The F1000-HS-100 is the 100-assay Accuris High Sensitivity dsDNA Quantification Kit — the low-concentration variant of the F1000 dsDNA quantification family. The High Sensitivity (HS) chemistry covers low-input dsDNA concentration ranges (typically 0.005–10 ng/µL — substantially lower than the Broad Range chemistry at 0.2–100 ng/µL), useful for low-yield extraction workflows (FFPE DNA, low-input clinical samples), cfDNA / liquid biopsy sample quantification, and any workflow where target dsDNA concentration falls below the BR detection range.
Key Specifications
For full kit chemistry framework, application context, and Smart-Q family integration, see the F1000-BR-100 parent page (which establishes the F1000 family conventions). This page documents the High Sensitivity variant at the 100-assay pack size.
| SKU | F1000-HS-100 |
| Type | Fluorometric quantification kit — High Sensitivity dsDNA |
| Pack quantity | 100 assays |
| Assay range | High Sensitivity — typically 0.005–10 ng/µL dsDNA input range |
| Format | Standard concentrate (operator dilutes to working concentration) |
| 1X pre-diluted variant | F1000-HS1-100 |
| Per-assay cost | ~$1.35 |
| Compatible chassis | F0100 Smart-Q Mini, F1001 Smart-Q 100, F1008 Smart-Q 800 |
| List price | $135 |
Broad Range vs High Sensitivity — Chemistry Selection
| Chemistry | Concentration range | When to pick |
| F1000-BR Broad Range | 0.2–100 ng/µL | Routine quantification post-extraction (most workflows) |
| F1000-HS (this kit's family) | 0.005–10 ng/µL (low-input) | Low-yield extractions (FFPE, cfDNA, liquid biopsy), NGS library QC for low-input prep, single-cell sequencing prep |
If the workflow's expected dsDNA concentration is unknown or spans both ranges, pick the BR for typical workflows or the HS for low-input workflows. Some labs run both kits in parallel for samples that may fall in either range.
Application Profile
Low-input dsDNA quantification post-extraction — clinical-research and pharma R&D labs running extraction on low-yield sample types (FFPE tissue, microbiome low-biomass samples, ancient DNA prep).
cfDNA / liquid biopsy quantification — clinical-research and translational research workflows quantifying circulating cell-free DNA from plasma or serum.
NGS library QC for low-input library prep — quantification before pooling for sequencing run normalization where low-input library prep produces concentrations below the BR detection range.
Single-cell sequencing prep — single-cell genomics workflows where individual-cell DNA prep produces sub-ng/µL concentrations.
Low-yield amplification product quantification — quantification of low-input PCR products or sub-ng amplicon prep.
Ordering & Configuration
Storage & handling. Per kit instructions — typical fluorometric kit storage at 4 °C protected from light.
Confirm at quote: Smart-Q chassis pairing, assay-format preference (standard concentrate vs 1X pre-diluted), expected workflow throughput, shipping conditions (cold-chain), and lead time.
Warranty: Per Accuris Life Science Reagents standard kit warranty terms — confirm at quote.
F1000 High Sensitivity dsDNA — pack and format variants:
- F1000-HS-100 (this product) — 100 assays, standard concentrate
- F1000-HS-500 — 500 assays, standard concentrate
- F1000-HS1-100 — 100 assays, 1X pre-diluted
- F1000-HS1-500 — 500 assays, 1X pre-diluted
For routine concentration ranges (0.2-100 ng/µL), see F1000-BR family. For RNA quantification, see F1000-RHS sister family.
