Accuris Broad Range RNA Quantification Kit, 100 Assays

The F1000-RBR-100 is the 100-assay Accuris Broad Range RNA Quantification Kit, the RNA-specific variant of the F1000 fluorometric quantification family. The RNA chemistry uses an RNA-specific fluorescent dye that doesn't bind dsDNA, eliminating the dsDNA-contamination signal that confounds RNA quantification with dsDNA-binding chemistry (e.g., trying to quantify RNA on a Broad Range dsDNA kit). Broad Range covers typical RNA concentration ranges post-extraction; the High Sensitivity sister F1000-RHS-100 covers low-input RNA workflows (cell-free RNA, low-yield extractions).

Key Specifications

For full kit chemistry framework, application context, and Smart-Q family integration, see the F1000-BR-100 parent page (which establishes the F1000 family conventions; same conventions apply to the RNA variants).

Why an RNA-Specific Kit

UV-absorbance methods (NanoDrop / SmartDrop) detect total nucleic acid at A260, both RNA and dsDNA contribute to the absorbance signal, so dsDNA contamination in RNA samples confounds RNA quantification.

Fluorometric methods can be chemistry-specific. The F1000-RBR kit uses an RNA-binding dye that doesn't bind dsDNA, eliminating dsDNA contamination as a quantification confounder. This is critical for:

• NGS RNA-Seq library prep. Accurate RNA quantification before sequencing run normalization, where dsDNA contamination would cause incorrect input-volume calculation
• Low-input RNA workflows. Single-cell RNA-Seq, cell-free RNA, low-yield viral RNA where dsDNA contamination is high relative to target RNA
• DNase-treated RNA samples. Confirming RNA concentration after DNase treatment without dsDNA-contamination signal
• qRT-PCR template quantification. Accurate input quantification for RT-qPCR setup

RNA Family Position

For low-input RNA workflows, see the F1000-RHS sister family. For dsDNA quantification, see the F1000-BR / F1000-HS sister families.

Application Profile

RNA-Seq library prep concentration measurement. NGS RNA-Seq workflows where accurate RNA quantification before pooling matters for run-quality outcomes.

qRT-PCR template quantification. Accurate RNA input quantification for RT-qPCR setup.

Routine RNA quantification post-extraction. Clinical-research and academic labs running RNA extraction workflows.

DNase-treated RNA QC. Confirming RNA concentration after DNase treatment (vs UV-absorbance which would still detect any residual ssDNA + RNA).

Cross-Smart-Q-chassis workflows. Labs running multiple Smart-Q chassis benefit from a single F1000-RBR kit covering all chassis workflows.

Ordering & Configuration

Storage & handling. Per kit instructions: typical fluorometric kit storage at 4 °C protected from light.

Confirm at quote: Smart-Q chassis pairing, RNA workflow context (Broad Range vs the F1000-RHS High Sensitivity for low-input), expected workflow throughput, shipping conditions (cold-chain), and lead time.

Warranty: Per Accuris Life Science Reagents standard kit warranty terms. Confirm at quote.

F1000 RNA quantification kit family:

- F1000-RBR-100 (this product): Broad Range RNA, 100 assays

- F1000-RBR-500: Broad Range RNA, 500 assays (institutional bulk)

- F1000-RHS-100 / -500: High Sensitivity RNA (low-input)

Pick the F1000-RBR for routine RNA concentration ranges; pick the F1000-RHS for low-input RNA workflows.