250 mL Conical Bottle Insert for Z446-04500 (2/pk)

The Z446-500-A250 is the 250 mL conical bottle insert for the Hermle Z446-04500 4 × 500 mL angle rotor on the Z446 / Z446-K chassis — sold as a 2-pack. Each insert holds 1 × 250 mL conical bottle; the 4-position rotor takes 2 packs (4 inserts) for full symmetric loading, delivering 4 × 250 mL conical bottles per cycle (1 L total) at the host rotor's high-RCF range (10,367 × g). The engineered bottle is Hermle's C1250 250 mL conical centrifuge bottle, sold separately. Sister insert to the Z446-500-A250F (flat-bottom 250 mL bottle) — same volume, different bottle envelope geometry.

Key Specifications

Compatibility & When to Order This SKU

Insert pairing — Z446-04500 500 mL angle rotor only. Order this SKU when:

1. Adding 250 mL conical bottle workflow to a Z446-04500 install — order 2 units for full 4-position symmetric loading (1 L total per cycle).
2. High-RCF cold-chain bulk-volume conical bottle prep — when the workflow needs 250 mL conical bottle format with cold-chain integrity at >10,000 × g.
3. Mixed 500 / 250 mL workflow consolidation — when the chassis primarily runs 500 mL bottle workflows but occasional 250 mL conical processing arises.
4. Replacement inserts if a current set is damaged or worn.

Conical vs Flat-Bottom 250 mL — Why Two Insert SKUs

The Z446-500 250 mL insert pair serves the same volume but different bottle envelopes:

The conical bottle (Hermle C1250) has a tapered base that supports cell-pellet recovery with the pellet concentrating at the conical tip. The flat-bottom bottle has a flat base that distributes pellet across a larger surface area. Pick the A250 when the workflow specifies conical bottle geometry (Hermle C1250 or compatible); pick the A250F when flat-bottom geometry is required.

Application Profile

Bulk-volume cell harvest in 250 mL conical — pelleting cells from 250 mL suspension culture batches at 4 × 250 mL parallel throughput; the conical bottle geometry concentrates the pellet at the tip for efficient sample recovery.

Vaccine-development bioprocess prep at 250 mL conical — vaccine-development workflows that batch 4 × 250 mL bottles per cycle for intermediate concentration steps with cold-chain integrity.

High-RCF cold-chain bulk-volume protein workflows in 250 mL conical — TCA, ammonium-sulfate, organic-precipitation in 250 mL working volume on the cold-chain refrigerated chassis with the conical-tip pellet concentration.

Recombinant-protein bulk prep workflows — recombinant-protein expression and harvest in 250 mL conical bottle format at >10,000 × g.

Environmental and analytical bulk sample prep at 250 mL conical — water, soil-extract, sediment-supernatant prep at 250 mL conical bottle scale.

Cross-format Z446-K core facilities — labs running 250 mL conical alongside other Z446-04500 insert formats benefit from stocking both A250 (conical) and A250F (flat) variants for tube-format flexibility.

Ordering & Configuration

4-position symmetric loading. All 4 positions on the host rotor must use matching A250 inserts for symmetric balance at 8,000 rpm.

Bottle cap confirmation. All bottles must be securely capped before spinning at 8,000 rpm — at 250 mL working volume and high RCF, cap integrity is critical.

Bottle volume balance. When the 4 bottles carry different sample volumes, pre-balance pairs by total mass before spinning.

Z446-500-A250 ships as 2 inserts. Bottles (Hermle C1250 250 mL conical) are sold separately.

Confirm at quote: host rotor (Z446-04500 in inventory), chassis pairing (Z446 or Z446-K), bottle format (250 mL conical, Hermle C1250 — verify dimensions if using third-party bottles), pack count (2 packs for full 4-position configuration), and lead time.

Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts.

Z446-500 250 mL insert pair (Z446-04500 4 × 500 mL angle rotor):

- Z446-500-A250 (this product) — 250 mL conical (Hermle C1250)

- Z446-500-A250F — 250 mL flat-bottom

Match the insert to the workflow's bottle envelope geometry — conical for tip-concentrated pellet recovery, flat-bottom for distributed pellet workflows.