The B4000-28-120 is the largest autoclave in the BioClave family — 28 L chamber capacity at $13,895 ($6,350 step over the BioClave 18 at $7,545). Same validated 121 °C steam-sterilization mechanism as the smaller sisters, scaled to production-scale workflows — large-batch media sterilization, large-batch glassware sterilization, multi-investigator shared-facility throughput, production-scale biohazard waste decontamination at facility scale.
Universe naming-convention note: The BioClave 28 uses -120 / -220 SKU suffixes (denoting plug type — 120 V US vs 220 V international) rather than the "" / -E suffix pattern used by the Mini and 18 sisters. Both -120 and -220 SKUs ship at the same $13,895 price.
For full chemistry rationale (validated sterility, BioClave vs other decontamination modalities, electrical / water / facility planning, safety considerations), see the B4000-M (BioClave Mini) anchor page and the B4000-18 mid-size parent page.
Voltage options: This unit can be ordered with European voltage requirements (230V/50Hz). Contact us to order.
Key Specifications
| SKU | B4000-28-120 |
| Type | Research-grade benchtop autoclave, production-scale (largest in BioClave family) |
| Capacity | 28 L chamber (largest in family) |
| Sterilization mechanism | Pressurized steam at 121 °C |
| Voltage | 115 V or 230 V (universal-input chassis); -120 SKU suffix denotes US plug variant |
| Sister voltage variant | B4000-28-220 (220 V international plug, $13,895) |
| Sister capacity SKUs | B4000-M (Mini, $5,453), B4000-18 (18 L, $7,545) |
| Compatible accessories | B4000-P (printer, $387), B4000-PA (printer paper, $52), B4000-PM-KIT (preventative maintenance, $100) |
| List price | $13,895 |
| Safety flag | High-pressure steam hazard + thermal hazard (121 °C) |
Why the 28 L Capacity — Production-Scale Workflows
The 28 L chamber is the largest BioClave — it supports workflows that exceed the 18 L chamber's capacity: production-scale media sterilization (8–15+ standard 1 L media bottles per cycle), large-batch glassware sterilization (15–25+ flasks or bottles per cycle), multi-investigator shared-facility throughput consolidating multiple workflows into single cycles, production-scale biohazard waste decontamination at facility-scale BSL2 volume, cell-culture facility bulk media prep, and high-stakes pharmaceutical or biotech sterility workflows at scale.
Pick the BioClave 28 (B4000-28-120) when:
- Production-scale sterilization is the workflow throughput driver
- Multi-investigator shared facility consolidating multiple workflows into single autoclave cycles
- Cell-culture facility / production-prep procurement at facility scale
- The 18 L chamber has been throughput-limiting (frequent batch-by-batch sequencing)
Stay with B4000-18 (18 L, $7,545) when mid-throughput workflows match the smaller chamber.
Stay with B4000-M (Mini, $5,453, 200–240 V only) when small-batch workflows + dedicated 200–240 V circuit match the procurement.
BioClave Family — Capacity Comparison
| SKU | Capacity | Voltage | List price |
| B4000-M / -M-E | Mini (smallest) | 200–240 V only | $5,453 |
| B4000-18 / -18-E | 18 L | 115 V or 230 V | $7,545 |
| B4000-28-120 / -220 (this product / sister) | 28 L (largest) | 115 V or 230 V | $13,895 |
Application Profile
Production-scale media sterilization — bulk media prep at facility scale.
Large-batch glassware sterilization — high-throughput glassware sterilization workflows.
Multi-investigator shared-facility procurement — consolidating multiple investigators' workflow into shared cycles.
Production-scale biohazard waste decontamination — facility-scale BSL2 waste cycles.
Cell-culture facility procurement — bulk-media-prep at production scale for cell-culture work.
Pharmaceutical / biotech bench standardization — high-stakes sterility workflows at facility scale.
Multi-cycler facility procurement — facilities consolidating sterilization onto a single large autoclave vs operating multiple smaller ones.
Educational laboratory teaching at scale — graduate / undergraduate program-wide sterilization at production-scale procurement.
Frequently Asked Questions
What is the chamber capacity of the BioClave 28? 28 L — the largest chamber in the BioClave autoclave family, sized for production-scale workflows.
How does it compare to the smaller BioClaves? It lists at $13,895 — a $6,350 step over the 18 L BioClave 18 ($7,545), and larger than the BioClave Mini ($5,453). All share the validated 121 °C steam-sterilization mechanism.
What does the -120 suffix mean? It denotes plug type — the -120 is the US-plug variant and the B4000-28-220 is the 220 V international-plug variant. Both are universal-input chassis and ship at the same $13,895.
What is the sterilization mechanism? Pressurized steam at 121 °C. Note the high-pressure steam and thermal (121 °C) hazards, which are more pronounced at this largest chamber volume.
What accessories are available? The B4000-P printer ($387), B4000-PA printer paper ($52), and the B4000-PM-KIT preventative-maintenance kit ($100).
Ordering & Configuration
Confirm electrical infrastructure. 115 V or 230 V — confirm circuit amperage matches the larger 28 L autoclave's draw at order.
Confirm bench-space and floor-space. The 28 L chassis has substantially larger footprint than the smaller BioClave sisters — confirm available facility space.
Confirm water source and drainage at production-scale flow rates. The 28 L chamber consumes more water per cycle than smaller sisters — confirm water-supply capacity at order.
Confirm regulatory compliance plan, accessory pairing, preventative maintenance schedule — see B4000-M parent page for full procurement-planning detail.
Confirm at quote: voltage and plug type (-120 vs -220), facility-space, water supply / drainage at production-scale flow, regulatory compliance, chamber-load sizing, preventative maintenance, training, warranty, and lead time.
Warranty: Per Benchmark Scientific standard capital-equipment terms.
Safety note: the BioClave 28 operates with pressurized steam at 121 °C+ at the largest BioClave chamber volume — meaning more thermal mass and longer pressure-equalization cycle than smaller sisters. Confirm operator safety training, PPE, door-interlock verification, and post-cycle pressure-equalization protocol especially carefully at the production-scale chamber.
BioClave family — pick the right size:
- B4000-M / -M-E — Mini ($5,453, 200–240 V only)
- B4000-18 / -18-E — 18 L ($7,545, 115/230 V) — mid-throughput
- B4000-28-120 (this product) / -220 — 28 L ($13,895, 115/230 V) — production-scale / facility-wide
Pick the BioClave 28 for production-scale sterilization at facility scale; sister sizes cover smaller-batch workflows. See B4000-M anchor for full BioClave family chemistry / safety detail.