The EB1202 is the TBS (Tris-buffered saline) buffer powder in the Accuris QuickSilver Powdered Buffer Pack family — sold as 100 pre-measured pouches, each making 1 L of 1× working buffer when dissolved in water. TBS is the Tris-based alternative to PBS for Western-blot, immunoassay, and antibody-based workflows where the narrow-pH-range Tris buffering provides better assay performance than the broader-range phosphate buffering of PBS. TBS is particularly preferred for phosphoprotein detection workflows where phosphate-containing PBS could interfere with anti-phospho antibody binding or alkaline-phosphatase substrate detection.
Per brochure: *"TBS and TBST are used to maintain pH within a very narrow range for a variety of applications including western blotting and immunoassays. The final concentration of a 1X working solution is 137 mM NaCl, 2.7 mM KCl, 50 mM Tris."*
Key Specifications
| SKU | EB1202 |
| Type | TBS (Tris-buffered saline) buffer powder, pre-measured pouches |
| Pack quantity | 100 pouches per box |
| Per-pouch yield | 1 L of 1× working buffer (each pouch = 1 L) |
| 1× formulation | 137 mM NaCl, 2.7 mM KCl, 50 mM Tris |
| Workflow application | Western-blot wash buffer, immunoassay buffer, antibody dilution buffer, phosphate-free workflows |
| Sister buffer | EB1203 (TBS-T — TBS supplemented with 0.1 % Tween-20) |
| Quality / handling | Nuclease-free, protease-free, shelf-stable; preprinted ID labels included |
| List price | $254 (100 pouches) |
TBS vs PBS — Pick the Right Buffer Chemistry
| Feature | TBS (this product) | PBS (EB1200) |
| Buffering agent | Tris (50 mM) | Phosphate (10 mM) |
| pH stability range | Narrow, well-controlled around pH 7.4–7.6 | Broader; pH may drift with temperature |
| Phosphate content | None | 10 mM phosphate |
| Phosphoprotein workflows | ✓ Preferred (no phosphate interference) | ✗ Phosphate may interfere |
| Alkaline-phosphatase substrate workflows | ✓ Preferred (no phosphate competition) | ✗ Phosphate competes with substrate |
| Cell-culture / physiological workflows | (Tris can be cytotoxic at extended exposure) | ✓ Preferred (mimics physiological conditions) |
| HPLC / MS workflows | (Tris is volatile, less common in HPLC) | ✓ Preferred (PBS is standard HPLC buffer) |
| Western-blot wash workflows | ✓ Preferred (better pH stability across long-incubation steps) | (Acceptable but TBS often performs better) |
| List price | $254 | $254.00 |
Pick TBS (EB1202) when:
- The workflow detects phosphoproteins (anti-phospho-Ser / anti-phospho-Tyr / anti-phospho-Thr antibodies)
- Alkaline-phosphatase-conjugated detection systems are used (phosphate would compete with the AP substrate)
- Western-blot workflows requiring tight pH control across long incubation steps
- Workflows where phosphate may precipitate with calcium or other divalent cations in samples
Pick PBS (EB1200) when: cell-culture, HPLC, MS, or general physiological workflows are the priority and the workflow does not include phosphoprotein or AP-detection steps.
Application Profile
Western-blot wash and incubation workflows — TBS (or TBS-T with detergent) is the standard Western-blot buffer for membrane washing, antibody incubation, and substrate-development steps.
Phosphoprotein detection workflows — anti-phospho antibody Western blots, IF, and IHC workflows require phosphate-free buffers; TBS is the standard alternative to PBS for these workflows.
Alkaline-phosphatase (AP) detection systems — AP-conjugated secondary antibody Western blots and ELISA workflows use TBS to avoid phosphate competition with the AP substrate (BCIP / NBT or compatible).
ELISA workflows with phosphate-sensitive detection — some ELISA assays use TBS-based wash and dilution buffers when the detection chemistry is phosphate-sensitive.
Immunoprecipitation (IP) wash buffers — many IP protocols use TBS-based wash buffers to maintain consistent pH across the multi-wash steps of the IP workflow.
Educational laboratory Western-blot exercises — undergraduate / graduate biochemistry / molecular-biology programs running Western-blot coursework benefit from the simplified buffer-prep workflow.
EB1202 vs EB1203 — TBS vs TBS-T
| Buffer | Formulation | Workflow |
| EB1202 TBS (this product) | 137 mM NaCl, 2.7 mM KCl, 50 mM Tris | Antibody dilution, IP wash, IHC dilution buffer (no detergent needed) |
| EB1203 TBS-T | TBS + 0.1 % Tween-20 | Western-blot membrane wash, immunoassay wash (detergent reduces non-specific binding) |
Pick TBS (EB1202) when: workflows do not include detergent-mediated wash steps. Antibody dilutions for IF / IHC, IP wash steps, ELISA workflows where the lab's protocol does not call for detergent.
Pick TBS-T (EB1203) when: workflows include Western-blot membrane wash steps where the Tween-20 detergent reduces non-specific antibody binding.
Ordering & Configuration
Per-pouch workflow consumable. Each pouch makes 1 L of 1× buffer; pack of 100 pouches supports 100 L of buffer prep.
Pre-printed ID labels included. Each pack ships with preprinted bottle labels for buffer bottles.
Confirm at quote: workflow buffer-volume consumption, TBS-vs-TBS-T selection (detergent vs no-detergent), phosphate-sensitivity of detection chemistry (drives TBS-vs-PBS decision), shelf-storage capacity, and lead time.
Warranty: Per Accuris standard reagent terms — confirm at quote.
Accuris QuickSilver Western-blot / immunoassay buffer family:
- EB1202 (this product) — TBS (Tris-buffered saline, 100 pouches, $254)
- EB1203 — TBS-T (TBS + Tween-20 for Western wash, 100 pouches, $254)
- EB1200 — PBS (general physiological, 100 pouches, $254.00)
- EB1201 — PBS-T (ELISA wash, 100 pouches, $254.00)
Pick TBS for phosphate-free / phosphoprotein workflows; pair with EB1203 TBS-T for Western-blot wash steps.
