The Z366-250-B250 is a direct-fit bucket dimensioned for 1 × 250 mL conical bottle — sized for the Hermle Z366-250-HC swing-out rotor on the Z366 / Z366-K chassis 4-place configuration, sold as a 2-pack. Distinct from the bucket-plus-insert approach (Z366-250 + Z366-250-A250 insert delivers 4 × 250 mL bottle per cycle), the B250 direct-fit bucket on the HC rotor variant delivers the same per-cycle throughput (4 × 250 mL = 1 L total) but with a dedicated bottle-format bucket geometry and the deep cold-chain capability of the Z366-K chassis (−2 °C at max speed).
Key Specifications
| SKU | Z366-250-B250 |
| Type | Direct-fit bucket — 1 × 250 mL conical bottle |
| Pack quantity | 2 buckets per SKU (full 4-bucket configuration takes 2 units = 4 buckets) |
| Per-bucket capacity | 1 × 250 mL conical bottle |
| Per-cycle throughput (full configuration) | 4 × 250 mL = 1,000 mL total per cycle |
| Bottle format | 250 mL conical bottle (62 mm OD, 122 mm length) |
| Compatible rotor | Z366-250-HC swing-out rotor (HC = high-capacity variant of Z366-250) |
| Compatible chassis | Z366, Z366-K (refrigerated), and -E voltage variants |
| Max. operating speed | 4,500 rpm at 3,735 × g maximum |
| Max. radius | 16.5 cm (0.0 cm radius correction — full radius preserved) |
| Temperature at max speed (Z366-K refrigerated) | −2 °C |
| Geometry | Swing-out (0–90°) — flat sediment surface |
| List price | $545 (2-pack) |
Direct-Fit Bucket vs Bucket-Plus-Insert — Configuration Choice
The Z366 chassis offers two paths for swing-out 250 mL bottle workflows:
| Configuration | Per cycle | Approach |
| Z366-250-B250 (this SKU) | 4 × 250 mL conical | Direct-fit bucket on Z366-250-HC rotor (dedicated, no insert needed) |
| Z366-250 + Z366-250-A250 insert | 4 × 250 mL flat | Bucket + insert on Z366-250 rotor (bucket holds other inserts too) |
Per-cycle throughput is the same (4 bottles); the choice depends on bottle envelope (B250 is dimensioned for conical 250 mL bottles; A250 is dimensioned for flat-bottom 250 mL bottles) and on whether the lab needs the bucket to serve multiple formats (insert path) or dedicate to 250 mL only (direct-fit path).
Compatibility & When to Order This SKU
Bucket pairing — Z366-250-HC rotor only. Order this SKU when:
- Commissioning a Z366-250-HC for institutional 250 mL conical bottle workflow — order 2 units for full 4-bucket configuration (4 bottles/cycle, 1 L total).
- Adding 250 mL conical workflow to an existing Z366 / Z366-K install with the HC rotor variant.
- Workflows specifying 250 mL conical bottles (vs flat-bottom 250 mL — which uses the A250 insert on the standard Z366-250 rotor).
- Replacement buckets if a current set is damaged or worn.
Application Profile
4-bucket symmetric loading. All 4 buckets must be installed for symmetric balance at 4,500 rpm.
Bottle format precision. The B250 envelope is dimensioned for the 62 mm OD × 122 mm length conical bottle range. Bottles outside this dimensional range may not seat properly; verify dimensions if using non-standard bottle suppliers.
Full radius preserved. The 0.0 cm radius correction means the bottle sits at the bucket's full 16.5 cm radius — delivering the maximum RCF that the Z366-250-HC rotor configuration provides.
Design Architecture
Institutional bulk cell harvest at deep cold-chain temperature — pelleting cells from 250 mL conical bottle suspension culture batches at near-freezing temperature for cold-stable downstream-stable preparation.
Vaccine-development bioprocess prep at 250 mL conical — vaccine-development workflows that batch 250 mL conical bottles for intermediate concentration steps with deep cold-chain integrity.
Cold-chain bulk-volume conical bottle workflows — when the workflow specifies conical bottle geometry (over flat-bottom) for protocol compatibility or sample-recovery ergonomics.
Environmental and analytical sample prep at scale — water, soil-extract, sediment-supernatant prep at 250 mL conical bottle scale for cold-chain analytical workflows.
Recombinant-protein bulk prep workflows — recombinant-protein expression and harvest in 250 mL conical bottle format on the deep cold-chain swing-out chassis.
Cross-format Z366-K / HC core facilities — shared cores running both 250 mL conical (this SKU) and 250 mL flat-bottom (A250) workflows benefit from understanding the bottle-envelope distinction at procurement.
Ordering & Configuration
4-bucket symmetric loading. All 4 buckets must be installed for symmetric balance.
Bottle cap confirmation. All bottles must be securely capped before spinning at 4,500 rpm — at 250 mL working volume, sample loss from a cap failure represents substantial sample volume.
Bottle volume balance. When the 4 bottles carry different sample volumes, pre-balance pairs by total mass before spinning.
Z366-250-B250 ships as 2 buckets. Bottles (250 mL conical, 62 mm OD) are operator-supplied.
Required for operation:
- Z366-250-HC rotor body (sold separately) or already in inventory
- For full 4-bucket configuration: order 2 units of this SKU
Confirm at quote: rotor pairing (Z366-250-HC in inventory), chassis pairing (Z366 or Z366-K), bottle format (250 mL conical, 62 mm OD; flat-bottom 250 mL uses A250 insert on Z366-250 rotor instead), pack count (2 packs for full 4-bucket configuration), and lead time.
Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the buckets.
Z366 250 mL bottle workflow paths:
- Z366-250-B250 (this product) — direct-fit bucket on Z366-250-HC, 250 mL conical
- Z366-250-A250 — insert on Z366-250 bucket, 250 mL flat-bottom
- Z366-06250 — 250 mL angle rotor (10,016 × g, 28°, fixed-angle direct fit)
Pick the swing-out direct-fit B250 for 250 mL conical workflow on the cold-chain HC platform; pick the A250 insert for 250 mL flat-bottom; pick the angle rotor when high RCF (>10,000 × g) matters more than swing-out geometry.
