The PR4100-100 is the entry pack of the Accuris SmartCheck 1 kb DNA Ladder — the large-fragment range for plasmid analysis, large-amplicon PCR (500 bp – 10 kb+), and other workflows where the smaller-fragment 50 bp / 100 bp ladders' upper range is insufficient. Sold as 500 µL / 100 lanes (5 µL per lane standard loading volume).
For complete chemistry rationale, brochure specifications, and sister-range selection, see the PR4005-100 parent page.
Key Specifications
| SKU | PR4100-100 |
| Type | DNA Ladder — 1 kb resolution (large-fragment range) |
| Pack quantity | 500 µL / 100 lanes |
| Concentration | 0.1 mg/mL |
| Format | Ready-to-use — includes loading buffer & tracking dye |
| Range | 500 bp – 10 kb+ large-fragment range |
| Sister SKUs (same range) | PR4100-500 |
| Sister SKUs (different ranges) | PR4005-100 / -500 (50 bp), PR4010-100 / -500 (100 bp workhorse) |
When to Pick the 1 kb Ladder
| Workflow | Recommended ladder |
| Plasmid analysis (typical 2–10 kb plasmids) | PR4100 (1 kb) — this product family |
| Long-amplicon PCR (500 bp – 5 kb amplicons) | PR4100 (1 kb) |
| Genomic DNA fragment analysis (after partial digestion) | PR4100 (1 kb) |
| Cloning workflow large-insert verification | PR4100 (1 kb) |
| Standard 100 bp – 1 kb PCR products | PR4010 (100 bp workhorse) |
| Short-amplicon (50–500 bp) fine resolution | PR4005 (50 bp) |
Application Profile
Plasmid analysis — the workhorse 1 kb application. Mini-prep, midi-prep, and maxi-prep plasmid sizing on 0.7–1 % agarose gels.
Large-amplicon PCR-product verification — long-range PCR amplicons (1–5 kb) where the 100 bp ladder's 1 kb upper range is insufficient.
Genomic DNA fragmentation analysis — partial restriction-digest fragment patterns from genomic DNA.
Cloning verification with large inserts — confirming insert size after vector + insert ligation.
Library construction QC — fragment-size distribution analysis for NGS library construction.
Educational laboratory plasmid biology demonstrations — molecular-biology coursework introducing plasmid mapping concepts.
Ordering & Configuration
Per-lane workflow consumable. Each gel lane uses 5 µL.
Storage. -20 °C, protect from light.
Confirm at quote: workflow throughput (100-lane vs 500-lane pack), workflow application (plasmid vs PCR vs cloning), gel-stain chemistry compatibility, and lead time.
Warranty: Per Accuris standard reagent terms — confirm at quote.
Accuris SmartCheck DNA Ladder family — 1 kb resolution (large-fragment range):
- PR4100-100 (this product) — 1 kb ladder, 100 lanes
- PR4100-500 — 1 kb ladder, 500 lanes
Pick the 1 kb ladder for plasmid analysis and large-fragment workflows; sister ranges PR4005 (50 bp) and PR4010 (100 bp) cover smaller-fragment workflows.
