The MS1000-PCR1 is the optically clear, peelable sealing film for qPCR plates — sold as pack of 100 seal films for use with the Accuris SmartSeal MS1000 Semi-Automated Plate Sealer. The film's optical clarity allows qPCR fluorescence excitation light to reach the wells and emission light to reach the qPCR reader's detector during thermal cycling, enabling real-time qPCR readout through the seal. The peelable design supports post-qPCR plate handling (sample aliquoting, downstream analysis) without leaving residue on the plate edge.
Per SmartSeal brochure recommended setting for clear seals: 170 °C, 2.5 seconds (starting parameters; actual settings should be tuned per workflow plate-seal combination).
Key Specifications
| SKU | MS1000-PCR1 |
| Type | Optically clear, peelable sealing film for qPCR |
| Pack quantity | 100 seal films per pack |
| Plate compatibility | Most PCR plate types (skirted / semi-skirted / non-skirted 96-well PCR plates) |
| Compatible chassis | MS1000 (115 V) SmartSeal Semi-Automated Plate Sealer |
| Optical property | Clear — transmits fluorescence excitation and emission for qPCR read-through |
| Recommended sealing parameters | 170 °C, 2.5 seconds (per SmartSeal brochure for clear seals) |
| Removal | Peelable |
| Workflow | qPCR (real-time PCR) — sealing during thermal cycling |
| List price | $166 (pack of 100) |
Compatibility & When to Order This SKU
qPCR clear seal consumable — SmartSeal chassis only. Order this SKU when:
- qPCR (real-time PCR) workflows — the optical-clear film transmits fluorescence excitation and emission, enabling real-time qPCR readout through the seal without seal-removal mid-cycle.
- PCR-plate sealing for thermal cycling protection — the seal prevents evaporation during the heating / cooling cycles of qPCR; well-volume integrity matters for accurate Ct value calculation.
- High-throughput qPCR facilities — labs running daily qPCR plate sealing benefit from steady consumable replenishment.
- Multi-format qPCR plate workflows — labs running mixed plate formats (skirted / semi-skirted / non-skirted PCR plates) benefit from the seal's multi-plate-type compatibility.
- Clinical-research diagnostic-development qPCR — diagnostic-development workflows running qPCR-based assays benefit from the optical-clear seal's reproducibility.
qPCR Plate Sealing — Why Optical Clarity Matters
qPCR thermal cycling has two stages where seal optical properties matter:
| Stage | Seal optical requirement |
| Thermal cycling | Heating / cooling cycles drive air expansion / contraction in the well headspace; the seal must withstand the pressure differential without leaking |
| Real-time fluorescence read | Excitation light from the qPCR reader's optics passes through the seal into the well; emission fluorescence passes back out through the seal to the detector |
Without optical-clear film, standard foil seals block fluorescence — qPCR cannot read through them — and removing seals before reading wastes qPCR throughput, introduces evaporation losses, and risks cross-well contamination.
With optical-clear film (this SKU), the seal stays on through the entire thermal cycling protocol, fluorescence excitation and emission pass through cleanly, qPCR Ct values stay accurate because well-volume integrity is preserved through cycling, and post-qPCR plate handling is easier thanks to the peelable design.
Recommended Sealing Setting
Per SmartSeal brochure: 170 °C, 2.5 seconds for "Most Clear-Seals" (the recommended starting setting for clear sealing films like MS1000-PCR1). Actual settings may need adjustment per:
- PCR plate brand and material — different PCR plate manufacturers may have slightly different surface chemistry
- PCR plate skirting — fully skirted vs semi-skirted vs non-skirted plates may seal slightly differently
- Plate temperature at seal time — pre-cooled plates may require slightly longer seal time
Tune within the chassis's 80–200 °C / 0.5–10 s range.
MS1000-PCR Family Selection
| Seal SKU | Type | Use case |
| MS1000-PCR1 (this product) | Optically clear, peelable | Standard qPCR with real-time fluorescence readout |
| MS1000-PCR2 | Pierce-seal for PCR | PCR workflows requiring needle-pierce access after sealing (automated post-PCR pipetting workflows) |
| MS1000-PCR3 | (additional PCR seal format per universe) | Per workflow needs |
Pick the MS1000-PCR1 (this product) for: standard qPCR workflows with real-time fluorescence readout — the workhorse qPCR seal.
Step to MS1000-PCR2 (pierce-seal) for workflows requiring automated post-PCR pipetting (e.g., qPCR followed by automated sample-transfer for downstream analysis without operator-removal of the seal).
Application Profile
Real-time qPCR workflows — the workhorse application. SYBR Green qPCR, TaqMan probe qPCR, multiplex qPCR — all benefit from the optical-clear seal for read-through fluorescence detection.
Clinical-research diagnostic-development qPCR — diagnostic-development workflows running qPCR-based pathogen detection, gene-expression quantification, or genotyping benefit from the seal's reproducibility.
RT-qPCR workflows — RT-qPCR runs (with One-Step or Two-Step RT) benefit from the clear seal for the qPCR readout phase.
High-throughput qPCR facilities — sequencing cores and clinical-research labs running multi-plate-per-day qPCR batches consume MS1000-PCR1 at a steady cadence.
Method-development qPCR runs — protocol-validation qPCR runs benefit from consistent sealing for run-to-run reproducibility comparison.
Multi-investigator shared qPCR equipment — shared qPCR equipment in core facilities benefits from the standardized clear-seal consumable.
Ordering & Configuration
Per-plate workflow consumable. Each qPCR plate-sealing cycle consumes one seal film; pack of 100 films supports 100 qPCR plate-sealing cycles before reorder.
qPCR plate format check at order. Confirm the lab's qPCR plate format — the MS1000-PCR1 supports most PCR plate types but specific plate-brand compatibility should be verified at first use.
Confirm at quote: chassis pairing (MS1000 in inventory), qPCR plate format, qPCR reader compatibility (the seal must transmit the reader's excitation / emission wavelengths — typically 470–650 nm for most qPCR fluorophores), and lead time.
Warranty: Per Accuris standard consumable terms — confirm at quote.
Accuris SmartSeal qPCR seal-film family:
- MS1000-PCR1 (this product) — Optically clear, peelable, for qPCR, 100/pk ($166)
- MS1000-PCR2 — Pierce-seal for PCR (post-PCR pipetting workflows)
- MS1000-PCR3 — Additional PCR seal format
Pick the optically clear seal for standard real-time qPCR; step to pierce-seal for automated post-PCR pipetting workflows.
