The E5001-590 is the 590 nm band-pass photo filter for the Accuris SmartDoc 2.0 Imaging Enclosure (E5001-SD) — used when imaging ethidium bromide (EtBr) stained DNA gels on a UV transilluminator (the classic UV + EtBr workflow that remains standard in many labs despite the broader industry shift to safer green-fluorescent alternatives). The filter is glass-construction with a band-pass spectral profile centered at 590 nm that matches EtBr's red-orange emission peak — capturing high-resolution EtBr-band images while blocking UV excitation light from reaching the smartphone camera.
Key Specifications
| SKU | E5001-590 |
| Type | Glass band-pass photo filter, 590 nm centered |
| Pass band | 590 nm (EtBr fluorescence emission) |
| Block band | UV (302 nm midrange) and excitation-source background wavelengths |
| Compatible imaging enclosure | E5001-SD SmartDoc 2.0 (and E5001-SDB bundled package) |
| Workflow context | SmartDoc + UV transilluminator + ethidium bromide stain |
| Sister filter | E5001-535 (535 nm for green stains on UV transilluminator) |
| Standard filter (included with SmartDoc) | E5001-ORANGE (for blue-light transilluminator workflow) |
Why a 590 nm Filter for EtBr Imaging
Ethidium bromide intercalates between DNA base pairs and fluoresces under UV excitation (302 nm midrange UV transilluminators are the standard excitation source). EtBr's emission peak is centered at ~590 nm (red-orange spectrum) — distinctly different from green fluorescent stains' ~525–540 nm emission peaks.
The E5001-590 band-pass filter is engineered to:
- Block 302 nm UV excitation — so the smartphone camera is protected and the captured image is UV-bulb-free
- Pass 590 nm EtBr emission with optimal signal-to-background ratio
- Block green-emission wavelengths — preventing cross-contamination between EtBr signal and any residual green-stain background
For labs running both EtBr and green-stain workflows on the same UV transilluminator, the E5001-535 + E5001-590 filter pair supports the alternating-workflow imaging protocol (swap filters per stain chemistry, image both workflows on the same SmartDoc + UV equipment).
SmartDoc Filter Selection Decision Matrix
| Workflow | Transilluminator | Stain | SmartDoc filter |
| Blue-light + green stain (default) | E4000 SmartBlue | SmartGlow / SYBR Green / Gel Green | E5001-ORANGE (included with SmartDoc) |
| UV + green stain | E3000 / E3100 / E3200 | SmartGlow / SYBR Green / Gel Green | E5001-535 |
| UV + EtBr (this filter applies) | E3000 / E3100 / E3200 | Ethidium bromide | E5001-590 (this product) |
| UV + Gel Red | E3000 / E3100 / E3200 | Gel Red (red-emission stain) | E5001-590 (Gel Red emission ~595 nm — close enough for the 590 nm pass-band) |
Application Profile
Labs running EtBr workflows on UV transilluminators — the classic and still widely-used DNA-band visualization workflow. The E5001-590 supports SmartDoc-based smartphone-camera documentation of these workflows.
Multi-stain imaging facilities — facilities running both EtBr and green-stain workflows alternate between E5001-590 (EtBr) and E5001-535 (green-stain) per imaging session.
Legacy lab workflows in transition — labs in the process of transitioning from EtBr to green stains may use the E5001-590 to maintain documentation continuity for in-progress EtBr workflows while standing up the green-stain alternative.
Educational laboratory historical-context workflows — teaching labs that demonstrate the EtBr workflow as part of molecular-biology coursework (showing students the historical standard alongside the modern green-stain alternative) maintain the EtBr filter for those exercises.
Forensic / clinical-research labs — facilities running EtBr in regulated workflows where the historical EtBr documentation chain must remain consistent benefit from the E5001-590 for SmartDoc-based modern documentation of legacy-protocol gels.
Compatible Stains
Per SmartDoc 2.0 manual, the E5001-590 is the appropriate filter for:
- Ethidium bromide (EtBr) — primary use case, ~590 nm emission peak
- Gel Red — red-emission DNA-binding stain with ~595 nm emission (within the 590 nm pass-band tolerance)
Ordering & Configuration
Pair with SmartDoc 2.0 + UV transilluminator + EtBr workflow inventory. The filter is workflow-required for the specific SmartDoc + UV + EtBr combination, not workflow-mandatory for all SmartDoc workflows. Confirm stain chemistry at order.
Sister-filter pairing for mixed workflows. Labs running both EtBr and green-stain workflows benefit from ordering the E5001-535 alongside the E5001-590 to support both stain chemistries on the same SmartDoc + UV equipment.
Confirm at quote: SmartDoc 2.0 chassis (E5001-SD / E5001-SDB) in inventory, UV transilluminator inventory, gel-stain chemistry (EtBr / Gel Red workflows), sister-filter inventory (E5001-535 for green-stain workflows), and lead time.
Warranty: Per Accuris standard accessory terms — confirm at quote.
SmartDoc 2.0 photo-filter family:
- E5001-ORANGE — Orange filter for blue-light + green-stain workflow (included with SmartDoc 2.0)
- E5001-535 — 535 nm band-pass for UV + green-stain workflow
- E5001-590 (this product) — 590 nm band-pass for UV + EtBr workflow
- E5001-UVBLOCK — UV blocking filter optimized for E3100 myView Compact UV
Pick the filter matching the transilluminator + stain combination in use; sister-filter pairing supports multi-stain facilities.
