The Z326-100H-A85 is the 85 mL round-bottom glass bottle insert for the Hermle Z326-100H sealed bucket on the Z327 / Z327-K chassis 4-place swing-out rotor — sold as a 2-pack. Each insert holds 1 × 85 mL bottle; the 4-bucket rotor configuration takes 2 packs (4 inserts) for full symmetric loading, delivering 4 × 85 mL bottles per cycle (340 mL total per cycle) in hermetically sealed swing-out geometry on a refrigerated chassis. The 85 mL format sits in the mid-volume bottle workflow range — between the 50 mL conical/round inserts (A50, A50R) and the larger 100 mL bottle insert (A100).
Key Specifications
| SKU | Z326-100H-A85 |
| Type | Bottle insert — 85 mL round-bottom glass |
| Pack quantity | 2 inserts per SKU (full 4-bucket configuration takes 2 units = 4 inserts) |
| Per-insert capacity | 1 × 85 mL bottle |
| Per-cycle throughput (full configuration) | 4 × 85 mL = 340 mL total per cycle |
| Bottle format | 85 mL round-bottom glass (38 mm OD, 90–108 mm length) |
| Compatible bucket | Z326-100H sealed bucket (with Z326-100H-LID for hermetic seal) |
| Compatible chassis | Z327, Z327-K (refrigerated), and -E voltage variants |
| Max. operating speed (with insert + bottle) | Per Z326-100H spec — 5,000 rpm at 4,080 × g maximum |
| Max. radius | 14.6 cm (0.6 cm radius correction with this insert) |
| Temperature at max speed (Z327-K refrigerated) | 2 °C |
| Geometry | Swing-out (0–90°) — flat sediment surface |
| List price | $267 (2-pack) |
Compatibility & When to Order This SKU
Insert pairing — Z326-100H sealed bucket only. Order this SKU when:
- Adding 85 mL bottle workflow to an existing Z327 / Z327-K + Z326-100H install — order 2 units for full 4-bucket symmetric loading (340 mL total per cycle).
- Cold-chain mid-volume bioprocess prep — vaccine-development, recombinant-protein, and cell-culture workflows that batch 85 mL bottles with cold-chain integrity.
- BSL-2 + 85 mL bottle workflows — when 85 mL cell-culture aliquots require aerosol containment during centrifugation.
- Replacement inserts if a current set is damaged or worn.
Application Profile
Hermetically sealed cold-chain mid-volume bottle workflow. The Z326-100H bucket + Z326-100H-LID + A85 insert + Z327-K chassis combination delivers 85 mL bottle workflows with cold-chain integrity (2 °C at max speed), biosafety containment (sealed bucket envelope), and swing-out flat-sediment geometry.
Glass round-bottom bottle compatibility. The A85 envelope is dimensioned for the 38 mm OD × 90–108 mm length range — matches standard 85 mL round-bottom glass bottle formats. Bottles outside this dimensional range may not seat properly; verify dimensions if using non-standard bottle suppliers.
Mid-volume position in the bottle insert family. The A85 sits between the smaller A50 / A50R (50 mL) and the larger A100 (100 mL) bottle inserts — the right pick when 85 mL is the workflow's specific intermediate volume.
4-bucket symmetric loading. All 4 buckets must use matching A85 inserts for symmetric balance.
Design Architecture
Cold-chain mid-volume cell harvest workflows — pelleting cells from 85 mL suspension culture batches at refrigerated temperature for downstream-stable preparation.
Bioprocess intermediate prep at 85 mL cold-chain — vaccine-development and bioprocess workflows that use 85 mL bottles as the intermediate concentration step between smaller (50 mL) and larger (100 mL) volumes, with cold-chain integrity.
BSL-2 + bioprocess prep — recombinant-protein expression workflows handling genetically modified organisms in 85 mL bottle format that require aerosol containment.
Mid-volume institutional clinical-research prep — pooled patient sample workflows in 85 mL working volume on the cold-chain biosafety chassis.
Cold-chain protein-prep workflows — TCA, ammonium-sulfate, organic-precipitation in 85 mL working volume on the refrigerated biosafety chassis.
Cross-format Z327-K chassis flexibility — labs running 85 mL bottle alongside other tube and bottle formats benefit from stocking the cross-format Z326-100H insert family.
Ordering & Configuration
4-bucket symmetric loading. All 4 buckets must use matching A85 inserts. Mixed-bucket configurations produce mass asymmetry.
Hermetic-seal engagement. Per Hermle service instructions, the Z326-100H-LID must be fully engaged on each bucket before each spin to maintain the hermetic envelope at 4,080 × g.
Bottle cap confirmation. All bottles must be capped securely before spinning at 5,000 rpm.
Bottle volume balance. When the 4 bottles carry different sample volumes, pre-balance pairs by total mass before spinning.
Z326-100H-A85 ships as 2 inserts. Bottles (85 mL round-bottom glass) and the Z326-100H bucket and Z326-100H-LID are sold separately.
Confirm at quote: bucket pairing (Z326-100H + Z326-100H-LID in inventory), chassis pairing (Z327 or Z327-K), bottle format (85 mL round-bottom glass, 38 mm OD), workflow drivers (cold-chain, biosafety), pack count (2 packs for full 4-bucket configuration), and lead time.
Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts. Hermetic-seal interface is a planned-inspection item.
Z326-100H bottle-insert family (Z327 / Z327-K chassis):
- Z326-100H-A85 (this product) — 85 mL round-bottom glass (mid-volume)
- Z326-100H-A100 — 100 mL round-bottom glass (largest bottle insert in this family)
- Z326-100H-A50 / A50R — 50 mL conical / round (smaller mid-volume)
Pick the A85 when 85 mL bottle is the workflow's specific working volume — the mid-step between 50 mL and 100 mL bottle workflows.
