175/225 mL Conical Bottle Insert for Z446-04500 (2/pk)

The Z446-500-A225C is the 175/225 mL conical bottle insert for the Hermle Z446-04500 4 × 500 mL angle rotor on the Z446 / Z446-K chassis — sold as a 2-pack. Each insert holds 1 × 175 mL or 225 mL conical bottle; the 4-position rotor takes 2 packs (4 inserts) for full symmetric loading, delivering 4 × 175/225 mL bottles per cycle (700 mL or 900 mL total) at the host rotor's high-RCF range (10,367 × g). The C suffix denotes conical bottle envelope; the engineered bottles are Hermle's C0175 (175 mL) and C0225 (225 mL) conical centrifuge bottles, sold separately.

Key Specifications

Compatibility & When to Order This SKU

Insert pairing — Z446-04500 500 mL angle rotor only. Order this SKU when:

1. Adding 175 or 225 mL conical bottle workflow to a Z446-04500 install — order 2 units for full 4-position symmetric loading (4 bottles/cycle, 700 / 900 mL total).
2. Cold-chain bulk-volume bottle prep at high RCF — vaccine intermediate prep, recombinant-protein bottle workflows, large-volume cell harvest in 175 / 225 mL working volume.
3. Mixed 500 / 175-225 mL workflow consolidation — when the chassis primarily runs 500 mL workflows but occasional 175-225 mL processing arises.
4. Replacement inserts if a current set is damaged or worn.

Hermle C0175 / C0225 Bottle Pairing

The Z446-500-A225C insert is dimensioned specifically for Hermle's C0175 (175 mL) and C0225 (225 mL) conical centrifuge bottles — bottles available for order under those part numbers. The 61 mm OD envelope accommodates both bottle volumes (the bottles share the same 61 mm OD; 225 mL is taller than 175 mL at 137 vs 118 mm length).

Bottle compatibility note: Third-party 175 / 225 mL conical bottles may fit the insert envelope if dimensions match (61 mm OD, 118–137 mm length range). Verify dimensions before ordering non-Hermle bottles. The engineered Hermle bottle fit is the path with confirmed compatibility.

Application Profile

Bulk-volume cell harvest in 175 / 225 mL conical — pelleting cells from bulk suspension culture batches at 4 × 175/225 mL parallel throughput per cycle on the cold-chain rotor.

Vaccine-development bioprocess intermediate prep at 175 / 225 mL — vaccine-development workflows that batch 4 × 175 or 225 mL bottles per cycle for intermediate concentration steps with cold-chain integrity.

Recombinant-protein bulk prep workflows — recombinant-protein expression and harvest in 175 / 225 mL bottle format on the high-RCF refrigerated chassis.

High-RCF cold-chain bulk-volume protein workflows — TCA, ammonium-sulfate, organic-precipitation in 175 / 225 mL working volume at >10,000 × g.

Environmental and analytical sample prep at scale — water, soil-extract, sediment-supernatant prep at 175 / 225 mL bottle scale for downstream analytical workflows.

Cross-format Z446-K core facilities — labs running 175/225 mL bulk-prep alongside other Z446-04500 insert formats benefit from the cross-format adapter family.

Ordering & Configuration

4-position symmetric loading. All 4 positions on the host rotor must use matching A225C inserts for symmetric balance at 8,000 rpm.

Bottle cap confirmation. All bottles must be securely capped before spinning at 8,000 rpm — at the host rotor's high RCF, cap integrity is critical.

Bottle volume balance. When the 4 bottles carry different sample volumes, pre-balance pairs by total mass before spinning. At 175 / 225 mL bottle scale, 1–2 mL volume differences don't materially affect balance.

Mixed 175 / 225 mL runs. The same insert envelope handles both bottle volumes — but mixed-volume runs across the 4 positions require pre-balance by total mass since 175 mL bottles are lighter than 225 mL bottles when both are filled.

Z446-500-A225C ships as 2 inserts. Bottles (Hermle C0175 175 mL or C0225 225 mL) are sold separately.

Confirm at quote: host rotor (Z446-04500 in inventory), chassis pairing (Z446 or Z446-K), bottle format (175 mL or 225 mL conical, Hermle C0175 / C0225 — verify dimensions if using third-party bottles), pack count (2 packs for full 4-position configuration), and lead time.

Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts.

Z446-500 insert family (Z446-04500 4 × 500 mL angle rotor):

- Z446-500-A225C (this product) — 175/225 mL conical bottle (Hermle C0175 / C0225)

- Z446-500-A250 — 250 mL conical bottle (Hermle C1250)

- Z446-500-A250F — 250 mL flat bottle

- Z446-500-A50 — 50 mL conical

- Z446-500-A50R — 3 × 50 mL round

- Z446-500-A15 — 6 × 15 mL conical

Match the insert to the workflow's bottle format. The Z446-04500 rotor accepts 500 mL bottles directly without insert (Hermle C1500 bottle).