50 mL Conical Insert for Z446-04500 (2/pk)

The Z446-500-A50 is the 50 mL Falcon conical tube insert for the Hermle Z446-04500 4 × 500 mL angle rotor on the Z446 / Z446-K chassis — sold as a 2-pack. Each insert holds 1 × 50 mL conical tube; the 4-position rotor takes 2 packs (4 inserts) for full symmetric loading, delivering 4 × 50 mL conical tubes per cycle (200 mL total) at the host rotor's high-RCF range (10,367 × g). The single-tube-per-insert configuration delivers maximum-RCF processing for the 50 mL conical workflow on the bulk-volume Z446 platform.

Key Specifications

Compatibility & When to Order This SKU

Insert pairing — Z446-04500 500 mL angle rotor only. Order this SKU when:

1. Adding 50 mL Falcon conical workflow to a Z446-04500 install — order 2 units for full 4-position symmetric loading (4 tubes/cycle).
2. High-RCF cold-chain 50 mL conical prep — when the workflow needs the >10,000 × g range for 50 mL conical tubes at refrigerated temperature.
3. Mixed 500 / 50 mL workflow consolidation — when the chassis primarily runs 500 mL bulk-volume workflows but occasional 50 mL conical processing arises.
4. Replacement inserts if a current set is damaged or worn.

Why High-RCF 50 mL Conical Matters

The Z446-500-A50 + Z446-04500 + Z446-K refrigerated chassis combination delivers a configuration not common in the broader centrifuge market: 50 mL conical workflow at >10,000 × g with 4 °C cold-chain integrity. Most dedicated 50 mL angle rotors top out at 4,000–8,000 × g; the 500 mL host rotor's higher max speed pushes 50 mL workflow into the high-RCF range typically reserved for smaller-volume tubes.

This matters when:

• Cell-pellet recovery in 50 mL conical needs tight pellet compaction at high RCF
• Density-gradient prep in 50 mL conical needs >10,000 × g for clean phase separation
• Cold-chain preserves protein integrity, gradient stability, or temperature-sensitive analytes through a high-force spin step

The 4-tube-per-cycle throughput is lower than dedicated 50 mL rotors (Z326-1050 at 10 × 50 mL, Z206-0650 at 6 × 50 mL), but the trade-off is justified when the workflow specifically needs the high-RCF range. For higher-throughput 50 mL on the Z446 chassis, the Z446-750-A50R (28 × 50 mL round-bottom/cycle) and Z446-750-B50 (28 × 50 mL conical/cycle) inserts on the Z446-750-AC swing-out rotor are alternatives.

Application Profile

High-RCF cold-chain 50 mL cell-pellet recovery — pelleting cells from 50 mL suspension culture batches at high RCF and refrigerated temperature for downstream-stable preparation.

High-RCF 50 mL conical density-gradient prep — Ficoll, Percoll, sucrose-gradient prep at 4 × 50 mL parallel preps at >10,000 × g.

Cold-chain protein-prep workflows in 50 mL conical — TCA, ammonium-sulfate, organic-precipitation in 50 mL working volume at high RCF on the refrigerated chassis.

Clinical-research and biobank prep at 50 mL high-RCF cold-chain — patient sample fractionation, aliquot prep, freeze-storage workflows where the >10,000 × g range matters.

Mixed 500 / 50 mL workflow consolidation — when the lab's primary chassis is the Z446-04500 for 500 mL bulk workflows and 50 mL conical workflows are part of the protocol.

Cross-format Z446-K core facilities — shared cores running mixed bottle and tube workflows on the cold-chain high-RCF chassis.

Ordering & Configuration

4-position symmetric loading. All 4 positions on the host rotor must use matching A50 inserts for symmetric balance at 8,000 rpm.

Insert seat verification. Confirm each insert is fully seated in its 500 mL position before loading the 50 mL conical tube.

Tube cap confirmation. All 50 mL conical tubes must be securely capped before spinning at 8,000 rpm — at the host rotor's high RCF, cap integrity is critical.

Z446-500-A50 ships as 2 inserts. 50 mL conical tubes are operator-supplied (commodity Falcon / Corning / equivalent formats).

Confirm at quote: host rotor (Z446-04500 in inventory), chassis pairing (Z446 or Z446-K), tube format (50 mL conical Falcon-style; round-bottom uses A50R), pack count (2 packs for full 4-position configuration), and lead time.

Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts.

Z446-500 50 mL insert pair (Z446-04500 4 × 500 mL angle rotor):

- Z446-500-A50 (this product) — 1 × 50 mL Falcon conical (4/cycle)

- Z446-500-A50R — 3 × 50 mL round-bottom (12/cycle, higher throughput, round tube envelope)

Match the insert to the workflow's tube format and target throughput. The A50 is the right pick for single-tube-per-position 50 mL conical at the host rotor's full RCF range.