The E5001-535 is the 535 nm band-pass photo filter for the Accuris SmartDoc 2.0 Imaging Enclosure (E5001-SD) — used when imaging green fluorescent DNA stains on a UV transilluminator (vs the SmartDoc's standard configuration with blue-light transilluminator + orange filter). The filter is glass-construction with a band-pass spectral profile centered at 535 nm that:
- Blocks UV excitation light — so UV bulbs do not show in the captured image (and so UV does not reach the smartphone camera)
- Eliminates background wavelengths from the excitation source — improving the signal-to-background ratio in the captured gel image
- Transmits green fluorescence emission — the 535 nm pass-band matches the emission peak of green fluorescent DNA stains (SmartGlow, SYBR Green, Gel Green, GelStar, GelGreen, GreenGlo)
Key Specifications
| SKU | E5001-535 |
| Type | Glass band-pass photo filter, 535 nm centered |
| Pass band | 535 nm (green fluorescence emission) |
| Block band | UV (302 nm midrange) and excitation-source background wavelengths |
| Compatible imaging enclosure | E5001-SD SmartDoc 2.0 (and E5001-SDB bundled package) |
| Workflow context | SmartDoc + UV transilluminator + green fluorescent stain |
| Sister filter | E5001-590 (590 nm for EtBr on UV transilluminator) |
| Standard filter (included with SmartDoc) | E5001-ORANGE (for blue-light transilluminator workflow) |
SmartDoc Filter Selection Decision Matrix
The SmartDoc 2.0 enclosure ships with the E5001-ORANGE filter included for the standard blue-light transilluminator + green stain workflow. Two separately-ordered band-pass filters support UV-transilluminator workflows depending on stain chemistry:
| Workflow | Transilluminator | Stain | SmartDoc filter |
| Blue-light + green stain (default) | E4000 SmartBlue | SmartGlow / SYBR Green / Gel Green | E5001-ORANGE (included with SmartDoc) |
| UV + green stain (this filter applies) | E3000 / E3100 / E3200 UV transilluminator | SmartGlow / SYBR Green / Gel Green | E5001-535 (this product) |
| UV + EtBr | E3000 / E3100 / E3200 UV transilluminator | Ethidium bromide | E5001-590 |
| UV + other stains | UV transilluminator | Stain-dependent | Confirm filter compatibility per stain emission spectrum |
Why a Band-Pass Filter for UV + Green Stain Imaging
When the SmartDoc 2.0 is paired with a UV transilluminator instead of the SmartBlue, the standard orange filter (E5001-ORANGE, designed for blue excitation) is not optimal for two reasons:
- UV excitation passes through orange-tint filters — the 302 nm UV light reaches the smartphone camera, potentially damaging the camera sensor over extended exposure and contaminating the captured image with UV-bulb background
- Green-stain emission peaks at 535 nm — a narrow-band filter centered at 535 nm captures the emission with optimal signal-to-background ratio, while the broader orange filter passes more background wavelengths
The E5001-535 band-pass filter addresses both:
- Blocks UV so the smartphone camera is protected and the captured image is UV-bulb-free
- Pass band centered at green emission peak for optimal stain-signal capture
Compatible Stains
Per the SmartDoc 2.0 manual, the E5001-535 is appropriate for green fluorescent stains on UV transilluminator workflows including SmartGlow PS (Pre-Stain), SmartGlow LD (Loading Dye), SYBR Green I and II, SYBR Safe, SYBR Gold, Gel Star, Gel Green, and GreenGlo.
Application Profile
Labs transitioning from EtBr to green stains while keeping UV transilluminator inventory — labs that have moved their stain chemistry from EtBr to SmartGlow / SYBR Green but still use their existing UV transilluminator (vs investing in a SmartBlue) need the E5001-535 to image the new stains correctly.
Multi-stain imaging facilities — facilities running mixed EtBr + green-stain workflows on the same UV transilluminator alternate between E5001-590 (EtBr) and E5001-535 (green-stain) per workflow run.
Sequencing core facilities — facilities running both stain chemistries depending on sample-preparation needs benefit from both band-pass filters in the SmartDoc accessory inventory.
Educational laboratory imaging — teaching labs running mixed stain chemistries on shared UV transilluminator equipment benefit from the green-stain filter for SmartGlow / SYBR Green class exercises.
Ordering & Configuration
Pair with SmartDoc 2.0 + UV transilluminator inventory. The filter is workflow-required for the specific SmartDoc + UV + green-stain combination, not workflow-mandatory for all SmartDoc workflows. Confirm stain chemistry and transilluminator type at order.
Confirm at quote: SmartDoc 2.0 chassis (E5001-SD / E5001-SDB) in inventory, UV transilluminator inventory, gel-stain chemistry, sister-filter inventory (E5001-590 for EtBr workflows), and lead time.
Warranty: Per Accuris standard accessory terms — confirm at quote.
SmartDoc 2.0 photo-filter family:
- E5001-ORANGE — Orange filter for blue-light + green-stain workflow (included with SmartDoc 2.0; replacement available)
- E5001-535 (this product) — 535 nm band-pass for UV + green-stain workflow
- E5001-590 — 590 nm band-pass for UV + EtBr workflow
- E5001-UVBLOCK — UV blocking filter optimized for E3100 myView Compact UV transilluminator
Pick the filter matching the transilluminator + stain combination in use.
