175/225 mL Conical Bottle Insert for Z446-750-BUK (2/pk)

The Z446-750-A225 is the 175/225 mL conical bottle insert for the Hermle Z446-750-BUK round bucket on the Z446-750-AC 4-place swing-out rotor (Z446 / Z446-K chassis), sold as a 2-pack. Each insert holds 1 × 175 mL or 225 mL conical bottle; the 4-bucket rotor configuration takes 2 packs (4 inserts) for full symmetric loading, delivering 4 × 175/225 mL bottles per cycle (700 mL or 900 mL total) in swing-out geometry. The engineered bottles are Hermle's C0175 (175 mL) and C0225 (225 mL) conical centrifuge bottles, sold separately.

Distinct from the Z446-500-A225C insert (same bottle envelope on the Z446-04500 angle rotor at 10,367 × g). Pick the Z446-750-A225 swing-out path when flat-sediment geometry matters; pick the angle path when the higher RCF range is required.

Key Specifications

Compatibility & When to Order This SKU

Insert pairing: Z446-750-BUK round bucket only. Order this SKU when:

1. Adding 175 or 225 mL conical bottle workflow on the swing-out platform: order 2 units for full 4-bucket symmetric loading (700 / 900 mL total per cycle).
2. Cold-chain swing-out 175-225 mL bottle prep: when the workflow benefits from swing-out flat-sediment geometry (vs the angled pellet of the Z446-04500 angle rotor) for downstream supernatant aspiration in 175/225 mL bottles.
3. Mixed Z446-750 swing-out workflow consolidation: when the lab uses the same chassis for clinical-blood prep at smaller volumes AND occasional 175-225 mL bottle workflows.
4. Optional BSL-2 + workflow with the Z446-750-LID engaged.
5. Replacement inserts if a current set is damaged or worn.

Hermle C0175 / C0225 Bottle Pairing

The Z446-750-A225 insert is dimensioned specifically for Hermle's C0175 (175 mL) and C0225 (225 mL) conical centrifuge bottles, bottles available for order under those part numbers. The 61 mm OD envelope accommodates both bottle volumes; the 175 mL bottle is shorter than 225 mL (118 mm vs 137 mm length).

Bottle compatibility note: Third-party 175 / 225 mL conical bottles may fit the insert envelope if dimensions match (61 mm OD, 118–137 mm length range). Verify dimensions before ordering non-Hermle bottles.

Swing-Out vs Angle 175-225 mL Workflow Choice

The Z446 / Z446-K chassis offers two rotor systems for 175-225 mL conical bottle workflows:

Per-cycle throughput is the same (4 bottles); the choice depends on whether the workflow needs swing-out flat-sediment geometry (this swing-out path) or higher RCF (the angle-rotor path).

Application Profile

Cold-chain swing-out bulk-volume bottle prep at 175-225 mL: vaccine-development and bioprocess workflows that batch 4 × 175 or 225 mL conical bottles per cycle with cold-chain integrity and flat-sediment geometry.

Mid-volume cell harvest in 175-225 mL conical: pelleting cells from suspension culture batches where the swing-out flat-sediment geometry supports clean pellet recovery.

Recombinant-protein bulk prep workflows in 175-225 mL: recombinant-protein expression and harvest in bulk-volume bottle format with flat-sediment geometry.

Bioprocess intermediate prep workflows in 175-225 mL: vaccine and bioprocess intermediate concentration steps where the swing-out geometry is the protocol-specified format.

Cold-chain protein prep in bulk volume. TCA, ammonium-sulfate, organic-precipitation in 175-225 mL working volume with clean phase boundaries.

Cross-format Z446-K core facilities: labs running 175-225 mL bottle alongside other Z446-750 insert formats benefit from the cross-format insert family.

Ordering & Configuration

4-bucket symmetric loading. All 4 buckets must use matching A225 inserts for symmetric balance.

Bottle cap confirmation. All bottles must be capped securely before spinning at 4,500 rpm.

Bottle volume balance. When the 4 bottles carry different sample volumes, pre-balance pairs by total mass. Mixed 175 / 225 mL runs require pre-balance since the bottles have different filled masses.

Optional hermetic-seal engagement. When using the Z446-750-LID for biosafety workflows, confirm the lid is fully threaded onto each bucket before each spin.

Z446-750-A225 ships as 2 inserts. Bottles (Hermle C0175 175 mL or C0225 225 mL) are sold separately.

Confirm at quote: bucket pairing (Z446-750-BUK in inventory; Z446-750-LID if biosafety required), chassis pairing (Z446 or Z446-K) and power configuration, bottle format (175 mL or 225 mL conical, Hermle C0175 / C0225; verify dimensions if using third-party bottles), pack count (2 packs for full 4-bucket configuration), and lead time.

Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts.

Z446 175-225 mL bottle workflow paths:

- Z446-750-A225 (this product): swing-out on Z446-750-AC (4,346 × g, flat-sediment)

- Z446-500-A225C: angle on Z446-04500 (10,367 × g, fixed-angle 24°)

Pick the swing-out path when flat-sediment geometry matters; pick the angle path when high RCF (>10,000 × g) matters more.