The Z327-200-A50SS is the 50 mL self-standing conical tube insert for the Hermle Z327-200-BUK swing-out bucket on the Z327 / Z327-K chassis 4-place rotor — sold as a 2-pack. Each insert holds 1 × 50 mL skirted-base self-standing conical tube; the 4-bucket rotor configuration takes 2 packs (4 inserts) for full symmetric loading, delivering 4 × 50 mL self-standing tubes per cycle in swing-out geometry. The SS suffix denotes self-standing — sized specifically for 50 mL conical tubes with an integrated skirted base that lets the tube stand upright on the bench without a rack (Corning CentriStar, BD Falcon Self-Standing, and similar self-standing 50 mL formats).
Key Specifications
| SKU | Z327-200-A50SS |
| Type | Tube insert — 1 × 50 mL self-standing skirted-base conical |
| Pack quantity | 2 inserts per SKU (full 4-bucket configuration takes 2 units = 4 inserts) |
| Per-insert capacity | 1 × 50 mL self-standing skirted-base conical tube |
| Per-cycle throughput (full configuration) | 4 × 50 mL self-standing per cycle |
| Tube format | 50 mL skirted-base self-standing conical (29 mm OD, 116 mm length) |
| Compatible bucket | Z327-200-BUK (with Z327-200-LID for hermetic seal) |
| Compatible rotor | Z327-200 (or Z327-200-AC rotor body without buckets) |
| Compatible chassis | Z327, Z327-K (refrigerated), and -E voltage variants |
| Max. operating speed (with insert + tube) | Per Z327-200 spec — 5,000 rpm at 4,136 × g maximum |
| Max. radius | 14.8 cm (0.2 cm radius correction with this insert) |
| Geometry | Swing-out (0–90°) — flat sediment surface |
| List price | $288 (2-pack) |
Compatibility & When to Order This SKU
Insert pairing — Z327-200-BUK swing-out bucket only. Order this SKU when:
- Adding self-standing 50 mL conical workflow to an existing Z327 / Z327-K + Z327-200 install — order 2 units for full 4-bucket symmetric loading.
- Workflows standardized on Corning CentriStar, BD Falcon Self-Standing, or similar skirted-base 50 mL conical formats — these formats have integrated bench-standing geometry that the standard A50 insert (sized for non-skirted Falcon-style) doesn't accommodate.
- Multi-step molecular-biology or clinical-research protocols where the self-standing tube's bench ergonomics matter across multiple workflow steps.
- Optional cold-chain workflow on the Z327-K refrigerated chassis.
- Optional BSL-2 + workflow with the Z327-200-LID engaged for hermetic containment.
- Replacement inserts if a current set is damaged or worn.
Why a Self-Standing Variant Matters
Self-standing 50 mL conical tubes are popular in clinical-research workflows because they sit upright on the bench without a rack — a workflow ergonomic improvement during multi-step protocols (cap on, add reagent, cap off, vortex, place back upright, etc.). The skirted base extends the tube's overall length by ~10–15 mm beyond the standard non-skirted 50 mL conical, and the skirt also widens the tube's effective bench footprint.
The standard A50 insert is sized for non-skirted tubes; loading a skirted-base tube into the A50 insert risks fit problems — the skirt may not seat correctly, leaving the tube tilted within the insert envelope, producing imbalance at the rotor's RCF. The A50SS insert is dimensioned for the longer skirted format — confirm tube format matches the insert at order.
Application Profile
Workflows standardized on self-standing 50 mL conical formats — clinical-research labs and biobanks that have standardized on self-standing tubes for workflow ergonomics get matching insert support without changing tube format.
Cold-chain self-standing 50 mL prep on Z327-K — refrigerated patient sample fractionation, biobank aliquot prep, density-gradient prep in self-standing format.
Multi-step molecular-biology workflows — DNA / RNA extraction, protein-prep, and similar multi-step protocols where the self-standing tube's bench ergonomics matter, with centrifugation steps in between.
BSL-2 + self-standing workflows — when BSL-2 + clinical-research workflows handle samples in self-standing 50 mL conical format and require hermetic containment via the Z327-200-LID.
Mixed self-standing / standard 50 mL conical workflow consolidation — when the lab uses both formats and the workflow benefits from both insert variants on the same chassis.
Cross-format Z327-K core facilities — shared cores supporting researchers who use different 50 mL conical brands benefit from stocking both A50 and A50SS inserts.
Ordering & Configuration
4-bucket symmetric loading. All 4 buckets must use matching A50SS inserts for symmetric balance.
Insert seat verification. Confirm each insert is fully seated in its bucket envelope before loading the self-standing 50 mL tube — the longer tube length means the insert depth and tube clearance both matter.
Optional hermetic-seal engagement. When using the Z327-200-LID for biosafety workflows, confirm the lid is fully engaged on each bucket before each spin.
Tube cap confirmation. All 50 mL conical tubes must be securely capped before spinning at 5,000 rpm.
Z327-200-A50SS ships as 2 inserts. 50 mL self-standing skirted-base conical tubes are operator-supplied (commodity formats from Corning, BD Falcon, Greiner, and similar suppliers). The Z327-200-BUK bucket and (optional) Z327-200-LID are sold separately.
Confirm at quote: bucket pairing (Z327-200-BUK in inventory; Z327-200-LID if biosafety required), chassis pairing (Z327 or Z327-K), tube format (50 mL self-standing skirted-base, 116 mm length; standard non-skirted Falcon-style uses A50 instead), pack count (2 packs for full 4-bucket configuration), and lead time.
Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts.
Z327-200 50 mL insert variants (Z327 / Z327-K chassis):
- Z327-200-A50SS (this product) — 50 mL self-standing skirted-base conical (116 mm)
- Z327-200-A50 — 50 mL Falcon-style conical (116 mm, non-skirted)
- Z327-200-A50R — 50 mL round-bottom (95–103 mm)
Match the insert to the 50 mL tube format. The A50SS is the right pick for self-standing skirted-base conical formats.
