The Z446-750-A250F is the 250 mL conical bottle insert for the Hermle Z446-750-BUK round bucket on the Z446-750-AC 4-place swing-out rotor (Z446 / Z446-K chassis) — sold as a 2-pack. Each insert holds 1 × 250 mL conical bottle; the 4-bucket rotor configuration takes 2 packs (4 inserts) for full symmetric loading, delivering 4 × 250 mL conical bottles per cycle (1 L total) in swing-out geometry. Per the universe price-list label, this insert is dimensioned for the conical 250 mL bottle envelope; the sister Z446-750-A250 SKU is dimensioned for the flat-bottom 250 mL bottle envelope.
Naming-convention note: On the Z446-750 swing-out side, the F suffix denotes Falcon (Corning's conical bottle brand), so A250F = Falcon-conical (this SKU) and A250 = flat-bottom. Per Fred 2026-05-05. The Z446-500 angle family uses the F suffix differently (per universe, A250F = flat on the angle side). The two families use the F suffix structurally differently — confirm envelope by SKU at order, not by suffix.
Key Specifications
| SKU | Z446-750-A250F |
| Type | Bottle insert — 1 × 250 mL conical |
| Pack quantity | 2 inserts per SKU (full 4-bucket configuration takes 2 units = 4 inserts) |
| Per-insert capacity | 1 × 250 mL conical bottle |
| Per-cycle throughput (full configuration) | 4 × 250 mL = 1,000 mL total per cycle |
| Bottle format | 250 mL Falcon-conical (Corning conical brand; F suffix = Falcon per Fred 2026-05-05) |
| Compatible bucket | Z446-750-BUK (round bucket; with Z446-750-LID PC-Screw cap for sealable mode) |
| Compatible rotor | Z446-750-AC (4-place swing-out rotor body) |
| Compatible chassis | Z446, Z446-K (refrigerated), and -E voltage variants |
| Max. operating speed (with insert + bottle) | Per Z446-750-AC spec — 4,000/4,500 rpm at 3,434–4,346 × g maximum |
| Max. radius | 19.2 cm |
| Temperature at max speed (refrigerated chassis) | 4 °C |
| Geometry | Swing-out (0–90°) — flat sediment surface |
| List price | $572 (2-pack) |
Compatibility & When to Order This SKU
Insert pairing — Z446-750-BUK round bucket only. Order this SKU when:
- Adding 250 mL conical bottle workflow on the swing-out platform — order 2 units for full 4-bucket symmetric loading (1 L total per cycle).
- Cold-chain swing-out 250 mL conical bottle prep — when the workflow benefits from swing-out flat-sediment geometry AND uses conical bottle envelope for tip-concentrated pellet recovery.
- Mixed Z446-750 swing-out workflow consolidation — when the lab uses the same chassis for clinical-blood prep at smaller volumes AND occasional 250 mL conical bottle workflows.
- Optional BSL-2 + workflow with the Z446-750-LID engaged.
- Replacement inserts if a current set is damaged or worn.
Conical vs Flat 250 mL on Z446-750 — F Suffix Means Falcon, Not Flat
The Z446 / Z446-K chassis offers four 250 mL bottle insert SKUs across two rotor systems with inconsistent naming conventions between the angle-rotor and swing-out families:
| SKU | Rotor | Bottle envelope (per universe label) |
| Z446-750-A250F (this SKU) | Swing-out (Z446-750-AC) | 250 mL conical |
| Z446-750-A250 | Swing-out (Z446-750-AC) | 250 mL flat-bottom |
| Z446-500-A250 | Angle (Z446-04500) | 250 mL conical (Hermle C1250) |
| Z446-500-A250F | Angle (Z446-04500) | 250 mL flat-bottom |
On the Z446-750 swing-out side, the F suffix denotes Falcon (Corning's conical bottle brand) per Fred 2026-05-05. The Z446-500 angle family uses the F suffix differently (per universe label, A250F = flat-bottom on the angle side). The two rotor families assign the F suffix on different bases — confirm envelope by SKU at order, not by inferring meaning from the F suffix.
Application Profile
Cold-chain swing-out conical 250 mL bottle prep — vaccine-development, recombinant-protein, and bioprocess workflows that batch 4 × 250 mL conical bottles per cycle with cold-chain integrity and flat-sediment geometry plus tip-concentrated pellet recovery.
Mid-volume cell harvest in 250 mL conical — pelleting cells from suspension culture batches; the conical bottle geometry concentrates the pellet at the tip for efficient sample recovery.
Vaccine-development bioprocess prep at 250 mL conical — vaccine-development workflows that batch 250 mL conical bottles for intermediate concentration steps with the conical-tip pellet concentration on the swing-out platform.
Recombinant-protein bulk prep workflows in 250 mL conical — recombinant-protein expression and harvest in 250 mL conical bottle format on the swing-out cold-chain chassis.
Cold-chain protein prep workflows in 250 mL conical — TCA, ammonium-sulfate, organic-precipitation in 250 mL working volume with conical-tip pellet recovery.
Cross-format Z446-K core facilities — labs running 250 mL conical alongside other Z446-750 insert formats benefit from the cross-format insert family.
Ordering & Configuration
4-bucket symmetric loading. All 4 buckets must use matching A250F inserts for symmetric balance.
Bottle cap confirmation. All bottles must be capped securely before spinning at 4,500 rpm.
Bottle volume balance. When the 4 bottles carry different sample volumes, pre-balance pairs by total mass before spinning.
Optional hermetic-seal engagement. When using the Z446-750-LID for biosafety workflows, confirm the lid is fully threaded onto each bucket before each spin.
Z446-750-A250F ships as 2 inserts. Bottles (250 mL conical) are operator-supplied.
Confirm bottle envelope at order — this is the Falcon-conical 250 mL bottle insert (F suffix = Falcon per Fred 2026-05-05; the sister A250 is flat-bottom). Confirm at quote: bucket pairing (Z446-750-BUK in inventory; Z446-750-LID if biosafety required), chassis pairing (Z446 or Z446-K) and power configuration, bottle format (250 mL Falcon-conical for this A250F), pack count (2 packs for full 4-bucket configuration), and lead time.
Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts.
Z446-750 250 mL insert pair (Z446-750-AC swing-out rotor):
- Z446-750-A250F (this product) — 250 mL Falcon-conical bottle (F = Falcon per Fred 2026-05-05)
- Z446-750-A250 — 250 mL flat-bottom bottle
The two rotor families (Z446-750 swing-out and Z446-500 angle) assign the F suffix on different bases. Confirm envelope by SKU at order.
