalone is workflow-incomplete without master mix. Recommended pairings: PR1001-HS family (Hot Start), PR1001-HF family (HF for cloning workflows).
Confirm at quote: workflow throughput sizing, master mix inventory or pairing-procurement plan, sample-type compatibility (FQ-017 pending brochure pull), and lead time.
Warranty: Per Accuris standard reagent terms — confirm at quote.
Accuris PR1400 Fast Extraction Lysis Solution standalone family:
- PR1400-FLS-S — Sample, 16 reactions ($13, $0.81/rxn)
- PR1400-FLS-100 (this product) — 100 reactions ($69, $0.69/rxn)
- PR1400-FLS-400 — 400 reactions ($235, $0.59/rxn)
- PR1400-FE-* — Complete kit (lysis + master mix included)
Pick the standalone Lysis Solution for component-procurement workflows; step to the complete PR1400-FE kit for single-procurement convenience.
