250 mL Flat Bottle Insert for Z366-250 Bucket (2/pk)

The Z366-250-A250 is the 250 mL flat-bottle insert for the Hermle Z366-250 swing-out bucket on the Z366 / Z366-K chassis 4-place rotor, sold as a 2-pack. Each insert holds 1 × 250 mL bottle; the 4-bucket rotor configuration takes 2 packs (4 inserts) for full symmetric loading, delivering 4 × 250 mL bottles per cycle (1 L total per cycle) in swing-out geometry on the deep cold-chain Z366-K chassis. The largest bottle insert in the Z366-250 swing-out family, sized for institutional bulk-prep workflows requiring near-freezing cold-chain integrity (−2 °C at max speed).

Key Specifications

Compatibility & When to Order This SKU

Insert pairing: Z366-250 swing-out bucket only. Order this SKU when:

1. Adding 250 mL bulk-volume bottle workflow to an existing Z366 / Z366-K + Z366-250 install. Order 2 units for full 4-bucket symmetric loading (1 L total per cycle).
2. Deep cold-chain bulk-volume bottle workflows. When the workflow needs the Z366-K's −2 °C cold-chain integrity for cold-stable bulk preparation.
3. Institutional bulk cell-prep workflows that batch 4 × 250 mL cultures or aliquots per cycle.
4. Replacement inserts if a current set is damaged or worn.

Application Profile

4-bucket symmetric loading. All 4 buckets must use matching A250 inserts for symmetric balance. With full configuration, each cycle processes 1 L of working volume, the largest single-cycle volume in the Z366-250 swing-out insert family.

Bottle format precision. The A250 envelope is dimensioned for the 62 mm OD × 110–130 mm length range. Bottles outside this dimensional range may not seat properly; verify dimensions if using non-standard bottle suppliers.

Full radius preserved. The 0.0 cm radius correction means the bottle sits at the bucket's full 16.7 cm radius, delivering the maximum RCF that the Z366-250 rotor configuration provides.

Swing-out flat-bottom geometry. Distinct from the Z366-06250 6 × 250 mL angle rotor configuration (10,016 × g at 28° angle), the Z366-250 + A250 combination delivers swing-out 250 mL bottle workflow at lower RCF (3,780 × g) but with flat-sediment geometry and the deeper −2 °C cold-chain capability.

Design Architecture

Institutional bulk cell harvest at deep cold-chain temperature. Pelleting cells from 250 mL suspension culture batches at near-freezing temperature for cold-stable downstream-stable preparation; the 4 × 250 mL parallel throughput consolidates institutional prep in one cycle.

Vaccine-development bioprocess prep at 250 mL: vaccine-development workflows that batch 250 mL bottles for intermediate concentration steps with deep cold-chain integrity.

Cold-chain bulk-volume protein workflows. TCA, ammonium-sulfate, organic-precipitation in 250 mL working volume on the deep cold-chain swing-out platform.

Environmental and analytical sample prep at scale. Water, soil-extract, sediment-supernatant prep at 250 mL bottle scale for cold-chain analytical workflows.

Mid-volume institutional clinical-research bulk prep. Pooled patient sample workflows where 4 patient pools batch into 250 mL bottles per cycle.

Cross-format Z366-K core facilities: labs running 250 mL bulk-prep alongside smaller-volume bottle and tube workflows on the same Z366-250 swing-out platform.

Swing-out vs angle 250 mL workflow choice: pick the Z366-250-A250 for swing-out flat-sediment 250 mL workflows on the deep cold-chain chassis; pick the Z366-06250 angle rotor (with 250 mL bottles direct-fit, no insert) when the workflow needs the higher-RCF range (10,016 × g) at the cost of deeper cold-chain temperature.

Ordering & Configuration

4-bucket symmetric loading. All 4 buckets must use matching A250 inserts. Mixed-bucket configurations produce mass asymmetry.

Bottle cap confirmation. All bottles must be securely capped before spinning at 4,500 rpm, at 250 mL working volume, sample loss from a cap failure represents substantial sample volume.

Bottle volume balance. When the 4 bottles carry different sample volumes, pre-balance pairs by total mass before spinning. At 250 mL bottle scale, 1–2 mL volume differences don't materially affect balance, but >5 mL differences do.

Z366-250-A250 ships as 2 inserts. Bottles (250 mL flat-bottom, 62 mm OD) and the Z366-250 bucket are sold separately.

Confirm at quote: bucket pairing (Z366-250 in inventory), chassis pairing (Z366 or Z366-K), bottle format (250 mL flat-bottom, 62 mm OD), pack count (2 packs for full 4-bucket configuration), workflow drivers (deep cold-chain, swing-out geometry, bulk volume), and lead time.

Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts.

Z366 250 mL bottle workflow paths:

- Z366-250-A250 (this product): swing-out 4 × 250 mL on Z366-250 rotor (3,780 × g, −2 °C, swing-out)

- Z366-06250 (host rotor, 250 mL direct-fit): angle 6 × 250 mL on Z366-06250 rotor (10,016 × g, 3 °C, fixed-angle 28°)

Pick the swing-out path when flat-sediment geometry and deepest cold-chain matter; pick the angle path when the higher RCF (>10,000 × g) matters more than swing-out geometry.