The Z327-200-A50R is the 50 mL round-bottom glass tube insert for the Hermle Z327-200-BUK swing-out bucket on the Z327 / Z327-K chassis 4-place rotor — sold as a 2-pack. Each insert holds 1 × 50 mL round-bottom tube; the 4-bucket rotor configuration takes 2 packs (4 inserts) for full symmetric loading, delivering 4 × 50 mL round-bottom tubes per cycle (200 mL total) in swing-out geometry. Sister insert to the A50 (50 mL Falcon conical) and A50SS (50 mL self-standing skirted) — both serve the same 50 mL workflow envelope but for different tube geometries.
Key Specifications
| SKU | Z327-200-A50R |
| Type | Tube insert — 1 × 50 mL round-bottom |
| Pack quantity | 2 inserts per SKU (full 4-bucket configuration takes 2 units = 4 inserts) |
| Per-insert capacity | 1 × 50 mL round-bottom tube |
| Per-cycle throughput (full configuration) | 4 × 50 mL round-bottom = 200 mL total per cycle |
| Tube format | 50 mL round-bottom glass or polypropylene (29 mm OD, 95–103 mm length) |
| Compatible bucket | Z327-200-BUK (with Z327-200-LID for hermetic seal) |
| Compatible rotor | Z327-200 (or Z327-200-AC rotor body without buckets) |
| Compatible chassis | Z327, Z327-K (refrigerated), and -E voltage variants |
| Max. operating speed (with insert + tube) | Per Z327-200 spec — 5,000 rpm at 4,136 × g maximum |
| Max. radius | 14.8 cm (0.2 cm radius correction with this insert) |
| Geometry | Swing-out (0–90°) — flat sediment surface |
| List price | $288 (2-pack) |
Compatibility & When to Order This SKU
Insert pairing — Z327-200-BUK swing-out bucket only. Order this SKU when:
- Adding 50 mL round-bottom tube workflow to an existing Z327 / Z327-K + Z327-200 install — order 2 units for full 4-bucket symmetric loading.
- Glass round-bottom 50 mL workflows when the protocol requires glass for organic-solvent compatibility, repeated autoclave reuse, or precise volume measurement.
- Cold-chain glass round-bottom 50 mL workflows on the Z327-K refrigerated chassis.
- Optional BSL-2 + workflow with the Z327-200-LID engaged for hermetic containment.
- Replacement inserts if a current set is damaged or worn.
Application Profile
Round-bottom vs conical (vs the A50 sister). The A50R holds 50 mL round-bottom tubes (95–103 mm length). The A50 sister insert holds 50 mL Falcon-style conical (116 mm length). The A50SS holds 50 mL self-standing skirted-base conical (116 mm length). The three formats have different shoulder geometries; using the wrong insert risks tube fit problems and balance issues.
Glass tube workflow compatibility. 50 mL round-bottom glass tubes support workflows requiring glass for organic-solvent compatibility, repeated autoclave reuse, or precise volume measurement (graduated glass tubes).
4-bucket symmetric loading. All 4 buckets must use matching A50R inserts for symmetric balance.
Optional hermetic seal via LID. Compatible with the Z327-200-LID for BSL-2 + 50 mL round-bottom workflows.
Design Architecture
Cold-chain glass-compatible 50 mL workflows — organic-extraction, chemical-prep, and precipitation workflows in 50 mL round-bottom glass at refrigerated temperature.
BSL-2 + 50 mL round-bottom glass clinical-research prep — clinical-research workflows handling potentially infectious patient samples in 50 mL round-bottom glass that require both cold-chain and biosafety containment.
Cell-culture prep in 50 mL round-bottom glass — when cell-culture protocols specify round-bottom glass for resuspension volume measurement or solvent-compatibility requirements.
Cold-chain protein prep in glass round-bottom — TCA, ammonium-sulfate, organic-precipitation in 50 mL round-bottom glass on the refrigerated swing-out chassis.
Educational and graduate-level chemistry / biochemistry teaching — workflows specifying round-bottom glass at cold-chain temperature for protocol-fidelity teaching.
Cross-format Z327-K core facilities — labs that run both round-bottom and conical 50 mL workflows benefit from stocking both A50 and A50R inserts on the same bucket.
Ordering & Configuration
4-bucket symmetric loading. All 4 buckets must use matching A50R inserts. Mixed-bucket configurations produce mass asymmetry.
Optional hermetic-seal engagement. When using the Z327-200-LID for biosafety workflows, confirm the lid is fully engaged on each bucket before each spin.
Tube cap confirmation. All round-bottom tubes must be capped securely before spinning at 5,000 rpm.
Z327-200-A50R ships as 2 inserts. 50 mL round-bottom tubes (glass or polypropylene) are operator-supplied. The Z327-200-BUK bucket is sold separately.
Confirm at quote: bucket pairing (Z327-200-BUK in inventory; Z327-200-LID if biosafety required), chassis pairing (Z327 or Z327-K), tube format (50 mL round-bottom; conical uses A50, self-standing uses A50SS), pack count (2 packs for full 4-bucket configuration), and lead time.
Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts.
Z327-200 50 mL insert variants (Z327 / Z327-K chassis):
- Z327-200-A50R (this product) — 50 mL round-bottom (95–103 mm)
- Z327-200-A50 — 50 mL Falcon-style conical (116 mm)
- Z327-200-A50SS — 50 mL self-standing skirted-base conical (116 mm)
Match the insert to the 50 mL tube envelope.
