The EB1203 is the TBS-T (Tris-buffered saline with Tween-20) buffer powder in the Accuris QuickSilver Powdered Buffer Pack family — sold as 100 pre-measured pouches, each making 1 L of 1× working buffer when dissolved in water. TBS-T is the standard Western-blot membrane wash buffer — the Tween-20 detergent supplements the base TBS chemistry to reduce non-specific antibody binding to nitrocellulose / PVDF membrane surfaces during the multi-wash steps of Western-blot workflows.
Per brochure: *"1X TBST contains 0.1% Tween-20."* The base TBS formulation is identical to EB1202 (137 mM NaCl, 2.7 mM KCl, 50 mM Tris) — the difference is the addition of 0.1 % Tween-20.
Key Specifications
| SKU | EB1203 |
| Type | TBS-T (TBS + 0.1 % Tween-20) buffer powder, pre-measured pouches |
| Pack quantity | 100 pouches per box |
| Per-pouch yield | 1 L of 1× working buffer (each pouch = 1 L) |
| 1× formulation | 137 mM NaCl, 2.7 mM KCl, 50 mM Tris, 0.1 % Tween-20 |
| Workflow application | Western-blot membrane wash, antibody dilution for Western, immunoassay wash, phosphate-free wash workflows |
| Sister buffer | EB1202 (TBS without Tween-20 — same $254 price) |
| Quality / handling | Nuclease-free, protease-free, shelf-stable; preprinted ID labels included |
| List price | $254 (100 pouches) |
Why TBS-T for Western-Blot Membrane Wash
Western-blot workflows transfer SDS-PAGE-separated proteins onto nitrocellulose or PVDF membranes, then probe with primary and secondary antibodies to detect target proteins. Between each protocol stage (after blocking, after primary antibody, after secondary antibody), multiple wash steps remove unbound antibody from the membrane.
Tween-20 at 0.1 % in the wash buffer:
- Disrupts hydrophobic interactions between antibodies and the membrane surface
- Reduces non-specific antibody binding to membrane background (lower background signal)
- Maintains specific antibody-antigen binding at the target band
- Improves signal-to-noise ratio in the final detection (chemiluminescence, fluorescence, or chromogenic)
The 0.1 % concentration in TBS-T is 2× the 0.05 % concentration in PBS-T (EB1201) — Western-blot membrane wash typically requires more aggressive detergent action than ELISA plate wash because membranes have more hydrophobic surface area than polystyrene plates and benefit from stronger detergent disruption.
Western-Blot Workflow — Per-Step Buffer Volumes
Typical Western-blot workflow buffer consumption (per blot, mini-format gel ~7 × 9 cm membrane):
| Protocol step | TBS-T volume per blot |
| Wash after blocking | 3 × 50 mL = 150 mL |
| Primary antibody incubation (in TBS-T diluent) | 5–20 mL (overnight) |
| Wash after primary antibody | 3–5 × 50 mL = 150–250 mL |
| Secondary antibody incubation (in TBS-T diluent) | 5–20 mL (1 hour) |
| Wash after secondary antibody | 3–5 × 50 mL = 150–250 mL |
| Total per blot | ~460–690 mL TBS-T |
A pack of 100 pouches (100 L) supports ~145–217 Western blots before reorder.
EB1203 vs EB1202 — Pick the Right Buffer
| Buffer | Workflow |
| EB1203 TBS-T (this product) | Western-blot membrane wash, antibody incubation buffers, immunoassay wash (phosphate-free workflows) |
| EB1202 TBS | Antibody dilution for IF / IHC (no detergent needed), IP wash buffer, phosphate-free workflows without wash detergent |
Pick TBS-T (EB1203) when: the workflow includes Western-blot membrane wash steps or other immunoassay wash steps where Tween-20 detergent improves signal-to-noise.
Pick TBS (EB1202) when: the workflow does not include detergent-mediated wash steps.
TBS-T vs PBS-T — Detergent Concentration Differential
| Buffer | Tween-20 concentration | Best fit |
| EB1203 TBS-T (this product) | 0.1 % | Western-blot membrane wash — more aggressive detergent action |
| EB1201 PBS-T | 0.05 % | ELISA plate wash — gentler detergent action |
The 2× detergent differential between TBS-T and PBS-T reflects the different surface chemistries: membranes (more hydrophobic) benefit from higher Tween-20; polystyrene plates (less hydrophobic) work well at the lower concentration.
Application Profile
Western-blot membrane wash workflows — the workhorse application; TBS-T is the standard wash buffer for nitrocellulose and PVDF membrane workflows across academic, biotech, and clinical-research labs.
Phosphoprotein Western blot workflows — anti-phospho antibody Western blots require phosphate-free buffers; TBS-T is the standard choice for these workflows.
Alkaline-phosphatase (AP) detection systems — AP-conjugated secondary antibody Western blots use TBS-T to avoid phosphate competition with the AP substrate.
Antibody dilution buffer for Western — primary and secondary antibody dilutions in Western-blot workflows often use TBS-T as the diluent.
Multi-investigator Western-blot facilities — shared Western-blot equipment in core facilities benefits from per-pouch fresh TBS-T prep.
Educational laboratory Western-blot exercises — undergraduate / graduate biochemistry / molecular-biology programs running Western-blot coursework benefit from the simplified buffer-prep workflow.
Ordering & Configuration
Per-pouch workflow consumable. Each pouch makes 1 L of 1× buffer; pack of 100 pouches supports ~145–217 Western blots.
Pre-printed ID labels included. Each pack ships with preprinted bottle labels for buffer bottles.
Confirm at quote: workflow buffer-volume consumption (Western-blot throughput drives pack-quantity needs), TBS-vs-TBS-T selection, phosphate-sensitivity of detection chemistry, shelf-storage capacity, and lead time.
Warranty: Per Accuris standard reagent terms — confirm at quote.
Accuris QuickSilver Western-blot / immunoassay buffer family:
- EB1200 — PBS (general physiological, 100 pouches, $254.00)
- EB1201 — PBS-T (ELISA wash, 0.05 % Tween-20, 100 pouches, $254.00)
- EB1202 — TBS (Tris-buffered saline, 100 pouches, $254)
- EB1203 (this product) — TBS-T (Western-blot wash, 0.1 % Tween-20, 100 pouches, $254)
Pick TBS-T for Western-blot membrane wash workflows; pair with EB1202 TBS for non-detergent steps in the same workflow.
