50 mL Conical Insert for Z327-200-BUK (2/pk)

The Z327-200-A50 is the 50 mL Falcon conical tube insert for the Hermle Z327-200-BUK swing-out bucket on the Z327 / Z327-K chassis 4-place rotor — sold as a 2-pack. Each insert holds 1 × 50 mL conical tube; the 4-bucket rotor configuration takes 2 packs (4 inserts) for full symmetric loading, delivering 4 × 50 mL conical tubes per cycle (200 mL total) in swing-out geometry. Sister insert to the A50R (50 mL round-bottom) and A50SS (50 mL self-standing skirted) — three 50 mL variants for different tube envelopes.

Key Specifications

Compatibility & When to Order This SKU

Insert pairing — Z327-200-BUK swing-out bucket only. Order this SKU when:

1. Adding 50 mL Falcon conical workflow to an existing Z327 / Z327-K + Z327-200 install — order 2 units for full 4-bucket symmetric loading (200 mL total per cycle).
2. Cold-chain swing-out 50 mL conical prep — refrigerated cell-culture pellet recovery, density-gradient prep, biobank aliquot prep at 4 tubes per cycle.
3. Optional BSL-2 + 50 mL workflows — combine with the Z327-200-LID for hermetic containment when handling potentially infectious patient samples.
4. Replacement inserts if a current set is damaged or worn.

Conical, Round, Self-Standing — Three 50 mL Variants

The Z327-200 insert family includes three distinct 50 mL variants for different tube envelopes:

Match the insert to the workflow's tube envelope. Using the wrong-envelope insert risks tube fit problems and balance issues at the rotor's RCF range.

Application Profile

Swing-out 50 mL conical workflow. The Z327-200 + A50 combination delivers swing-out 50 mL conical processing — flat sediment, clean phase boundaries for serum/plasma–cell-pellet separation, easier downstream supernatant aspiration than fixed-angle rotors deliver.

4-bucket symmetric loading. All 4 buckets must use matching A50 inserts for symmetric balance.

Optional hermetic seal via LID. When combined with the Z327-200-LID, the bucket creates a hermetic envelope for BSL-2 + 50 mL workflows.

Design Architecture

Cold-chain 50 mL clinical-research and biobank prep — patient sample fractionation, aliquot prep, freeze-storage workflows at 4 × 50 mL conical per cycle on the cold-chain swing-out chassis.

Cell-culture pellet recovery in 50 mL — pelleting cells from suspension culture batches with flat sediment surface for clean resuspension.

Density-gradient prep in 50 mL conical — Ficoll, Percoll, sucrose-gradient prep where the swing-out flat-sediment geometry preserves gradient interfaces.

Bench-scale cold-chain protein prep — TCA, ammonium-sulfate, organic-precipitation in 50 mL working volume across 4 parallel preps with refrigeration.

BSL-2 + clinical-blood-component prep at 50 mL — clinical-research workflows handling potentially infectious patient samples in 50 mL conical that require both cold-chain and biosafety containment.

Cross-format Z327-K core facilities — labs running 50 mL conical alongside other tube and bottle formats benefit from stocking the Z327-200 insert family.

Ordering & Configuration

4-bucket symmetric loading. All 4 buckets must use matching A50 inserts. Mixed-bucket configurations produce mass asymmetry.

Optional hermetic-seal engagement. When using the Z327-200-LID for biosafety workflows, confirm the lid is fully engaged on each bucket before each spin.

Tube cap confirmation. All conical tubes must be securely capped before spinning at 5,000 rpm.

Z327-200-A50 ships as 2 inserts. 50 mL conical tubes are operator-supplied (commodity Falcon / Corning / equivalent formats; for self-standing skirted-base 50 mL conical, use the A50SS insert instead). The Z327-200-BUK bucket is sold separately.

Confirm at quote: bucket pairing (Z327-200-BUK in inventory; Z327-200-LID if biosafety required), chassis pairing (Z327 or Z327-K), tube format (50 mL Falcon conical; round-bottom uses A50R; self-standing uses A50SS), pack count (2 packs for full 4-bucket configuration), and lead time.

Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts.

Z327-200 50 mL insert variants (Z327 / Z327-K chassis):

- Z327-200-A50 (this product) — 50 mL Falcon-style conical (116 mm)

- Z327-200-A50R — 50 mL round-bottom (95–103 mm)

- Z327-200-A50SS — 50 mL self-standing skirted-base conical (116 mm)

Match the insert to the 50 mL tube envelope.