The Z496-1L-A500S is the 500 mL flat-bottom bottle insert for the Hermle Z496-1000-1L round buckets on the Z496-1000-AC 4-place swing-out rotor (Z496-K chassis), sold as a 2-pack. Each insert holds 1 × 500 mL flat-bottom bottle (69.5 mm OD); the 4-bucket rotor configuration takes 2 packs (4 inserts) for full symmetric loading, delivering 4 × 500 mL bottles per cycle (2 L total) at the round bucket's higher 5,203 × g max RCF on the cold-chain swing-out chassis. The S suffix denotes the standard 500 mL flat-bottle envelope; distinct from the Z496-1000-A500C insert (500 mL Corning conical with cross-rotor pairing flag).
Key Specifications
| SKU | Z496-1L-A500S |
| Type | Bottle insert (1 × 500 mL flat-bottom) |
| Pack quantity | 2 inserts per SKU (full 4-bucket configuration takes 2 units = 4 inserts) |
| Per-insert capacity | 1 × 500 mL flat-bottom bottle |
| Per-cycle throughput (full configuration) | 4 × 500 mL = 2,000 mL total per cycle |
| Bottle format | 500 mL flat-bottom (69.5 mm OD) |
| Compatible bucket | Z496-1000-1L (1,000 mL round bucket) |
| Compatible rotor | Z496-1000-AC (4-place swing-out rotor body) |
| Compatible chassis (assembled) | Z496-K (Floor-Standing, 230 V) and Z496-K-UC (Underbench, 230 V) |
| Max. operating speed (with insert + bottle) | Per Z496-1000-1L spec (4,600 rpm at 5,203 × g maximum) |
| Max. radius | 22 cm |
| Temperature at max speed (Z496-K refrigerated) | 9 °C |
| Geometry | Swing-out (0–90°): flat sediment surface |
| List price | $572 (2-pack) |
Compatibility & When to Order This SKU
Insert pairing: Z496-1000-1L round bucket only. Order this SKU when:
- Adding 500 mL flat-bottom bottle workflow at high RCF to a Z496-K + Z496-1000-1L install. Order 2 units for full 4-bucket symmetric loading (2 L total per cycle).
- High-RCF cold-chain bulk-volume bottle prep. When the workflow needs the round bucket's 5,203 × g range with cold-chain integrity in 500 mL flat-bottom format.
- Mixed Z496-K round-bucket workflow consolidation: when the lab uses the round bucket for 500 mL flat-bottom workflows alongside other Z496-1L-A* insert formats.
- Replacement inserts if a current set is damaged or worn.
A500S vs A500C: Flat-Bottom vs Cross-Rotor Conical
The Z496-K chassis offers two 500 mL bottle insert paths:
| Insert | Bottle envelope | Rotor pairing | Cross-rotor dependency |
| Z496-1L-A500S (this SKU) | 500 mL flat-bottom (69.5 mm OD) | Z496-1000-1L round bucket | None (standalone insert) |
| Z496-1000-A500C | 500 mL Corning conical | Z496-1000-RBUK rectangular bucket | * Cross-rotor pairing with Z446-750-A500 (per universe label) |
Pick the A500S when the workflow uses standard 500 mL flat-bottom bottles on the Z496-K round-bucket platform, no cross-rotor dependency, full standalone procurement. Pick the A500C when the workflow specifically uses Corning 500 mL conical bottles and the cross-rotor coordination with Z446-750-A500 is part of the procurement.
Application Profile
4-bucket symmetric loading. All 4 round buckets must use matching A500S inserts for symmetric balance.
Bottle format precision. The A500S envelope is dimensioned for the 500 mL flat-bottom bottle with 69.5 mm OD specifically. Bottles outside this dimensional range may not seat properly; verify dimensions at order if using non-standard bottle suppliers.
Design Architecture
High-RCF cold-chain bulk-volume cell harvest in 500 mL flat-bottom: pelleting cells from 4 × 500 mL flat-bottom suspension culture batches per cycle on the round-bucket cold-chain platform with the higher 5,203 × g RCF range.
Vaccine-development bioprocess prep at 500 mL flat-bottom: vaccine-development workflows that batch 500 mL flat-bottom bottles for intermediate concentration steps with cold-chain integrity.
Recombinant-protein bulk prep workflows in 500 mL flat-bottom: recombinant-protein expression and harvest in 500 mL flat-bottom bottle format on the high-RCF cold-chain swing-out chassis.
Cold-chain bulk-volume protein workflows. TCA, ammonium-sulfate, organic-precipitation in 500 mL working volume with flat-base pellet distribution.
Environmental and analytical bulk sample prep at 500 mL flat-bottom: water, soil-extract, sediment-supernatant prep at 500 mL flat-bottom bottle scale on the high-RCF cold-chain platform.
Mixed-format Z496-K core facilities: labs running 500 mL flat-bottom alongside other Z496-1L-A* insert formats benefit from the cross-format insert family on the round bucket.
Ordering & Configuration
4-bucket symmetric loading. All 4 round buckets must use matching A500S inserts. Mixed-bucket configurations produce mass asymmetry.
Bottle cap confirmation. All bottles must be securely capped before spinning at 4,600 rpm, at 500 mL working volume and high RCF, cap integrity is critical.
Bottle volume balance. When the 4 bottles carry different sample volumes, pre-balance pairs by total mass before spinning.
Z496-1L-A500S ships as 2 inserts. Bottles (500 mL flat-bottom, 69.5 mm OD) are operator-supplied. The Z496-1000-1L round buckets are sold separately.
Confirm at quote: bucket pairing (Z496-1000-1L round buckets in inventory), chassis pairing (Z496-K or Z496-K-UC), bottle format (500 mL flat-bottom, 69.5 mm OD; verify dimensions if using non-standard bottles), pack count (2 packs for full 4-bucket configuration = 2 L total per cycle), and lead time.
Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts.
Z496-K 500 mL bottle insert paths:
- Z496-1L-A500S (this product): 500 mL flat-bottom on Z496-1000-1L round bucket (standalone, high RCF)
- Z496-1000-A500: 500 mL bottle (Hermle C1500) on Z496-1000-RBUK rectangular bucket
- Z496-1000-A500C: 500 mL Corning conical on Z496-1000-RBUK (with Z446-750-A500 cross-rotor pairing flag)
Match the insert to the bottle envelope (flat-bottom vs Hermle C1500 vs Corning conical) and bucket geometry (round vs rectangular).
