Accuris ssDNA Quantification Kit, 100 Assays

The F1000-SD-100 is the 100-assay Accuris ssDNA Quantification Kit, the single-stranded DNA-specific chemistry in the F1000 fluorometric family. Distinct from the dsDNA-binding chemistry of the F1000-BR / F1000-HS variants (which don't reliably detect single-stranded DNA), the F1000-SD uses a chemistry that binds ssDNA specifically, useful for sequencing template prep (cycle-sequencing extension products are single-stranded), oligonucleotide quantification, denatured-DNA workflows, and any application where the target nucleic acid is single-stranded.

Key Specifications

For full kit chemistry framework and Smart-Q family integration, see the F1000-BR-100 parent page (F1000 family conventions).

Why ssDNA-Specific Chemistry

dsDNA-binding chemistry (Qubit BR / HS, F1000-BR / F1000-HS) binds the dsDNA major groove and produces fluorescence proportional to dsDNA concentration. Single-stranded DNA doesn't have the dsDNA major-groove geometry. dsDNA-binding chemistry produces little or no signal on ssDNA samples, undercounting or missing the ssDNA entirely.

The F1000-SD uses an ssDNA-binding dye that detects single-stranded DNA specifically. This matters for:

• Cycle-sequencing extension product quantification. Sanger cycle-sequencing produces ssDNA extension products that need quantification before sequencer loading
• Oligonucleotide quantification. Synthetic oligonucleotides (PCR primers, hybridization probes, antisense oligos) ship as ssDNA and require ssDNA-specific quantification
• Denatured DNA workflows. DNA samples that have been heat-denatured or chemical-denatured to single-stranded form
• Bisulfite-converted DNA. Methylation-analysis workflows where bisulfite conversion produces single-stranded DNA
• Phage display library quantification. M13 / fd phage libraries circulate as single-stranded DNA in their phage form
• Large-genome library prep ssDNA intermediates. Some NGS library prep protocols include ssDNA intermediates that need QC quantification

Application Profile

Sanger cycle-sequencing template QC. Quantifying ssDNA extension products before sequencer loading.

Oligonucleotide-purification QC. Confirming oligo concentration after HPLC purification or PAGE purification.

Antisense oligonucleotide therapeutics development. Quantifying therapeutic ssDNA constructs in pharma R&D workflows.

Bisulfite methylation-analysis workflows. Quantifying bisulfite-converted ssDNA before downstream methylation amplification.

Phage display library QC. Quantifying M13 / fd phage display library ssDNA.

Specialty NGS library prep workflows. Library prep protocols that include ssDNA intermediates requiring QC quantification.

Ordering & Configuration

Storage & handling. Per kit instructions: typical fluorometric kit storage at 4 °C protected from light.

Confirm at quote: Smart-Q chassis pairing, ssDNA workflow context, expected workflow throughput, shipping conditions (cold-chain), and lead time.

Warranty: Per Accuris Life Science Reagents standard kit warranty terms. Confirm at quote.

F1000 nucleic acid quantification kit family:

- F1000-BR / F1000-HS: dsDNA chemistry (Broad Range / High Sensitivity)

- F1000-RBR / F1000-RHS: RNA chemistry (Broad Range / High Sensitivity)

- F1000-SD-100 (this product): ssDNA chemistry, 100 assays

- F1000-SD-500: ssDNA chemistry, 500 assays (institutional bulk)

Pick the F1000-SD when the target nucleic acid is single-stranded. The dsDNA / RNA chemistry kits don't reliably detect ssDNA.