The Z446-750-B50 is a 7-position 50 mL conical tube insert for the Hermle Z446-MP-BUK rectangular bucket on the Z446-750-AC 4-place swing-out rotor (Z446 / Z446-K chassis) — sold as a 2-pack. Each insert holds 7 × 50 mL conical tubes; the 4-bucket rotor configuration takes 2 packs (4 inserts) for full symmetric loading, delivering 28 × 50 mL conical tubes per cycle (1.4 L total) in swing-out geometry on the rectangular-bucket platform.
Key Specifications
| SKU | Z446-750-B50 |
| Type | Tube insert — 7 × 50 mL conical (rectangular bucket) |
| Pack quantity | 2 inserts per SKU (full 4-bucket configuration takes 2 units = 4 inserts) |
| Per-insert capacity | 7 × 50 mL conical tubes |
| Per-cycle throughput (full configuration) | 28 × 50 mL conical = 1,400 mL total per cycle |
| Tube format | 50 mL conical Falcon-style (29 mm OD, 116 mm length) |
| Compatible bucket | Z446-MP-BUK (rectangular bucket on Z446-750-AC swing-out rotor) |
| Compatible rotor | Z446-750-AC (4-place swing-out rotor body) — rectangular bucket variant |
| Compatible chassis | Z446, Z446-K (refrigerated), and -E voltage variants |
| Max. operating speed (with insert + tubes) | Per Z446-MP-BUK spec — 4,000/4,500 rpm at 3,130–3,961 × g maximum |
| Max. radius | 17.5 cm (rectangular bucket geometry) |
| Temperature at max speed (refrigerated chassis) | 4 °C |
| Geometry | Swing-out (0–90°) — flat sediment surface |
| List price | $424 (2-pack) |
50 mL Inserts on the Z446-750-AC Rotor — Conical vs Round-Bottom
The Z446-750-AC swing-out rotor offers two 50 mL insert formats:
| Configuration | Per cycle | Bucket | Max RCF |
| Z446-750-B50 (this SKU) on rectangular bucket | 28 × 50 mL conical | Z446-MP-BUK | 3,961 × g |
| Z446-750-A50R on round bucket | 28 × 50 mL round-bottom | Z446-750-BUK | 4,346 × g |
The B50 (rectangular bucket) and A50R (round bucket round-tube) both deliver 28 tubes/cycle; the B50 supports conical tubes specifically on the rectangular bucket geometry. Pick B50 when the lab uses the rectangular bucket for mixed microplate / 50 mL conical workflows.
Compatibility & When to Order This SKU
Insert pairing — Z446-MP-BUK rectangular bucket only. Order this SKU when:
- Adding 50 mL conical workflow to a Z446-750-AC + Z446-MP-BUK rectangular-bucket install — order 2 units for full 4-bucket symmetric loading (28 tubes/cycle, 1.4 L total).
- Mixed microplate / 50 mL workflow consolidation — when the lab uses the rectangular bucket primarily for microplate workflows and the B50 insert adds 50 mL conical capability without round-bucket swap.
- Cold-chain 50 mL conical clinical-research and biobank prep on the rectangular-bucket platform.
- Replacement inserts if a current set is damaged or worn.
Application Profile
4-bucket symmetric loading. All 4 rectangular buckets must use matching B50 inserts for symmetric balance.
Per-insert symmetric loading. Within each insert, the 7 conical tubes should be balanced symmetrically — pair tubes by mass with the seventh position symmetric to the insert's central axis.
Design Architecture
Mixed microplate / 50 mL workflow consolidation — when the lab uses the Z446-MP-BUK rectangular bucket primarily for microplate workflows and the B50 insert adds 50 mL conical capability without round-bucket swap.
Cold-chain 50 mL conical clinical-research and biobank prep on the rectangular-bucket platform — patient sample fractionation, aliquot prep, freeze-storage workflows at 28 × 50 mL conical per cycle on the cold-chain swing-out chassis.
Cell-culture pellet recovery in 50 mL — pelleting cells from 28 parallel 50 mL conical suspension batches per cycle on the rectangular-bucket platform.
Density-gradient prep in 50 mL — Ficoll, Percoll, sucrose-gradient prep at 28 parallel preps per cycle.
Cold-chain protein prep in 50 mL conical — TCA, ammonium-sulfate, organic-precipitation in 50 mL working volume at 28 parallel preps with refrigeration.
Cross-format Z446-K core facilities — labs running both microplate workflows (rectangular bucket direct-fit) and 50 mL conical workflows (this B50 insert) on the same Z446-MP-BUK inventory benefit from workflow flexibility.
Ordering & Configuration
4-bucket symmetric loading. All 4 rectangular buckets must use matching B50 inserts. Mixed-bucket configurations produce mass asymmetry.
Tube cap confirmation. All 50 mL conical tubes must be securely capped before spinning at 4,500 rpm.
Z446-750-B50 ships as 2 inserts. 50 mL conical tubes are operator-supplied (commodity Falcon / Corning / equivalent formats). The Z446-MP-BUK rectangular bucket is sold separately.
Confirm at quote: bucket pairing (Z446-MP-BUK rectangular bucket in inventory — distinct from the round bucket Z446-750-BUK which uses A50R inserts), chassis pairing (Z446 or Z446-K) and power configuration, tube format (50 mL conical Falcon-style), pack count (2 packs for full 4-bucket configuration = 28 tubes/cycle), and lead time.
Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts.
Z446-750 50 mL workflow paths (Z446-750-AC swing-out rotor):
- Z446-750-B50 (this product) — 7 × 50 mL conical on Z446-MP-BUK rectangular bucket (28/cycle, 17.5 cm radius)
- Z446-750-A50R — 7 × 50 mL round on Z446-750-BUK round bucket (28/cycle, 19.2 cm radius)
Pick B50 for rectangular-bucket workflows with conical tubes; pick A50R for round-bucket round-tube workflows.
