50 mL Conical Insert for Z326-100H Sealed Bucket (2/pk)

The Z326-100H-A50 is the 50 mL Falcon conical insert for the Hermle Z326-100H sealed bucket on the Z327 / Z327-K chassis 4-place swing-out rotor, sold as a 2-pack. Each insert holds 1 × 50 mL conical tube; the 4-bucket rotor configuration takes 2 packs (4 inserts) for full symmetric loading, delivering 4 × 50 mL conical tubes per cycle in hermetically sealed swing-out geometry on a refrigerated chassis. The 50 mL conical workhorse insert for the Z326-100H family, paired with the A50R (round-bottom variant) for tube-geometry-specific 50 mL workflows.

Key Specifications

Compatibility & When to Order This SKU

Insert pairing: Z326-100H sealed bucket only. Order this SKU when:

1. Adding 50 mL conical workflow to an existing Z327 / Z327-K + Z326-100H install. Order 2 units for full 4-bucket symmetric loading (200 mL total per cycle).
2. Cold-chain 50 mL clinical-research and bioprocess prep: refrigerated cell-culture pellet recovery, density-gradient prep, biobank aliquot prep at cold-chain integrity.
3. BSL-2 + 50 mL workflows: when 50 mL prep handles potentially infectious patient samples or genetically modified organisms requiring aerosol containment.
4. Replacement inserts if a current set is damaged or worn.

Application Profile

Hermetically sealed cold-chain 50 mL workflow. The Z326-100H bucket + Z326-100H-LID + A50 insert + Z327-K chassis combination delivers 50 mL conical workflows with cold-chain (2 °C at max speed), biosafety containment (sealed bucket envelope), and swing-out flat-sediment geometry simultaneously.

Conical vs round-bottom. The A50 insert holds conical 50 mL tubes (Falcon, Corning, equivalent). For 50 mL round-bottom workflows (RB Glass, Nalgene round-bottom), use the A50R insert.

4-bucket symmetric loading. All 4 buckets must use matching A50 inserts for symmetric balance.

Lower per-cycle throughput than non-sealed alternatives. The Z326-100H-A50 is 4 tubes/cycle (1 per bucket); the non-sealed Z306-100-A50 on the Z307 chassis is 28 tubes/cycle (7 per bucket). The Z326-100H trades throughput for biosafety containment and refrigeration integrity. Pick this SKU when biosafety / cold-chain matters more than per-cycle throughput.

Design Architecture

Cold-chain BSL-2 cell-culture pellet recovery in 50 mL: clinical-research and bioprocess workflows that handle potentially infectious cell-culture samples or genetically modified organisms in 50 mL conical batches.

Cold-chain 50 mL biobank patient-sample fractionation: biobank intake workflows fractionating patient samples in 50 mL conical for downstream cryostorage with cold-chain integrity.

Density-gradient prep at cold-chain. Ficoll, Percoll, sucrose-gradient prep in 50 mL conical where the swing-out flat-sediment geometry preserves gradient interfaces and refrigeration preserves gradient stability.

Viral and bacterial culture prep at BSL-2: 50 mL viral or bacterial culture pellet recovery requiring aerosol containment during the spin step.

Bench-scale cold-chain protein prep. Protein precipitation, organic extraction in 50 mL working volume on the refrigerated biosafety chassis.

Cross-format Z327-K chassis flexibility: labs running 50 mL conical alongside other tube and bottle formats on the cold-chain biosafety chassis benefit from stocking the full Z326-100H insert family.

Ordering & Configuration

4-bucket symmetric loading. All 4 buckets must use matching A50 inserts. Mixed-bucket configurations produce mass asymmetry.

Hermetic-seal engagement. Per Hermle service instructions, the Z326-100H-LID must be fully engaged on each bucket before each spin to maintain the hermetic envelope at 4,080 × g.

Tube cap confirmation. All conical tubes must be securely capped before spinning at 5,000 rpm.

Z326-100H-A50 ships as 2 inserts. 50 mL conical tubes are operator-supplied (commodity Falcon / Corning / equivalent formats). The Z326-100H bucket and Z326-100H-LID are sold separately.

Confirm at quote: bucket pairing (Z326-100H + Z326-100H-LID in inventory), chassis pairing (Z327 or Z327-K), tube format (50 mL conical Falcon-style), workflow drivers (cold-chain, biosafety), pack count (2 packs for full 4-bucket configuration), and lead time.

Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts. Hermetic-seal interface is a planned-inspection item.

Z326-100H 50 mL insert pair (Z327 / Z327-K chassis):

- Z326-100H-A50 (this product): 50 mL Falcon conical, sealable

- Z326-100H-A50R: 50 mL round-bottom (different tube geometry)

Match the insert to the workflow's tube geometry. Both deliver 4-tube parallel throughput on the full 4-bucket configuration with cold-chain and biosafety containment when the bucket lid is engaged.