
2 × 250 mL Bottle Insert for Z496-1000-RBUK (2/pk)
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The Z496-1000-A250 is the 2 × 250 mL bottle insert for the Hermle Z496-1000-RBUK rectangular bucket — sold as a 2-pack. Each insert holds 2 standard 250 mL polypropylene centrifuge bottles (Hermle C1250 SKU — same-geometry third-party 250 mL bottles may also fit; verify dimensions) per bucket position. With the full 4-bucket configuration (2 units of this SKU = 4 inserts), the rotor processes 8 × 250 mL bottles per cycle — meaningful throughput for mid-volume bottle workflows on the Z496-K chassis.
| SKU | Z496-1000-A250 |
| Type | Bottle insert — 2 × 250 mL per insert |
| Pack quantity | 2 inserts per SKU (full 4-bucket configuration requires 2 units) |
| Per-cycle throughput (full configuration) | 8 × 250 mL bottles per cycle (2 bottles × 4 buckets) |
| Bottle format | 250 mL polypropylene centrifuge bottle (Hermle C1250 — verify dimensions if using third-party 250 mL bottles) |
| Compatible bucket | Z496-1000-RBUK (rectangular bucket for Z496-1000-AC rotor body) |
| Compatible chassis (assembled) | Z496-K (Floor-Standing, 230 V) and Z496-K-UC (Underbench, 230 V) |
| Max. operating speed (with insert + bottles) | Per Z496-1000-RBUK spec — 4,200 rpm at 3,964 × g maximum |
| List price | $1,052 (2-pack) |
Insert pairing — Z496-1000-RBUK rectangular bucket only. Engineered to fit the rectangular-bucket envelope. Not compatible with round buckets (Z496-1000-1L) or with bucket geometries on other Hermle chassis families.
Bottle pairing — C1250 (250 mL). The 250 mL polypropylene centrifuge bottle (Hermle C1250 SKU) is the engineered bottle fit. Each insert holds 2 bottles side-by-side per bucket position.
Throughput vs single-bottle inserts. The Z496-1000-A250 doubles bottle throughput per bucket vs the single-bottle Z496-1000-A500 insert (8 × 250 mL per cycle vs 4 × 500 mL). Pick based on whether the workflow's sample-volume profile fits 250 mL or 500 mL formats.
Mid-volume bottle workflows at maximum throughput on the Z496-K — 8 × 250 mL per cycle handles batch cell-culture pellet recovery, vaccine pre-concentration, and recombinant-protein lysate clarification at meaningful per-cycle throughput.
Cell-culture pellet recovery from 250 mL aliquots — when the workflow batches 250 mL spinner-flask or wave-bag aliquots for clarification.
Mixed-format flexibility. Switch between insert types (Z496-1000-A500 / -A250 / -A225) on the same rectangular bucket to handle different bottle formats — useful for facilities running multi-volume workflows on the same chassis.
Cold-chain bottle workflows on Z496-K — combines per-cycle throughput with chassis refrigeration for cold-stable protein and cell-culture work at 250 mL bottle scale.
Insert installation. Inserts seat into Z496-1000-RBUK bucket per Hermle service instructions. Confirm seat integrity before loading bottles.
8-bottle symmetric loading. With 2 bottles per bucket × 4 buckets, balance considerations require: (a) all 4 buckets with the same insert configuration, (b) bottles within each bucket paired by mass, and (c) bucket pairs across the rotor balanced by total mass.
Z496-1000-A250 ships as 2 inserts. Bottles (C1250), bucket (Z496-1000-RBUK), and rotor body (Z496-1000-AC) sold separately.
Confirm rectangular-bucket inventory, rotor-body pairing, bottle source, and insert count at quote.
Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts.
Insert family selection: the Z496-1000-A250 (this product, 2 × 250 mL per insert) is the throughput pick for 250 mL bottle workflows. For 500 mL bottles, see Z496-1000-A500. For 225 mL conical bottles, see Z496-1000-A225.
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