The Z496-1L-A500S is the 500 mL flat-bottom bottle insert for the Hermle Z496-1000-1L round buckets on the Z496-1000-AC 4-place swing-out rotor (Z496-K chassis) — sold as a 2-pack. Each insert holds 1 × 500 mL flat-bottom bottle (69.5 mm OD); the 4-bucket rotor configuration takes 2 packs (4 inserts) for full symmetric loading, delivering 4 × 500 mL bottles per cycle (2 L total) at the round bucket's higher 5,203 × g max RCF on the cold-chain swing-out chassis. The S suffix denotes the standard 500 mL flat-bottle envelope; distinct from the Z496-1000-A500C insert (500 mL Corning conical with cross-rotor pairing flag).
Key Specifications
| SKU | Z496-1L-A500S |
| Type | Bottle insert — 1 × 500 mL flat-bottom |
| Pack quantity | 2 inserts per SKU (full 4-bucket configuration takes 2 units = 4 inserts) |
| Per-insert capacity | 1 × 500 mL flat-bottom bottle |
| Per-cycle throughput (full configuration) | 4 × 500 mL = 2,000 mL total per cycle |
| Bottle format | 500 mL flat-bottom (69.5 mm OD) |
| Compatible bucket | Z496-1000-1L (1,000 mL round bucket) |
| Compatible rotor | Z496-1000-AC (4-place swing-out rotor body) |
| Compatible chassis (assembled) | Z496-K (Floor-Standing, 230 V) and Z496-K-UC (Underbench, 230 V) |
| Max. operating speed (with insert + bottle) | Per Z496-1000-1L spec — 4,600 rpm at 5,203 × g maximum |
| Max. radius | 22 cm |
| Temperature at max speed (Z496-K refrigerated) | 9 °C |
| Geometry | Swing-out (0–90°) — flat sediment surface |
| List price | $572 (2-pack) |
Compatibility & When to Order This SKU
Insert pairing — Z496-1000-1L round bucket only. Order this SKU when:
- Adding 500 mL flat-bottom bottle workflow at high RCF to a Z496-K + Z496-1000-1L install — order 2 units for full 4-bucket symmetric loading (2 L total per cycle).
- High-RCF cold-chain bulk-volume bottle prep — when the workflow needs the round bucket's 5,203 × g range with cold-chain integrity in 500 mL flat-bottom format.
- Mixed Z496-K round-bucket workflow consolidation — when the lab uses the round bucket for 500 mL flat-bottom workflows alongside other Z496-1L-A* insert formats.
- Replacement inserts if a current set is damaged or worn.
A500S vs A500C — Flat-Bottom vs Cross-Rotor Conical
The Z496-K chassis offers two 500 mL bottle insert paths:
| Insert | Bottle envelope | Rotor pairing | Cross-rotor dependency |
| Z496-1L-A500S (this SKU) | 500 mL flat-bottom (69.5 mm OD) | Z496-1000-1L round bucket | None (standalone insert) |
| Z496-1000-A500C | 500 mL Corning conical | Z496-1000-RBUK rectangular bucket | * Cross-rotor pairing with Z446-750-A500 (per universe label) |
Pick the A500S when the workflow uses standard 500 mL flat-bottom bottles on the Z496-K round-bucket platform — no cross-rotor dependency, full standalone procurement. Pick the A500C when the workflow specifically uses Corning 500 mL conical bottles and the cross-rotor coordination with Z446-750-A500 is part of the procurement.
Application Profile
4-bucket symmetric loading. All 4 round buckets must use matching A500S inserts for symmetric balance.
Bottle format precision. The A500S envelope is dimensioned for the 500 mL flat-bottom bottle with 69.5 mm OD specifically. Bottles outside this dimensional range may not seat properly; verify dimensions at order if using non-standard bottle suppliers.
Design Architecture
High-RCF cold-chain bulk-volume cell harvest in 500 mL flat-bottom — pelleting cells from 4 × 500 mL flat-bottom suspension culture batches per cycle on the round-bucket cold-chain platform with the higher 5,203 × g RCF range.
Vaccine-development bioprocess prep at 500 mL flat-bottom — vaccine-development workflows that batch 500 mL flat-bottom bottles for intermediate concentration steps with cold-chain integrity.
Recombinant-protein bulk prep workflows in 500 mL flat-bottom — recombinant-protein expression and harvest in 500 mL flat-bottom bottle format on the high-RCF cold-chain swing-out chassis.
Cold-chain bulk-volume protein workflows — TCA, ammonium-sulfate, organic-precipitation in 500 mL working volume with flat-base pellet distribution.
Environmental and analytical bulk sample prep at 500 mL flat-bottom — water, soil-extract, sediment-supernatant prep at 500 mL flat-bottom bottle scale on the high-RCF cold-chain platform.
Mixed-format Z496-K core facilities — labs running 500 mL flat-bottom alongside other Z496-1L-A* insert formats benefit from the cross-format insert family on the round bucket.
Ordering & Configuration
4-bucket symmetric loading. All 4 round buckets must use matching A500S inserts. Mixed-bucket configurations produce mass asymmetry.
Bottle cap confirmation. All bottles must be securely capped before spinning at 4,600 rpm — at 500 mL working volume and high RCF, cap integrity is critical.
Bottle volume balance. When the 4 bottles carry different sample volumes, pre-balance pairs by total mass before spinning.
Z496-1L-A500S ships as 2 inserts. Bottles (500 mL flat-bottom, 69.5 mm OD) are operator-supplied. The Z496-1000-1L round buckets are sold separately.
Confirm at quote: bucket pairing (Z496-1000-1L round buckets in inventory), chassis pairing (Z496-K or Z496-K-UC), bottle format (500 mL flat-bottom, 69.5 mm OD — verify dimensions if using non-standard bottles), pack count (2 packs for full 4-bucket configuration = 2 L total per cycle), and lead time.
Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts.
Z496-K 500 mL bottle insert paths:
- Z496-1L-A500S (this product) — 500 mL flat-bottom on Z496-1000-1L round bucket (standalone, high RCF)
- Z496-1000-A500 — 500 mL bottle (Hermle C1500) on Z496-1000-RBUK rectangular bucket
- Z496-1000-A500C — 500 mL Corning conical on Z496-1000-RBUK (with Z446-750-A500 cross-rotor pairing flag)
Match the insert to the bottle envelope (flat-bottom vs Hermle C1500 vs Corning conical) and bucket geometry (round vs rectangular).
