250 mL Flat-Bottom Bottle Insert for Z446-04500 (2/pk)

The Z446-500-A250F is the 250 mL flat-bottom bottle insert for the Hermle Z446-04500 4 × 500 mL angle rotor on the Z446 / Z446-K chassis, sold as a 2-pack. Each insert holds 1 × 250 mL flat-bottom bottle; the 4-position rotor takes 2 packs (4 inserts) for full symmetric loading, delivering 4 × 250 mL bottles per cycle (1 L total) at the host rotor's high-RCF range (10,367 × g). The F suffix denotes flat-bottom envelope (vs the conical envelope of the Z446-500-A250 sister insert), same volume, different bottle geometry for workflows requiring flat-base bottle format.

Key Specifications

Compatibility & When to Order This SKU

Insert pairing: Z446-04500 500 mL angle rotor only. Order this SKU when:

1. Adding 250 mL flat-bottom bottle workflow to a Z446-04500 install. Order 2 units for full 4-position symmetric loading (1 L total per cycle).
2. High-RCF cold-chain bulk-volume flat-bottom bottle prep. When the workflow specifies flat-base bottle geometry (over conical) for cell-pellet distribution, organic-extraction phase separation, or other protocol-specific reasons.
3. Mixed 500 / 250 mL workflow consolidation: when the chassis primarily runs 500 mL bottle workflows but occasional 250 mL flat-bottom processing arises.
4. Replacement inserts if a current set is damaged or worn.

Conical vs Flat-Bottom 250 mL: Why Two Insert SKUs

The Z446-500 250 mL insert pair serves the same volume but different bottle envelopes:

The flat-bottom bottle distributes pellet across a larger surface area (useful for organic-extraction phase separation, distributed cell-monolayer pellet workflows). The conical bottle (Hermle C1250) concentrates pellet at the tip (useful for efficient sample recovery from small cell pellets). Pick the A250F when flat-base geometry is the protocol-specified format.

Application Profile

Bulk-volume cell harvest in 250 mL flat-bottom: pelleting cells from 250 mL suspension culture batches at 4 × 250 mL parallel throughput; the flat-bottom bottle distributes the pellet across a wider base for protocols that resuspend the pellet in larger working volume.

Organic-extraction phase separation in 250 mL flat-bottom glass: when the workflow includes phase separation of aqueous and organic phases in 250 mL working volume and the flat-base geometry supports clean phase boundaries.

Cold-chain mid-volume protein-prep workflows in flat-bottom format. TCA, ammonium-sulfate, organic-precipitation in 250 mL working volume on the cold-chain refrigerated chassis with flat-base pellet distribution.

Vaccine-development intermediate prep at 250 mL flat-bottom: vaccine-development workflows that batch 250 mL flat-bottom bottles for intermediate concentration steps where the flat-base format is the protocol standard.

Environmental and analytical bulk sample prep at 250 mL flat-bottom: water, soil-extract, sediment-supernatant prep at 250 mL flat-bottom bottle scale.

Cross-format Z446-K core facilities: labs running both 250 mL flat-bottom (this SKU) and 250 mL conical (A250) workflows benefit from stocking both insert variants.

Ordering & Configuration

4-position symmetric loading. All 4 positions on the host rotor must use matching A250F inserts for symmetric balance at 8,000 rpm.

Bottle cap confirmation. All bottles must be securely capped before spinning at 8,000 rpm, at 250 mL working volume and high RCF, cap integrity is critical.

Bottle volume balance. When the 4 bottles carry different sample volumes, pre-balance pairs by total mass before spinning.

Z446-500-A250F ships as 2 inserts. Bottles (250 mL flat-bottom, 62 mm OD) are operator-supplied.

Confirm at quote: host rotor (Z446-04500 in inventory), chassis pairing (Z446 or Z446-K), bottle format (250 mL flat-bottom, 62 mm OD; for conical 250 mL use A250 insert with Hermle C1250 bottle), pack count (2 packs for full 4-position configuration), and lead time.

Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts.

Z446-500 250 mL insert pair (Z446-04500 4 × 500 mL angle rotor):

- Z446-500-A250F (this product): 250 mL flat-bottom (122 mm length)

- Z446-500-A250: 250 mL conical (159 mm length, Hermle C1250 bottle)

Match the insert to the workflow's bottle envelope geometry, flat-base for distributed pellet workflows, conical for tip-concentrated pellet recovery.