The Z366-06250-A225C is the 175/225 mL conical bottle insert for the Hermle Z366-06250 6 × 250 mL angle rotor on the Z366 / Z366-K chassis — sold as a 2-pack. Each insert holds 1 × 175 or 225 mL conical bottle; the 6-position rotor takes 3 packs (6 inserts) for full symmetric loading, delivering 6 × 175/225 mL bottles per cycle (1.05–1.35 L total) at the host rotor's high-RCF range. The C suffix denotes conical bottle envelope; the engineered bottles are Hermle's C0175 (175 mL) and C0225 (225 mL) conical centrifuge bottles, sold separately.
Key Specifications
| SKU | Z366-06250-A225C |
| Type | Bottle insert — 1 × 175/225 mL conical |
| Pack quantity | 2 inserts per SKU (full 6-position rotor takes 3 units = 6 inserts) |
| Per-insert capacity | 1 × 175 or 225 mL conical bottle |
| Per-cycle throughput (full configuration) | 6 × 175 mL = 1,050 mL total OR 6 × 225 mL = 1,350 mL total |
| Bottle format | 175 / 225 mL conical (61 mm OD, 118–137 mm length) |
| Engineered bottle pairing | Hermle C0175 (175 mL) or C0225 (225 mL) — sold separately |
| Compatible host rotor | Z366-06250 (6 × 250 mL angle rotor) |
| Compatible chassis | Z366, Z366-K (refrigerated), and -E voltage variants |
| Max. operating speed (with insert + bottle) | Per Z366-06250 spec — 8,000 rpm at 10,016 × g maximum |
| Max. radius | 14 cm |
| Temperature at max speed (refrigerated chassis) | 3 °C |
| Angle | 28° (fixed-angle host rotor) |
| List price | $275 (2-pack) |
Compatibility & When to Order This SKU
Insert pairing — Z366-06250 250 mL angle rotor only. This insert is engineered for the Z366-06250 250 mL position envelope. Order this SKU when:
- Adding 175 or 225 mL conical bottle workflow to a Z366-06250 install — order 3 units for full 6-position symmetric loading (6 bottles/cycle, 1.05–1.35 L total).
- Cold-chain bulk-volume bottle prep — vaccine intermediate prep, recombinant-protein bottle workflows, and large-volume cell harvest in 175 / 225 mL working volume.
- High-RCF bottle workflows — 175 / 225 mL bottle prep at >10,000 × g for tight pellet recovery in bulk volume.
- Replacement inserts if a current set is damaged or worn.
Hermle C0175 / C0225 Bottle Pairing
The Z366-06250-A225C insert is dimensioned specifically for Hermle's C0175 (175 mL) and C0225 (225 mL) conical centrifuge bottles — bottles available for order under those part numbers. The 61 mm OD × 118–137 mm length envelope accommodates both bottle volumes within the same insert (the bottles share the same 61 mm OD; 225 mL is taller than 175 mL).
Bottle compatibility note: Third-party 175 / 225 mL conical bottles may fit the insert envelope if dimensions match (61 mm OD, 118–137 mm length). Verify dimensions before ordering non-Hermle bottles. The engineered Hermle bottle fit is the path with confirmed compatibility.
Application Profile
Bulk-volume cell harvest in 175 / 225 mL conical — pelleting cells from bulk suspension culture batches at high RCF on the cold-chain rotor.
Vaccine-development bioprocess intermediate prep at 175 / 225 mL — vaccine-development workflows that batch 6 × 175 or 225 mL bottles per cycle for intermediate concentration steps with cold-chain integrity.
Recombinant-protein bulk prep workflows — recombinant-protein expression and harvest in 175 / 225 mL bottle format on the high-RCF refrigerated chassis.
High-RCF cold-chain bulk-volume protein workflows — TCA, ammonium-sulfate, organic-precipitation in 175 / 225 mL working volume at >10,000 × g.
Environmental and analytical sample prep at scale — water, soil-extract, sediment-supernatant prep at 175 / 225 mL bottle scale for downstream analytical workflows.
Cross-format Z366-K core facilities — labs running 175/225 mL bulk-prep alongside smaller-volume bottle workflows benefit from the cross-format Z366-06250 insert family.
Ordering & Configuration
6-position symmetric loading. All 6 positions must use matching A225C inserts for symmetric balance at 8,000 rpm.
Bottle cap confirmation. All bottles must be capped securely before spinning at 8,000 rpm — at 175 / 225 mL working volume, sample loss from a cap failure represents substantial sample volume.
Bottle volume balance. When the 6 bottles carry different sample volumes, pre-balance pairs by total mass before spinning. At 175 / 225 mL bottle scale, 1–2 mL volume differences don't materially affect balance, but >5 mL differences do.
Mixed 175 / 225 mL runs. The same insert envelope handles both bottle volumes — but mixed-volume runs across the 6 positions require pre-balance by total mass since 175 mL bottles are lighter than 225 mL bottles when both are filled.
Z366-06250-A225C ships as 2 inserts. Bottles (Hermle C0175 175 mL or C0225 225 mL) are sold separately.
Confirm at quote: host rotor (Z366-06250 in inventory), chassis pairing (Z366, Z366-K), bottle format (175 mL or 225 mL conical, Hermle C0175 / C0225 — verify dimensions if using third-party bottles), pack count (3 packs for full 6-position configuration), and lead time.
Warranty: 5-Year Hermle warranty (3 years full + 2 years drivetrain) on the inserts.
Z366-06250 adapter family (6 × 250 mL angle rotor):
- Z366-06250-A225C (this product) — 175/225 mL conical bottle (Hermle C0175 / C0225)
- Z366-06250-A50 / A50R — 50 mL conical / round
- Z366-06250-A30 — 2 × 30 mL
- Z366-06250-A15 — 4 × 15 mL conical
The A225C is the right pick for 175/225 mL bottle workflows — the largest non-direct bottle insert in the Z366-06250 adapter family. For 250 mL direct workflow, the rotor accepts 250 mL bottles directly without insert.
