The Z446-750-A225 is the 175/225 mL conical bottle insert for the Hermle Z446-750-BUK round bucket on the Z446-750-AC 4-place swing-out rotor (Z446 / Z446-K chassis) — sold as a 2-pack. Each insert holds 1 × 175 mL or 225 mL conical bottle; the 4-bucket rotor configuration takes 2 packs (4 inserts) for full symmetric loading, delivering 4 × 175/225 mL bottles per cycle (700 mL or 900 mL total) in swing-out geometry. The engineered bottles are Hermle's C0175 (175 mL) and C0225 (225 mL) conical centrifuge bottles, sold separately.
Distinct from the Z446-500-A225C insert (same bottle envelope on the Z446-04500 angle rotor at 10,367 × g) — pick the Z446-750-A225 swing-out path when flat-sediment geometry matters; pick the angle path when the higher RCF range is required.
Key Specifications
| SKU | Z446-750-A225 |
| Type | Bottle insert — 1 × 175/225 mL conical |
| Pack quantity | 2 inserts per SKU (full 4-bucket configuration takes 2 units = 4 inserts) |
| Per-insert capacity | 1 × 175 or 225 mL conical bottle |
| Per-cycle throughput (full configuration) | 4 × 175 mL = 700 mL total OR 4 × 225 mL = 900 mL total |
| Bottle format | 175 mL conical (61 mm OD, 118 mm length) OR 225 mL conical (61 mm OD, 137 mm length) |
| Engineered bottle pairing | Hermle C0175 (175 mL) or C0225 (225 mL) — sold separately |
| Compatible bucket | Z446-750-BUK (round bucket; with Z446-750-LID PC-Screw cap for sealable mode) |
| Compatible rotor | Z446-750-AC (4-place swing-out rotor body) |
| Compatible chassis | Z446, Z446-K (refrigerated), and -E voltage variants |
| Max. operating speed (with insert + bottle) | Per Z446-750-AC spec — 4,000/4,500 rpm at 3,434–4,346 × g maximum |
| Max. radius | 19.2 cm |
| Temperature at max speed (refrigerated chassis) | 4 °C |
| Geometry | Swing-out (0–90°) — flat sediment surface |
| List price | $572 (2-pack) |
Compatibility & When to Order This SKU
Insert pairing — Z446-750-BUK round bucket only. Order this SKU when:
- Adding 175 or 225 mL conical bottle workflow on the swing-out platform — order 2 units for full 4-bucket symmetric loading (700 / 900 mL total per cycle).
- Cold-chain swing-out 175-225 mL bottle prep — when the workflow benefits from swing-out flat-sediment geometry (vs the angled pellet of the Z446-04500 angle rotor) for downstream supernatant aspiration in 175/225 mL bottles.
- Mixed Z446-750 swing-out workflow consolidation — when the lab uses the same chassis for clinical-blood prep at smaller volumes AND occasional 175-225 mL bottle workflows.
- Optional BSL-2 + workflow with the Z446-750-LID engaged.
- Replacement inserts if a current set is damaged or worn.
Hermle C0175 / C0225 Bottle Pairing
The Z446-750-A225 insert is dimensioned specifically for Hermle's C0175 (175 mL) and C0225 (225 mL) conical centrifuge bottles — bottles available for order under those part numbers. The 61 mm OD envelope accommodates both bottle volumes; the 175 mL bottle is shorter than 225 mL (118 mm vs 137 mm length).
Bottle compatibility note: Third-party 175 / 225 mL conical bottles may fit the insert envelope if dimensions match (61 mm OD, 118–137 mm length range). Verify dimensions before ordering non-Hermle bottles.
Swing-Out vs Angle 175-225 mL Workflow Choice
The Z446 / Z446-K chassis offers two rotor systems for 175-225 mL conical bottle workflows:
| Rotor system | Max RCF | Geometry | Per cycle |
| Z446-750-AC + Z446-750-A225 (this insert) | 4,346 × g | Swing-out flat-sediment | 4 × 175/225 mL |
| Z446-04500 + Z446-500-A225C | 10,367 × g | Fixed-angle 24° | 4 × 175/225 mL |
Per-cycle throughput is the same (4 bottles); the choice depends on whether the workflow needs swing-out flat-sediment geometry (this swing-out path) or higher RCF (the angle-rotor path).
Application Profile
Cold-chain swing-out bulk-volume bottle prep at 175-225 mL — vaccine-development and bioprocess workflows that batch 4 × 175 or 225 mL conical bottles per cycle with cold-chain integrity and flat-sediment geometry.
Mid-volume cell harvest in 175-225 mL conical — pelleting cells from suspension culture batches where the swing-out flat-sediment geometry supports clean pellet recovery.
Recombinant-protein bulk prep workflows in 175-225 mL — recombinant-protein expression and harvest in bulk-volume bottle format with flat-sediment geometry.
Bioprocess intermediate prep workflows in 175-225 mL — vaccine and bioprocess intermediate concentration steps where the swing-out geometry is the protocol-specified format.
Cold-chain protein prep in bulk volume — TCA, ammonium-sulfate, organic-precipitation in 175-225 mL working volume with clean phase boundaries.
Cross-format Z446-K core facilities — labs running 175-225 mL bottle alongside other Z446-750 insert formats benefit from the cross-format insert family.
Ordering & Configuration
4-bucket symmetric loading. All 4 buckets must use matching A225 inserts for symmetric balance.
Bottle cap confirmation. All bottles must be capped securely before spinning at 4,500 rpm.
Bottle volume balance. When the 4 bottles carry different sample volumes, pre-balance pairs by total mass. Mixed 175 / 225 mL runs require pre-balance since the bottles have different filled masses.
Optional hermetic-seal engagement. When using the Z446-750-LID for biosafety workflows, confirm the lid is fully threaded onto each bucket before each spin.
Z446-750-A225 ships as 2 inserts. Bottles (Hermle C0175 175 mL or C0225 225 mL) are sold separately.
Confirm at quote: bucket pairing (Z446-750-BUK in inventory; Z446-750-LID if biosafety required), chassis pairing (Z446 or Z446-K) and power configuration, bottle format (175 mL or 225 mL conical, Hermle C0175 / C0225 — verify dimensions if using third-party bottles), pack count (2 packs for full 4-bucket configuration), and lead time.
Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts.
Z446 175-225 mL bottle workflow paths:
- Z446-750-A225 (this product) — swing-out on Z446-750-AC (4,346 × g, flat-sediment)
- Z446-500-A225C — angle on Z446-04500 (10,367 × g, fixed-angle 24°)
Pick the swing-out path when flat-sediment geometry matters; pick the angle path when high RCF (>10,000 × g) matters more.
