The F1000-BR-100 is the 100-assay Accuris Broad Range dsDNA Quantification Kit — a fluorometric quantification reagent kit for the Accuris Smart-Q fluorometer family (F0100 Mini, F1001 Smart-Q 100, F1008 Smart-Q 800). The Broad Range (BR) chemistry covers typical dsDNA concentration ranges (typically 0.2 ng/µL to 100 ng/µL input — similar to Qubit BR chemistry), useful for routine post-extraction quantification, NGS library prep concentration measurement, and general dsDNA workflows. Standard concentrate variant — operator dilutes to working concentration before each assay batch; the 1X pre-diluted ready-to-use variant is F1000-BR1-100 (slightly higher per-assay cost for the convenience).
Key Specifications
| SKU | F1000-BR-100 |
| Type | Fluorometric quantification kit — Broad Range dsDNA |
| Pack quantity | 100 assays |
| Assay range | Broad Range (BR) — typically 0.2–100 ng/µL dsDNA input range |
| Format | Standard concentrate (operator dilutes to working concentration) |
| 1X pre-diluted variant | F1000-BR1-100 |
| Per-assay cost | ~$1.35 |
| Compatible chassis | F0100 Smart-Q Mini, F1001 Smart-Q 100, F1008 Smart-Q 800 (and -E voltage variants) |
| List price | $135 |
F1000 Family Position — Broad Range vs High Sensitivity, dsDNA vs RNA vs ssDNA
The Accuris F1000 fluorometric quantification kit family ships in multiple chemistries for different sample types and concentration ranges:
| Kit family | Sample type | Range | When to pick |
| F1000-BR (this kit's family) | dsDNA | Broad Range (~0.2–100 ng/µL) | Routine dsDNA quantification post-extraction, NGS library QC |
| F1000-HS | dsDNA | High Sensitivity (low concentration) | Low-input samples (FFPE DNA, low-yield extractions, cfDNA) |
| F1000-RBR | RNA | Broad Range | Routine RNA quantification post-extraction |
| F1000-RHS | RNA | High Sensitivity | Low-input RNA (cell-free RNA, low-yield extractions) |
| F1000-SD | ssDNA | (single concentration tier) | Single-stranded DNA workflows (sequencing template prep, oligo quantification) |
Each chemistry ships in standard concentrate and 1X pre-diluted variants (1X variants suffix the number with 1: BR1, HS1) at 100-assay and 500-assay pack sizes.
Standard Concentrate vs 1X Pre-Diluted
| Variant | Format | Workflow |
| F1000-BR-100 (this product) | Standard concentrate | Operator dilutes the working solution per assay batch (mix concentrate with assay buffer per kit instructions) |
| F1000-BR1-100 | 1X pre-diluted ready-to-use | Working solution shipped pre-diluted; eliminates the dilution step at the bench (reduced workflow time, reduced operator-dilution variability) |
Pick the standard concentrate (F1000-BR-100) when the lab's workflow doesn't benefit from the pre-diluted convenience and per-assay cost matters. Pick the 1X (F1000-BR1-100) when workflow time and dilution-step elimination matter.
Application Profile
Routine post-extraction dsDNA quantification — clinical-research, academic, and core-facility labs running dsDNA quantification after DNA extraction.
NGS library prep concentration measurement — quantification before pooling for sequencing run normalization in the broad-range concentration window.
Routine PCR template quantification — accurate template quantification for PCR setup where dsDNA concentration matters.
Educational laboratory teaching workflows — teaching molecular biology curricula where fluorometric quantification methodology is part of the curriculum.
Cross-Smart-Q-chassis workflows — labs running multiple Smart-Q chassis (Mini, 100, 800) benefit from a single F1000-BR kit covering all chassis workflows.
Ordering & Configuration
Storage & handling. Per kit instructions — typical fluorometric kit storage at 4 °C protected from light. Confirm storage and handling requirements at receipt per kit instructions.
Working dilution preparation. This standard concentrate kit requires operator dilution to working concentration per the kit instructions before each assay batch. The 1X variant (F1000-BR1-100) eliminates this step.
Confirm at quote: Smart-Q chassis pairing (F0100 / F1001 / F1008 in inventory), assay-format preference (standard concentrate vs 1X pre-diluted), expected workflow throughput (100-assay pack vs 500-assay larger pack), shipping conditions (cold-chain), and lead time.
Warranty: Per Accuris Life Science Reagents standard kit warranty terms — confirm at quote.
F1000 Broad Range dsDNA — pack and format variants:
- F1000-BR-100 (this product) — 100 assays, standard concentrate
- F1000-BR-500 — 500 assays, standard concentrate
- F1000-BR1-100 — 100 assays, 1X pre-diluted
- F1000-BR1-500 — 500 assays, 1X pre-diluted
For low-concentration / low-input dsDNA, see F1000-HS family. For RNA quantification, see F1000-RBR / F1000-RHS sister families.
