The Z326-100H-A50R is the 50 mL round-bottom glass insert for the Hermle Z326-100H sealed bucket on the Z327 / Z327-K chassis 4-place swing-out rotor — sold as a 2-pack. Each insert holds 1 × 50 mL round-bottom tube; the 4-bucket rotor configuration takes 2 packs (4 inserts) for full symmetric loading, delivering 4 × 50 mL round-bottom tubes per cycle in hermetically sealed swing-out geometry on a refrigerated chassis. Sister insert to the A50 (50 mL Falcon conical) — both serve the same 50 mL workflow envelope but for different tube geometries.
Key Specifications
| SKU | Z326-100H-A50R |
| Type | Tube insert — 1 × 50 mL round-bottom glass |
| Pack quantity | 2 inserts per SKU (full 4-bucket configuration takes 2 units = 4 inserts) |
| Per-insert capacity | 1 × 50 mL round-bottom tube |
| Per-cycle throughput (full configuration) | 4 × 50 mL round-bottom = 200 mL total per cycle |
| Tube format | 50 mL round-bottom glass (29 mm OD, 90–133 mm length range) |
| Compatible bucket | Z326-100H sealed bucket (with Z326-100H-LID for hermetic seal) |
| Compatible chassis | Z327, Z327-K (refrigerated), and -E voltage variants |
| Max. operating speed (with insert + tube) | Per Z326-100H spec — 5,000 rpm at 4,080 × g maximum |
| Max. radius | 14.6 cm (0.6 cm radius correction with this insert) |
| Temperature at max speed (Z327-K refrigerated) | 2 °C |
| Geometry | Swing-out (0–90°) — flat sediment surface |
| List price | $267 (2-pack) |
Compatibility & When to Order This SKU
Insert pairing — Z326-100H sealed bucket only. Order this SKU when:
- Adding 50 mL round-bottom workflow to an existing Z327 / Z327-K + Z326-100H install — order 2 units for full 4-bucket symmetric loading.
- Glass round-bottom 50 mL workflows when the protocol requires glass for organic-solvent compatibility, repeated autoclave reuse, or the round-bottom geometry over conical.
- Cold-chain BSL-2 + 50 mL round-bottom workflows — when the workflow requires both cold-chain and biosafety containment in 50 mL round-bottom tube format.
- Replacement inserts if a current set is damaged or worn.
Application Profile
Round-bottom vs conical (vs the A50 sister). The A50R holds 50 mL round-bottom glass tubes (90–133 mm length range — accommodates short-format and long-format round-bottom variants). The A50 sister insert holds 50 mL conical Falcon-style tubes (116 mm length). The two formats have different shoulder geometries; using the wrong insert risks tube fit problems and balance issues at the rotor's high-RCF range.
Glass tube workflow compatibility. 50 mL round-bottom glass tubes support workflows requiring glass for organic-solvent compatibility, repeated autoclave reuse, or precise volume measurement (graduated glass tubes).
Hermetically sealed cold-chain workflow. When combined with the Z326-100H-LID, the A50R delivers cold-chain biosafety-contained 50 mL round-bottom workflows.
4-bucket symmetric loading. All 4 buckets must use matching A50R inserts for symmetric balance.
Design Architecture
Cold-chain glass-compatible 50 mL workflows — organic-extraction, chemical-prep, and precipitation workflows in 50 mL round-bottom glass at refrigerated temperature.
BSL-2 + 50 mL round-bottom glass clinical-research prep — clinical-research workflows handling potentially infectious patient samples in 50 mL round-bottom glass that require both cold-chain and biosafety containment.
Cell-culture prep in 50 mL round-bottom glass — when cell-culture protocols specify round-bottom glass for resuspension volume measurement or for solvent-compatibility requirements.
Cold-chain protein prep in glass round-bottom — TCA, ammonium-sulfate, organic-precipitation in 50 mL round-bottom glass on the refrigerated biosafety chassis.
Educational and graduate-level chemistry / biochemistry teaching — workflows specifying round-bottom glass at cold-chain temperature for protocol-fidelity teaching.
Cross-format Z327-K chassis flexibility — labs that run both round-bottom and conical 50 mL workflows benefit from stocking both A50 and A50R inserts on the same bucket.
Ordering & Configuration
4-bucket symmetric loading. All 4 buckets must use matching A50R inserts. Mixed-bucket configurations produce mass asymmetry.
Hermetic-seal engagement. Per Hermle service instructions, the Z326-100H-LID must be fully engaged on each bucket before each spin to maintain the hermetic envelope at 4,080 × g.
Tube cap confirmation. All round-bottom tubes must be capped securely before spinning at 5,000 rpm.
Z326-100H-A50R ships as 2 inserts. 50 mL round-bottom glass tubes are operator-supplied. The Z326-100H bucket and Z326-100H-LID are sold separately.
Confirm at quote: bucket pairing (Z326-100H + Z326-100H-LID in inventory), chassis pairing (Z327 or Z327-K), tube format (50 mL round-bottom glass; A50 for conical), workflow drivers (cold-chain, biosafety, glass tube compatibility), pack count (2 packs for full 4-bucket configuration), and lead time.
Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts. Hermetic-seal interface is a planned-inspection item.
Z326-100H 50 mL insert pair (Z327 / Z327-K chassis):
- Z326-100H-A50R (this product) — 50 mL round-bottom glass, sealable
- Z326-100H-A50 — 50 mL Falcon conical, sealable
Match the insert to the workflow's tube geometry. Both deliver 4-tube parallel throughput on the full 4-bucket configuration with cold-chain and biosafety containment when the bucket lid is engaged.
