The Z496-1000-A225 is the 225 mL conical bottle insert for the Hermle Z496-1000-RBUK rectangular bucket — sold as a 2-pack. Engineered for conical-bottom centrifuge bottles in the 175–225 mL range (compatible with Hermle C0225 225 mL bottle and C0175 175 mL bottle), this insert holds the conical bottle securely at the correct centrifugation orientation for swing-out separation. Conical-bottom geometry is preferred for workflows where pellet recovery via aspiration to the conical tip matters (cell-culture pellets, fine-particulate sediment recovery).
Key Specifications
| SKU | Z496-1000-A225 |
| Type | Conical bottle insert — for rectangular bucket |
| Pack quantity | 2 inserts per SKU (full 4-bucket configuration requires 2 units) |
| Bottle format | 225 mL conical (Hermle C0225) or 175 mL conical (Hermle C0175) — same insert geometry accepts both |
| Compatible bucket | Z496-1000-RBUK (rectangular bucket for Z496-1000-AC rotor body) |
| Compatible chassis (assembled) | Z496-K (Floor-Standing, 230 V) and Z496-K-UC (Underbench, 230 V) |
| Max. operating speed (with insert + bottle) | Per Z496-1000-RBUK spec — 4,200 rpm at 3,964 × g maximum |
| Bottle bottom geometry | Conical (vs round-bottom inserts elsewhere in the family) |
| List price | $1,052 (2-pack) |
Compatibility & Configuration
Insert pairing — Z496-1000-RBUK rectangular bucket only. Engineered to fit the rectangular-bucket envelope. Not compatible with round buckets or with bucket geometries on other Hermle chassis families.
Bottle pairing — C0225 (225 mL) or C0175 (175 mL). Both Hermle conical centrifuge bottles fit the same insert geometry; pick the bottle volume that matches your sample-volume profile.
Conical-bottom geometry advantage. Conical-bottom centrifuge bottles concentrate the pellet at the conical tip, easing pellet recovery via pipette aspiration after the spin. Round-bottom inserts (Z496-1000-A500, Z496-1000-A250) produce a wider pellet distribution against the round bottom — appropriate for some workflows but not for fine-pellet recovery where the conical tip-concentration matters.
Application Profile
Cell-culture pellet recovery in 225 mL conical — the conical bottom concentrates the cell pellet for clean aspiration of the supernatant and easy resuspension of the pellet in a small volume.
Sediment recovery from environmental samples — fine-particulate environmental samples (water, sediment, biomass) benefit from conical-bottom geometry for recovery of the concentrated solid phase.
Fine-particulate slurry processing in 225 mL or 175 mL conical format on the Z496-K rotor.
Mid-volume protein-pellet recovery — when the workflow's sample volume sits between 175 mL and 225 mL and conical-bottom pellet concentration matters.
Cold-chain conical-bottle workflows on Z496-K — combines conical-pellet geometry with chassis refrigeration for cold-stable cell-culture and protein workflows.
Ordering & Configuration
Insert installation. Inserts seat into Z496-1000-RBUK bucket per Hermle service instructions.
Symmetric loading. All 4 buckets must use matching insert configurations; bottles paired by mass across opposing buckets.
Z496-1000-A225 ships as 2 inserts. Bottles (Hermle C0225 or C0175), Z496-1000-RBUK rectangular bucket, and Z496-1000-AC rotor body sold separately.
Confirm rectangular-bucket inventory, rotor-body pairing, bottle format choice (C0225 or C0175), and insert count at quote.
Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts.
Insert family selection:
- Z496-1000-A500 — 500 mL bottle insert (uses C1500 bottle)
- Z496-1000-A250 — 2 × 250 mL bottle insert (uses C1250 bottles)
- Z496-1000-A225 (this product) — 225 mL conical insert (uses C0225 / C0175 bottles)
Pick the conical-bottom insert (this SKU) when pellet-tip concentration matters for downstream pellet recovery.