The Z327-200-B50 is a direct-fit bucket dimensioned for 2 × 50 mL conical tubes — sized for the Hermle Z327-200-AC swing-out rotor body on the Z327 / Z327-K chassis, sold as a 2-pack. Distinct from the standard bucket-plus-insert approach (Z327-200-BUK + Z327-200-A50, which delivers 4 × 50 mL conical per cycle in lid-compatible mode), the B50 is purpose-built for higher-throughput 50 mL conical workflows — the bucket geometry directly accepts 2 × 50 mL conical tubes without an additional insert. The 4-bucket rotor configuration takes 2 packs (4 B50 buckets) for 8 × 50 mL conical tubes per cycle (400 mL total) — double the throughput of the bucket+insert path. Marked "* only without bucket lid" — non-sealable mode only.
Key Specifications
| SKU | Z327-200-B50 |
| Type | Direct-fit bucket — 2 × 50 mL conical |
| Pack quantity | 2 buckets per SKU (full 4-bucket configuration takes 2 units = 4 buckets) |
| Per-bucket capacity | 2 × 50 mL conical tubes |
| Per-cycle throughput (full configuration) | 8 × 50 mL conical = 400 mL total per cycle |
| Tube format | 50 mL conical Falcon-style (29 mm OD, 116 mm length) |
| Compatible rotor body | Z327-200-AC (4-place swing-out rotor body without buckets) |
| Compatible chassis (assembled) | Z327, Z327-K (refrigerated), and -E voltage variants |
| Max. operating speed (with buckets + tubes) | Per Z327-200-AC spec — 5,000 rpm at 4,136 × g maximum |
| Max. radius | 14.8 cm |
| Geometry | Swing-out (0–90°) — flat sediment surface |
| Bucket lid | Non-lid-compatible — used only without Z327-200-LID (per brochure footnote) |
| List price | $443 (2-pack) |
Direct-Fit Bucket vs Bucket-Plus-Insert — Configuration Choice
The Z327-200 rotor system supports two procurement paths for 50 mL conical workflows on the Z327 / Z327-K chassis:
| Configuration | Per cycle | Mode |
| Z327-200-B50 (this SKU) — direct-fit bucket | 8 × 50 mL conical | Non-sealable |
| Z327-200-BUK + Z327-200-A50 insert | 4 × 50 mL conical | Sealable when paired with Z327-200-LID for BSL-2 + biosafety |
Pick the B50 when higher per-cycle throughput matters (8 vs 4 tubes; 400 mL vs 200 mL total volume) and biosafety containment is not a workflow driver. Pick the BUK + A50 + LID when biosafety containment matters and per-cycle throughput is secondary.
Compatibility & When to Order This SKU
Bucket pairing — Z327-200-AC swing-out rotor body only. Order this SKU when:
- Commissioning a Z327-200-AC for high-throughput 50 mL conical workflow — order 2 units for full 4-bucket configuration (8 tubes/cycle, 400 mL total).
- Adding higher-throughput 50 mL conical capability to an existing Z327 / Z327-K install that currently uses the bucket+insert configuration.
- Replacement buckets if a current set is damaged or worn.
Application Profile
Cold-chain higher-throughput 50 mL clinical-research and biobank prep — patient sample fractionation, aliquot prep, freeze-storage workflows at 8 × 50 mL conical per cycle on the refrigerated chassis (vs 4-tube throughput of the bucket+insert path).
Cell-culture pellet recovery in 50 mL at higher throughput — pelleting cells from 8 parallel 50 mL suspension batches per cycle on the cold-chain swing-out chassis.
Density-gradient prep in 50 mL at higher throughput — Ficoll, Percoll, sucrose-gradient prep at 8 parallel preps per cycle on a refrigerated swing-out chassis.
Cold-chain protein prep at scale — TCA, ammonium-sulfate, organic-precipitation in 50 mL conical at 8 parallel preps with refrigeration.
Institutional clinical-research workflows — when the 4-tube bucket+insert configuration becomes a throughput bottleneck and biosafety containment is not the workflow driver, the B50 doubles per-cycle throughput on the same chassis.
Cross-format Z327-K core facilities — shared cores running both 50 mL conical at high throughput and other tube formats can stock B50 alongside the BUK + insert family for workflow-specific switches.
Ordering & Configuration
4-bucket symmetric loading. All 4 buckets must be installed for symmetric balance at 5,000 rpm.
Per-bucket symmetric loading. Within each bucket, the 2 conical tubes should be balanced — paired by mass before insertion.
Lid removal. Per the brochure footnote, the B50 is used only without the bucket lid — the Z327-200-LID cannot be installed on B50 buckets.
Tube cap confirmation. All conical tubes must be securely capped before spinning at 5,000 rpm.
Z327-200-B50 ships as 2 buckets. 50 mL conical tubes are operator-supplied (commodity Falcon / Corning / equivalent formats).
Required for operation:
- Z327-200-AC rotor body (sold separately) or already in inventory
- For full 4-bucket configuration: order 2 units of this SKU
Confirm at quote: rotor-body pairing (Z327-200-AC in inventory), chassis pairing (Z327 or Z327-K), workflow biosafety driver (B50 is non-sealable; biosafety workflows should use BUK + A50 + LID instead), tube format (50 mL conical Falcon-style), pack count (2 packs for full 4-bucket configuration), and lead time.
Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the buckets.
Z327-200 50 mL conical configuration paths:
- Z327-200-B50 (this product) — direct-fit bucket, 8 tubes/cycle, non-sealable
- Z327-200-BUK + Z327-200-A50 + Z327-200-LID — sealable bucket + insert + lid, 4 tubes/cycle, BSL-2 + capable
Pick the direct-fit B50 for higher non-sealable throughput; pick the BUK + insert + LID combination when biosafety containment is the workflow driver.
