25 mL Universal Tube Insert for Z327-200-BUK (2/pk)

The Z327-200-A25 is the 25 mL Universal tube insert for the Hermle Z327-200-BUK swing-out bucket on the Z327 / Z327-K chassis 4-place rotor — sold as a 2-pack. Each insert holds 1 × 25 mL Universal tube; the 4-bucket rotor configuration takes 2 packs (4 inserts) for full symmetric loading, delivering 4 × 25 mL Universal tubes per cycle in swing-out geometry. The 25 mL format sits between 15 mL and 50 mL conical workflows — useful for clinical-research and bioprocess workflows specifying 25 mL working volume (some pooled-sample protocols, some bioassay sample-prep workflows).

Key Specifications

Compatibility & When to Order This SKU

Insert pairing — Z327-200-BUK swing-out bucket only. Order this SKU when:

1. Adding 25 mL Universal tube workflow to an existing Z327 / Z327-K + Z327-200 install — order 2 units for full 4-bucket symmetric loading (4 tubes/cycle).
2. Mid-volume aliquot prep workflows that batch 25 mL pooled samples or intermediate aliquots.
3. Optional cold-chain workflow on the Z327-K refrigerated chassis.
4. Optional BSL-2 + workflow with the Z327-200-LID engaged for hermetic containment.
5. Replacement inserts if a current set is damaged or worn.

Why 25 mL Matters as an Intermediate Volume

The 25 mL tube format sits in an intermediate volume range between common formats:

• 15 mL conical — standard clinical-research workflow tube
• 25 mL Universal (this insert) — pooled-sample workflows, intermediate aliquot prep, some bioassay protocols
• 50 mL conical — institutional clinical-research and bioprocess workflows

Some workflows specifically require 25 mL working volume — pooled patient sample workflows where 4 × 6.25 mL aliquots combine into a 25 mL pool, certain bioassay sample-prep protocols, certain chemical-prep intermediate volumes — where 15 mL is too small and 50 mL is more sample than the protocol specifies. The Z327-200-A25 insert delivers this intermediate volume on the Z327-200 swing-out platform.

Application Profile

Pooled patient sample prep at 25 mL — clinical-research workflows that pool patient samples into 25 mL working volume for combined-batch processing.

Bioassay sample-prep at 25 mL — bioassay protocols specifying 25 mL working volume for the sample-prep step.

Cold-chain mid-volume aliquot prep on Z327-K — refrigerated 25 mL aliquot workflows for cold-stable analyte prep.

Mid-volume cell-culture pellet recovery in 25 mL Universal — when cell-culture batches are sized to the 25 mL Universal format.

BSL-2 + 25 mL aliquot workflows — when potentially infectious pooled samples in 25 mL Universal format require aerosol containment via the Z327-200-LID.

Cross-format Z327-K core facilities — labs running 25 mL Universal alongside other tube and bottle formats on the same Z327-200 + Z327-200-BUK inventory.

Educational and graduate-level teaching — workflows specifying 25 mL Universal format for protocol-fidelity teaching exercises.

Ordering & Configuration

4-bucket symmetric loading. All 4 buckets must use matching A25 inserts for symmetric balance.

Insert seat verification. Confirm each insert is fully seated in its bucket before loading the 25 mL tube.

Tube cap confirmation. All 25 mL tubes must be securely capped before spinning at 5,000 rpm.

Z327-200-A25 ships as 2 inserts. 25 mL Universal tubes are operator-supplied (commodity Universal-format tubes from major commercial suppliers).

Confirm at quote: bucket pairing (Z327-200-BUK in inventory; Z327-200-LID if biosafety required), chassis pairing (Z327 or Z327-K), tube format (25 mL Universal, 25 mM OD), pack count (2 packs for full 4-bucket configuration), and lead time.

Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts.

Z327-200 intermediate-volume insert family (Z327 / Z327-K chassis):

- Z327-200-A25 (this product) — 25 mL Universal (95–105 mm length)

- Z327-200-A30 — 30 mL conical (90–110 mm length, similar envelope, conical tube)

Match the insert to the workflow's intermediate-volume tube format — Universal (25 mL) vs conical (30 mL).