The B3220-1000 is the 1000 mL capped Erlenmeyer flask in the hybex B3220 family — 6 flasks per pack at $243 ($40.50 per flask). The B3220 family combines Erlenmeyer flask geometry (conical body, narrow neck for shake-flask workflows) with integrated GL45 cap thread (compatible with the standard hybex bottle cap family — B3000-CAP, B3000-CAP-HTC). The capped Erlenmeyer is the right pick for shake-flask cultures requiring sealed-container storage between uses, sterile-storage protocols, and cell-culture workflows where the same flask serves both as the culture vessel and as the storage container.
Key Specifications
| SKU | B3220-1000 |
| Type | hybex Erlenmeyer flask with integrated GL45 cap |
| Volume | 1000 mL (1 L) |
| Pack quantity | 6 flasks per pack |
| Per-flask cost | $40.50 ($21.71 premium over uncapped B3110-1000 at $18.79) |
| Cap thread | GL45 (compatible with B3000-CAP family of replacement caps) |
| Sister B3220 SKU | B3220-2000 (2000 mL with GL45 cap, 1/each, $89) |
| Sister uncapped B3110 SKU | B3110-1000 (1000 mL open-mouth Erlenmeyer, 6/pk, $113) |
| Compatible replacement caps | B3000-CAP (standard PP blue), B3000-CAP-HTC (high-temp PBT red), B3000-RIN (sealing ring) |
| List price | $243 |
Why a Capped Erlenmeyer — Sealed-Container Shake-Flask Workflows
The capped Erlenmeyer combines two functions traditionally served by separate items:
| Workflow | Open-mouth Erlenmeyer (B3110) | Capped Erlenmeyer (B3220, this family) |
| Shake-flask culture | Yes | Yes |
| Sealed storage between uses | Requires separate stopper / parafilm / aluminum foil | Integrated GL45 cap |
| Sterile transfer to / from incubator | Cap-with-foil workflow | Integrated cap maintains sterility |
| Long-term sterile aliquot storage | Requires separate sealed container | Same flask serves both roles |
| Autoclave-and-store workflow | Workable but cap-management overhead | Streamlined — autoclave with vented cap, switch to solid cap for storage |
| Cap-accessory ecosystem | None integrated | Compatible with B3000-CAP family (high-temp / standard / dispensing accessories) |
Pick the capped Erlenmeyer (B3220-1000) when:
- Workflow involves shake-flask cultures requiring sealed-container storage between uses
- Sterile transfer workflows where integrated cap maintains sterility better than parafilm / foil
- Long-term sterile aliquot storage in the same vessel as culture
- Cell-culture workflows where the flask serves both as culture vessel AND as storage container
- Pairs with B3000-CAP cap-accessory ecosystem — high-temp caps for sterilization, dispensing caps for downstream aliquoting
Pick the uncapped B3110-1000 when standard open-mouth shake-flask workflow is the routine — $130 saving over the capped version when the integrated cap isn't required.
B3220 Capped Erlenmeyer Family — Pack-Size
| SKU | Volume | Pack | Per-flask cost | List price |
| B3220-1000 (this product) | 1000 mL | 6/pk | $40.50 | $243 |
| B3220-2000 | 2000 mL | 1/each | $88.67 | $89 |
The B3220 family covers only the larger volumes (1000 mL / 2000 mL) — the capped-flask format isn't offered in the smaller volumes (50 / 125 / 250 mL) where standard B3110 open-mouth + separate stoppers is the typical workflow.
Capped vs Uncapped — Pricing Premium
| Volume | B3110 (uncapped) | B3220 (capped, this family) | Premium |
| 1000 mL | $18.79/flask (B3110-1000, 6/pk $113) | $40.50/flask (B3220-1000, 6/pk $243) | +$21.71/flask (~115 % premium for integrated cap) |
| 2000 mL | $34.44/flask (B3110-2000, 1/each $34) | $88.67/flask (B3220-2000, 1/each $89) | +$54.23/flask (~158 % premium) |
The capped premium is meaningful — only justified when the integrated GL45 cap delivers workflow value over the open-mouth alternative.
Application Profile
Shake-flask culture + sterile storage workflows — cell culture, microbial culture, protein-expression workflows requiring sealed-container storage between use cycles.
Sterile transfer protocols — workflows transferring flasks between incubators, biosafety cabinets, and bench storage where integrated cap maintains sterility.
Long-term sterile aliquot storage — aliquots stored in the same flask as the culture, eliminating transfer-step contamination risk.
Pharmaceutical / biotech workflows — high-stakes sterility workflows where cap-and-store integration matters.
Cell-culture facility procurement — TC-room workflows where cap-managed sterility supports cell-culture protocol consistency.
Method-development workflows — protocol-development requiring same-vessel culture-and-storage workflow.
Multi-investigator shared facility — capped flasks reduce sterility-handling variation across investigators.
Ordering & Configuration
Pair with cap accessories per workflow. Standard B3000-CAP (blue PP) for routine; B3000-CAP-HTC (red PBT) for high-temperature sterilization; B3000-RIN for sealing-ring replacement.
Confirm autoclave protocol. Like the bottle family, capped Erlenmeyers should be autoclaved with vented caps (loosened solid cap or vented-cap variant) to prevent pressure buildup.
Confirm cell-culture workflow context. The capped Erlenmeyer's sterile-storage benefit is most valuable in TC-room workflows; standard chemistry workflows may not justify the premium.
Confirm at quote: flask material, cap material (PP standard vs PBT high-temp), autoclave protocol (vented-cap pairing), intended workflow context, and lead time.
Warranty: Per Benchmark Scientific standard consumable terms.
hybex Erlenmeyer family — pick capped vs open-mouth:
- B3220 (capped, this family) — Erlenmeyer + integrated GL45 cap — sealed-container workflows
- B3220-1000 (this product) — 1000 mL × 6/pk ($243, $40.50/flask)
- B3220-2000 — 2000 mL × 1/each ($89, $88.67/flask)
- B3110 (open-mouth) — standard Erlenmeyer — open-mouth shake-flask workflows at lower per-flask cost
Pick B3220 when integrated GL45 cap delivers workflow value (sealed storage, sterile transfer); B3110 for open-mouth workflows at lower cost.
