The EB1100 is the Tris-Glycine-SDS (TGS) buffer powder in the Accuris QuickSilver Powdered Buffer Pack family, sold as 50 pre-measured pouches, each making 1 L of 1× working buffer when dissolved in water. Tris-Glycine-SDS is the most common running buffer for SDS-PAGE protein electrophoresis, the workhorse buffer chemistry for routine protein-electrophoresis workflows across academic research, biotech R&D, clinical-research, and teaching-lab applications.
Per brochure: *"Tris-Glycine-SDS is one of the most common running buffers used for SDS-PAGE. A 1X solution contains 25 mM Tris, 192 mM Glycine and 0.1% SDS."*
Key Specifications
| SKU | EB1100 |
| Type | Tris-Glycine-SDS (TGS) buffer powder, pre-measured pouches |
| Pack quantity | 50 pouches per box |
| Per-pouch yield | 1 L of 1× working buffer (each pouch = 1 L) |
| 1× formulation | 25 mM Tris, 192 mM Glycine, 0.1 % SDS |
| Workflow application | SDS-PAGE protein electrophoresis (running buffer) |
| Best fit | Routine SDS-PAGE workflows across the full protein-MW range |
| Quality / handling | Nuclease-free, protease-free, shelf-stable; preprinted ID labels included |
| List price | $202 (50 pouches) |
SDS-PAGE Buffer Selection: TGS vs MES vs MOPS
The QuickSilver protein-electrophoresis buffer family includes three SDS-PAGE running buffer chemistries optimized for different protein-MW ranges:
| Buffer | 1× formulation | Run speed | Best protein-MW range |
| Tris-Glycine-SDS (TGS) (this product) | 25 mM Tris, 192 mM Glycine, 0.1 % SDS | Standard | Workhorse buffer (full MW range) |
| Tris-MES-SDS | 50 mM Tris, 50 mM MES, 0.1 % SDS | Faster runs | Small-to-medium proteins |
| Tris-MOPS-SDS | 50 mM Tris, 50 mM MOPS, 0.1 % SDS, 1 mM EDTA | Slower runs | Medium-to-large proteins |
Pick Tris-Glycine-SDS (EB1100) when:
- Routine SDS-PAGE workflows where standard run speed and MW range coverage are sufficient
- Teaching laboratory exercises (the most-recognized SDS-PAGE buffer chemistry across textbooks and protocols)
- Workflows where the lab maintains compatibility with published protocols using TGS
- Mixed-MW-range workflows where neither MES nor MOPS specifically benefits the application
Step to Tris-MES-SDS when small-medium proteins benefit from faster runs (typical: 10–70 kDa target proteins).
Step to Tris-MOPS-SDS (EB1102) when medium-large proteins benefit from slower, higher-resolution runs (typical: 50–200 kDa target proteins).
QuickSilver Workflow Advantage
Per the QuickSilver Powdered Buffer Pack brochure: each pre-measured pouch eliminates the weighing → dissolving → pH-adjustment → verification sequence of traditional buffer prep, replacing it with a 2-minute open-empty-stir workflow. For Tris-Glycine-SDS specifically:
- No SDS-handling complexity. Pre-measured powder eliminates SDS dust-handling concerns common with bulk-SDS weighing
- No pH adjustment. Formulation delivers the correct pH on dissolution (typically pH ~8.3 for 1× TGS)
- Consistent molarity. Pre-measured powder eliminates per-component weighing-error variance that affects SDS-PAGE band-mobility reproducibility
Application Profile
Routine SDS-PAGE protein analysis. The workhorse application across academic research, biotech R&D, clinical-research, and pharmaceutical-quality workflows.
Western blot transfer-buffer base. Tris-Glycine is also the base chemistry for many Western-blot transfer buffers (with methanol added per protocol); the EB1100 powder can serve dual SDS-PAGE-running and Western-transfer roles.
Protein-purification fraction QC. Protein-purification workflows running SDS-PAGE QC on column fractions benefit from the rapid QuickSilver buffer-prep workflow.
Teaching laboratory protein-electrophoresis. Undergraduate and graduate biochemistry / molecular-biology programs running SDS-PAGE coursework benefit from the simplified buffer-prep workflow.
Multi-investigator shared-equipment facilities. Shared SDS-PAGE equipment in core facilities benefits from QuickSilver pouches (each investigator preps fresh buffer per session, no shared-stock-solution drift between investigators).
Ordering & Configuration
Per-pouch workflow consumable. Each pouch makes 1 L of 1× buffer; pack of 50 pouches supports 50 L of running-buffer prep, substantial throughput for most academic / clinical-research labs but plan procurement to match production-throughput labs' faster consumption.
Pre-printed ID labels included. Each pack ships with preprinted bottle labels for buffer bottles.
Confirm at quote: workflow buffer-volume consumption, SDS-PAGE protein-MW range (sister buffer EB1102 may be a better fit for large-protein workflows), shelf-storage capacity, and lead time.
Warranty: Per Accuris standard reagent terms. Confirm at quote.
Accuris QuickSilver SDS-PAGE buffer family:
- EB1100 (this product): Tris-Glycine-SDS (workhorse, 50 pouches)
- EB1102: Tris-MOPS-SDS (medium-large proteins, slower runs, 50 pouches)
- (EB1101 Tris-MES-SDS for small-medium proteins, faster runs; see Accuris reagents catalog if available)
Pick TGS for routine SDS-PAGE; step to MOPS for medium-large proteins or MES for small-medium proteins.
