The Z327-200-A30 is the 30 mL conical / round-bottom tube insert for the Hermle Z327-200-BUK swing-out bucket on the Z327 / Z327-K chassis 4-place rotor — sold as a 2-pack. Each insert holds 1 × 30 mL tube; the 4-bucket rotor configuration takes 2 packs (4 inserts) for full symmetric loading, delivering 4 × 30 mL tubes per cycle in swing-out geometry. The 30 mL format sits in the intermediate volume range — between 15 mL conical and 50 mL conical workflows — useful for bioprocess intermediate prep and clinical-research workflows specifying 30 mL working volume.
Key Specifications
| SKU | Z327-200-A30 |
| Type | Tube insert — 1 × 30 mL conical or round |
| Pack quantity | 2 inserts per SKU (full 4-bucket configuration takes 2 units = 4 inserts) |
| Per-insert capacity | 1 × 30 mL tube |
| Per-cycle throughput (full configuration) | 4 × 30 mL per cycle |
| Tube format | 30 mL conical or round-bottom (25 mm OD, 90–110 mm length) |
| Compatible bucket | Z327-200-BUK (with Z327-200-LID for hermetic seal) |
| Compatible rotor | Z327-200 (or Z327-200-AC rotor body without buckets) |
| Compatible chassis | Z327, Z327-K (refrigerated), and -E voltage variants |
| Max. operating speed (with insert + tube) | Per Z327-200 spec — 5,000 rpm at 4,136 × g maximum |
| Max. radius | 14.8 cm (0.2 cm radius correction with this insert) |
| Geometry | Swing-out (0–90°) — flat sediment surface |
| List price | $288 (2-pack) |
Compatibility & When to Order This SKU
Insert pairing — Z327-200-BUK swing-out bucket only. Order this SKU when:
- Adding 30 mL tube workflow to an existing Z327 / Z327-K + Z327-200 install — order 2 units for full 4-bucket symmetric loading (4 tubes/cycle).
- Bioprocess intermediate prep at 30 mL — vaccine-development and bioprocess workflows that batch 30 mL volumes for intermediate concentration steps.
- Optional cold-chain workflow on the Z327-K refrigerated chassis.
- Optional BSL-2 + workflow with the Z327-200-LID engaged for hermetic containment.
- Replacement inserts if a current set is damaged or worn.
A25 vs A30 — Format Distinction
The Z327-200 intermediate-volume insert pair offers similar envelope dimensions for two distinct tube formats:
| Insert | Tube format | Length envelope | Common use |
| Z327-200-A25 | 25 mL Universal | 95–105 mm | Universal-format pooled samples, bioassay prep |
| Z327-200-A30 (this SKU) | 30 mL conical or round | 90–110 mm | Bioprocess intermediates, mid-volume cell-prep |
Both inserts share the 25 mm OD envelope; the choice depends on which tube format the workflow specifies. The 30 mL conical / round format is more common in bioprocess and clinical-research mid-volume workflows; the 25 mL Universal is more common in pooled-sample and bioassay workflows.
Application Profile
Bioprocess intermediate prep at 30 mL — vaccine-development, recombinant-protein, and cell-culture workflows that batch 30 mL bottles for intermediate concentration steps between smaller and larger volumes.
Mid-volume cell harvest workflows — pelleting cells from 30 mL suspension batches at 4 tubes per cycle.
Cold-chain mid-volume aliquot prep on Z327-K — refrigerated 30 mL aliquot workflows for cold-stable bioprocess intermediates.
Mid-volume protein-prep workflows — TCA, ammonium-sulfate, organic-precipitation in 30 mL working volume on the swing-out platform.
BSL-2 + 30 mL workflows — recombinant-protein expression and bioprocess prep handling genetically modified organisms in 30 mL bottle format with hermetic containment.
Clinical-research mid-volume aliquot prep — pooled patient sample workflows in 30 mL working volume on the cold-chain swing-out chassis.
Cross-format Z327-K core facilities — labs running 30 mL alongside 15 mL conical and 50 mL conical workflows on the same Z327-200 + Z327-200-BUK inventory.
Ordering & Configuration
4-bucket symmetric loading. All 4 buckets must use matching A30 inserts for symmetric balance.
Insert seat verification. Confirm each insert is fully seated in its bucket before loading the 30 mL tube.
Tube cap confirmation. All 30 mL tubes must be securely capped before spinning at 5,000 rpm.
Z327-200-A30 ships as 2 inserts. 30 mL conical or round-bottom tubes are operator-supplied.
Confirm at quote: bucket pairing (Z327-200-BUK in inventory; Z327-200-LID if biosafety required), chassis pairing (Z327 or Z327-K), tube format (30 mL conical or round, 25 mm OD), pack count (2 packs for full 4-bucket configuration), and lead time.
Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts.
Z327-200 intermediate-volume insert family (Z327 / Z327-K chassis):
- Z327-200-A30 (this product) — 30 mL conical or round (90–110 mm length)
- Z327-200-A25 — 25 mL Universal (95–105 mm length, similar envelope)
Match the insert to the workflow's intermediate-volume tube format. Both deliver 4-tube parallel throughput on the full 4-bucket configuration.
