The Z366-06250-A50 is the 50 mL Falcon conical tube adapter for the Hermle Z366-06250 6 × 250 mL angle rotor on the Z366 / Z366-K chassis — sold as a 2-pack. Each adapter holds 1 × 50 mL conical tube; the 6-position rotor takes 3 packs (6 adapters) for full symmetric loading, delivering 6 × 50 mL conical tubes per cycle (300 mL total) at the host rotor's high-RCF range (10,016 × g). Sister adapter to the A50R (50 mL round-bottom) — same 50 mL volume but different tube envelope geometry.
Key Specifications
| SKU | Z366-06250-A50 |
| Type | Tube adapter — 250 mL angle position → 1 × 50 mL conical |
| Pack quantity | 2 adapters per SKU (full 6-position rotor takes 3 units = 6 adapters) |
| Per-adapter capacity | 1 × 50 mL conical tube |
| Per-cycle throughput (full configuration) | 6 × 50 mL conical = 300 mL total per cycle |
| Tube format | 50 mL conical Falcon-style (29 mm OD, 118 mm length) |
| Compatible host rotor | Z366-06250 (6 × 250 mL angle rotor) |
| Compatible chassis | Z366, Z366-K (refrigerated), and -E voltage variants |
| Max. operating speed (with adapter + tube) | Per Z366-06250 spec — 8,000 rpm at 10,016 × g maximum |
| Max. radius | 14 cm |
| Temperature at max speed (refrigerated chassis) | 3 °C |
| Angle | 28° (fixed-angle host rotor) |
| List price | $275 (2-pack) |
Compatibility & When to Order This SKU
Adapter pairing — Z366-06250 250 mL angle rotor only. Order this SKU when:
- Adding 50 mL Falcon conical workflow to a Z366-06250 install — order 3 units for full 6-position symmetric loading (6 tubes/cycle).
- High-RCF cold-chain 50 mL conical prep — when the workflow needs the >10,000 × g range for 50 mL conical tubes at refrigerated temperature.
- Mixed 250 / 50 mL workflow consolidation — when the chassis primarily runs 250 mL bottles but occasional 50 mL conical workflows arise.
- Replacement adapters if a current set is damaged or worn.
Why High-RCF 50 mL Conical Matters
The Z366-06250-A50 + Z366-06250 + Z366-K refrigerated chassis combination delivers a configuration not common in the broader centrifuge market: 50 mL conical workflow at >10,000 × g with 3 °C cold-chain integrity. Most dedicated 50 mL angle rotors top out at 4,000–8,000 × g; the 250 mL host rotor's higher max speed pushes 50 mL workflow into the high-RCF range typically reserved for smaller-volume tubes.
This matters when:
- Cell-pellet recovery in 50 mL conical needs tight pellet compaction at high RCF
- Density-gradient prep in 50 mL conical needs >10,000 × g for clean phase separation
- Cold-chain preserves protein integrity, gradient stability, or temperature-sensitive analytes through a high-force spin step
The 6-tube-per-cycle throughput is lower than dedicated 50 mL rotors (Z326-1050 at 10 × 50 mL, Z206-0650 at 6 × 50 mL), but the trade-off is justified when the workflow specifically needs the high-RCF range.
Application Profile
High-RCF cold-chain 50 mL cell-pellet recovery — pelleting cells from 50 mL suspension culture batches at high RCF and refrigerated temperature for downstream-stable preparation.
High-RCF 50 mL conical density-gradient prep — Ficoll, Percoll, sucrose-gradient prep at 6 × 50 mL parallel preps at >10,000 × g.
Cold-chain protein-prep workflows in 50 mL conical — TCA, ammonium-sulfate, organic-precipitation in 50 mL working volume at high RCF on the refrigerated chassis.
Clinical-research and biobank prep at 50 mL high-RCF cold-chain — patient sample fractionation, aliquot prep, freeze-storage workflows where the >10,000 × g range matters for the workflow output.
Mixed 250 / 50 mL workflow consolidation — when the lab's primary chassis is the Z366-06250 for 250 mL bottle workflows and 50 mL conical workflows are part of the protocol.
Cross-format Z366-K core facilities — shared cores running mixed bottle and tube workflows on the cold-chain high-RCF chassis.
Ordering & Configuration
6-position symmetric loading. All 6 positions must use matching A50 adapters for symmetric balance at 8,000 rpm.
Adapter seat verification. Confirm each adapter is fully seated in its 250 mL position before loading the 50 mL conical tube.
Tube cap confirmation. All 50 mL conical tubes must be securely capped before spinning at 8,000 rpm — at the host rotor's high RCF, cap integrity is critical.
Z366-06250-A50 ships as 2 adapters. 50 mL conical tubes are operator-supplied (commodity Falcon / Corning / equivalent formats).
Confirm at quote: host rotor (Z366-06250 in inventory), chassis pairing (Z366, Z366-K), tube format (50 mL conical Falcon-style; round-bottom uses A50R), pack count (3 packs for full 6-position configuration), and lead time.
Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the adapters.
Z366-06250 50 mL adapter pair:
- Z366-06250-A50 (this product) — 50 mL Falcon conical
- Z366-06250-A50R — 50 mL round-bottom (different tube envelope)
Match the adapter to the workflow's tube envelope. Both deliver 6-tube parallel throughput on the full 6-position configuration with the host rotor's high-RCF cold-chain capability.
