The Z496-1L-A250F is the 250 mL conical bottle insert for the Hermle Z496-1000-1L round buckets on the Z496-1000-AC 4-place swing-out rotor (Z496-K chassis) — sold as a 2-pack. Each insert holds 1 × 250 mL conical bottle; the 4-bucket rotor configuration takes 2 packs (4 inserts) for full symmetric loading, delivering 4 × 250 mL conical bottles per cycle (1 L total) at the round bucket's higher 5,203 × g max RCF on the cold-chain swing-out chassis.
Naming-convention note: Per the universe price-list label, this SKU is "250ml conical bottle insert" — the F suffix here indicates the conical-bottle-format-specific variant. Confirm bottle envelope at order; the F suffix conventionally denotes "flat" on some Hermle adapter families and "format-specific" on others (see also FQ-008 in the fred_followup_queue regarding the Z446 family naming-convention reversal).
Key Specifications
| SKU | Z496-1L-A250F |
| Type | Bottle insert — 1 × 250 mL conical |
| Pack quantity | 2 inserts per SKU (full 4-bucket configuration takes 2 units = 4 inserts) |
| Per-insert capacity | 1 × 250 mL conical bottle |
| Per-cycle throughput (full configuration) | 4 × 250 mL = 1,000 mL total per cycle |
| Bottle format | 250 mL conical bottle (per universe label) |
| Compatible bucket | Z496-1000-1L (1,000 mL round bucket) |
| Compatible rotor | Z496-1000-AC (4-place swing-out rotor body) |
| Compatible chassis (assembled) | Z496-K (Floor-Standing, 230 V) and Z496-K-UC (Underbench, 230 V) |
| Max. operating speed (with insert + bottle) | Per Z496-1000-1L spec — 4,600 rpm at 5,203 × g maximum |
| Max. radius | 22 cm |
| Temperature at max speed (Z496-K refrigerated) | 9 °C |
| Geometry | Swing-out (0–90°) — flat sediment surface |
| List price | $572 (2-pack) |
Compatibility & When to Order This SKU
Insert pairing — Z496-1000-1L round bucket only. Order this SKU when:
- Adding 250 mL conical bottle workflow at high RCF to a Z496-K + Z496-1000-1L install — order 2 units for full 4-bucket symmetric loading (1 L total per cycle).
- High-RCF cold-chain 250 mL conical bottle prep — when the workflow needs the round bucket's 5,203 × g range with cold-chain integrity in 250 mL conical format.
- Mixed round-bucket Z496-K workflow consolidation — when the lab uses the round bucket for 250 mL conical workflows alongside other Z496-1L-A* insert formats.
- Replacement inserts if a current set is damaged or worn.
Application Profile
4-bucket symmetric loading. All 4 round buckets must use matching A250F inserts for symmetric balance.
Bottle format precision. Confirm bottle envelope dimensions match the insert spec at order. Per Hermle catalog convention for the Z496-1L family, the A250F is the conical-bottle variant; if the workflow uses flat-bottom 250 mL bottles, verify that this insert is the right pick (vs. a separate flat-bottom variant if available).
Design Architecture
High-RCF cold-chain 250 mL conical bottle prep — vaccine-development, recombinant-protein, and bioprocess workflows that batch 4 × 250 mL conical bottles per cycle at high RCF and cold-chain temperature on the round-bucket swing-out chassis.
Mid-volume cell harvest in 250 mL conical at high RCF — pelleting cells from 4 parallel 250 mL conical bottle suspension batches per cycle with the round bucket's 5,203 × g range for tip-concentrated pellet recovery.
Cold-chain bioprocess intermediate prep at 250 mL conical — vaccine-development workflows that batch 250 mL conical bottles for intermediate concentration steps with high RCF.
Recombinant-protein bulk prep workflows in 250 mL conical — recombinant-protein expression and harvest in 250 mL conical bottle format on the high-RCF cold-chain swing-out chassis.
Mixed-format Z496-K core facilities — labs running 250 mL conical alongside other Z496-1L-A* insert formats benefit from the cross-format insert family on the round bucket.
Ordering & Configuration
4-bucket symmetric loading. All 4 round buckets must use matching A250F inserts. Mixed-bucket configurations produce mass asymmetry.
Bottle cap confirmation. All bottles must be securely capped before spinning at 4,600 rpm.
Bottle volume balance. When the 4 bottles carry different sample volumes, pre-balance pairs by total mass before spinning.
Z496-1L-A250F ships as 2 inserts. Bottles (250 mL conical) are operator-supplied. The Z496-1000-1L round buckets are sold separately.
Confirm at quote: bucket pairing (Z496-1000-1L round buckets in inventory), chassis pairing (Z496-K or Z496-K-UC), bottle format (250 mL conical — confirm envelope dimensions if F suffix interpretation matters for bottle source), pack count (2 packs for full 4-bucket configuration), and lead time.
Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts.
Z496-1L round-bucket insert family (Z496-1000-1L on Z496-K chassis):
- Z496-1L-A250F (this product) — 250 mL conical bottle insert
- Z496-1L-A500S — 500 mL flat bottle (69.5 mm dia) insert
- Z496-1L-A15 — 12 × 15 mL conical (48/cycle)
Match the insert to the workflow's bottle / tube format. The Z496-1L family delivers higher RCF (5,203 × g) than the rectangular-bucket family on the same chassis.
