3 × 50 mL Conical Insert for Z366-250 Bucket (2/pk)

The Z366-250-A50 is a 3-position 50 mL conical tube insert for the Hermle Z366-250 swing-out bucket on the Z366 / Z366-K chassis 4-place rotor, sold as a 2-pack. Each insert holds 3 × 50 mL conical tubes; the 4-bucket rotor configuration takes 2 packs (4 inserts) for full symmetric loading, delivering 12 × 50 mL conical tubes per cycle in swing-out geometry on the deep cold-chain Z366-K chassis (−2 °C at max speed). Sister insert to the Z366-250-A50R (50 mL round-bottom), same volume, different tube envelope geometry.

Key Specifications

Compatibility & When to Order This SKU

Insert pairing: Z366-250 swing-out bucket only. Order this SKU when:

1. Adding 50 mL conical workflow to an existing Z366 / Z366-K + Z366-250 install. Order 2 units for full 4-bucket symmetric loading (12 tubes/cycle).
2. Deep cold-chain 50 mL conical prep: when the workflow needs the Z366-K's −2 °C cold-chain integrity for cold-stable analyte preservation in 50 mL conical format.
3. Bucket-insert flexibility. When the lab uses the same bucket for multiple tube/bottle formats and needs the bucket-insert path (vs the direct-fit Z366-250-B50 alternative on the HC rotor variant).
4. Replacement inserts if a current set is damaged or worn.

A50 vs B50: Bucket-Insert vs Direct-Fit Trade-Off

The Z366 chassis offers two paths for swing-out 50 mL conical workflows:

Pick the A50 when the lab uses the bucket for multiple formats (the bucket holds A10, A100, A20, A250, etc.); pick the B50 when 50 mL conical is the dominant workflow and throughput matters most.

Application Profile

4-bucket symmetric loading. All 4 buckets must use matching A50 inserts for symmetric balance.

Per-insert symmetric loading. Within each insert, the 3 conical tubes should be balanced symmetrically. Pair tubes by mass with the third position symmetric to the insert's central axis.

Design Architecture

Deep cold-chain 50 mL conical clinical-research and biobank prep: patient sample fractionation, aliquot prep, freeze-storage workflows at 12 × 50 mL conical per cycle on the near-freezing cold-chain swing-out chassis.

Cell-culture pellet recovery in 50 mL: pelleting cells from suspension culture batches at deep cold-chain temperature for downstream-stable cell preparation.

Density-gradient prep in 50 mL: Ficoll, Percoll, sucrose-gradient prep where the swing-out flat-sediment geometry preserves gradient interfaces and the deep cold-chain integrity supports gradient stability.

Cold-stable protein prep at 50 mL: TCA, ammonium-sulfate, organic-precipitation in 50 mL working volume at near-freezing temperature.

Mixed-format Z366-K core facilities: labs that handle multiple tube/bottle formats on the same chassis benefit from the bucket-insert approach over dedicated direct-fit buckets.

Educational and graduate-level teaching. Molecular biology and clinical-research curricula using 50 mL conical at moderate throughput on the deep cold-chain chassis.

Ordering & Configuration

4-bucket symmetric loading. All 4 buckets must use matching A50 inserts. Mixed-bucket configurations produce mass asymmetry.

Tube cap confirmation. All 50 mL conical tubes must be securely capped before spinning at 4,500 rpm.

Z366-250-A50 ships as 2 inserts. 50 mL conical tubes are operator-supplied (commodity Falcon / Corning / equivalent formats). The Z366-250 bucket is sold separately.

Confirm at quote: bucket pairing (Z366-250 in inventory), chassis pairing (Z366 or Z366-K), tube format (50 mL conical Falcon-style; round-bottom uses A50R), workflow throughput need (A50 at 12/cycle vs B50 at 16/cycle), pack count (2 packs for full 4-bucket configuration), and lead time.

Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts.

Z366-250 50 mL workflow paths (Z366 / Z366-K chassis):

- Z366-250-A50 (this product): 3 × 50 mL conical insert (12/cycle, bucket-insert flexibility)

- Z366-250-A50R: 3 × 50 mL round-bottom insert (different tube envelope)

- Z366-250-B50: 4 × 50 mL conical direct-fit bucket (16/cycle, dedicated higher throughput on HC rotor variant)

Pick A50 for bucket-insert flexibility; pick B50 when 50 mL conical throughput is the dominant workflow driver.